Transboundary and Emerging Diseases,
Год журнала:
2024,
Номер
2024(1)
Опубликована: Янв. 1, 2024
Feline
coronavirus
(FCoV)
is
an
enveloped,
positive‐sense
RNA
virus,
which
widespread
among
feline
populations,
and
can
cause
a
fatal
serious
disease
called
infectious
peritonitis
(FIP).
According
to
the
differences
of
antigen
genetic
composition,
FCoV
consists
two
genotypes,
I
II.
In
this
study,
we
have
isolated
identified
strain
named
HL2019.
Based
on
complete
genome
HL2019,
phylogenetic
analysis
showed
that
HL2019
formed
in
cluster
more
closed
human
229E
(HCoV
229E)
HCoV
NL63,
while
stains
distantly
related
II
strains.
Analyzing
with
RDP4
Simplot
software
virus
recombinant
by
China/ZJU1709
Netherlands/UU16
Furthermore,
pathogenicity
was
evaluated
9–12‐month‐old
cats.
Two
three
challenged
cats
developed
clinical
signs
died
at
28‐day
postchallenge
(dpc).
Real‐time
polymerase
chain
reaction
(PCR)
has
broad
tissue
tropism,
especially
duodenum
viral
load
up
10
4
copies/mg.
summary,
our
data
show
successfully
highly
pathogenic
Veterinary Research,
Год журнала:
2022,
Номер
53(1)
Опубликована: Май 31, 2022
Abstract
Porcine
circovirus
type
4
(PCV4)
is
a
newly
emerging
virus,
with
both
PCV4
genomic
DNA
and
specific
antibodies
detected
in
swine
herds
several
provinces
China
South
Korea.
Although
the
virus
was
first
identified
2019
Hunan,
China,
retrospective
research
suggests
that
serum
samples
collected
as
early
2008
were
positive
for
antibody.
Infections
only
or
co-infections
other
pathogens
have
been
associated
clinical
manifestations,
but
its
pathogenesis
remains
to
be
determined.
The
purpose
of
this
review
following:
(1)
characterize
epidemiology
by
assessing
evolutionary
dynamics
genetic
diversity
strains
circulating
herds;
(2)
reconstruct
computerized
3D
model
analyze
Cap
properties;
(3)
summarize
current
evidence
PCV4-associated
clinical-pathological
manifestations.
origin
apparently
distinct
from
PCV,
based
on
analysis
phylogenetic
trees.
Of
note,
shares
an
ancient
common
ancestor
mink
circoviruses.
Furthermore,
amino
acid
residue
at
position
27
key
benchmark
distinguish
PCV4a
(
S)
PCV4b
N),
currently
available,
variation
may
alter
antigenicity.
In
addition,
capsid
surface
has
characteristics
increased
polar
residues,
compared
PCV2,
which
raises
possibility
target
negatively
charged
host
receptors
promote
infection.
Further
studies
are
required,
including
isolation
culture,
more
detailed
characterization
molecular
herds.
Emerging infectious diseases,
Год журнала:
2023,
Номер
29(5)
Опубликована: Апрель 18, 2023
We
identified
a
novel
circovirus
(human-associated
2
[HuCV2])
from
the
blood
of
intravenous
drug
users
in
China
who
were
infected
with
HIV-1,
hepatitis
C
virus,
or
both.
HuCV2
is
most
closely
related
to
porcine
3.
Our
findings
underscore
risk
for
and
other
emerging
viruses
among
this
population.
Emerging infectious diseases,
Год журнала:
2024,
Номер
30(10)
Опубликована: Сен. 25, 2024
We
identified
a
novel
human
circovirus
in
an
immunocompromised
66-year-old
woman
with
sudden
onset
of
self-limiting
hepatitis.
detected
1
(HCirV-1)
transcripts
hepatocytes
and
the
HCirV-1
genome
long-term
patient's
blood,
stool,
urine.
is
emerging
pathogen
that
persists
susceptible
patients.
Veterinary Sciences,
Год журнала:
2022,
Номер
10(1), С. 29 - 29
Опубликована: Дек. 31, 2022
Porcine
circovirus
disease
(PCVD)
caused
by
porcine
(PCV)
is
an
important
swine
that
characterized
dermatitis,
nephropathy
syndrome,
and
reproductive
disorders
in
sows.
However,
PCV2,
PCV3,
or
PCV4
hard
to
distinguish,
so
a
rapid
sensitive
detection
method
urgently
needed
differentiate
these
three
types.
In
this
study,
four
pairs
of
specific
primers
the
corresponding
probes
for
PCV
2,
-3,
-4,
endogenous
gene
β-Actin
as
positive
internal
reference
index,
were
designed
establish
TaqMan
multiplex
real-time
PCR
(qPCR)
assay
simultaneous
differential
diagnosis
different
types
viruses.
The
results
showed
has
good
specificity
no
cross-reactivity
with
other
viral
pathogens.
Furthermore,
it
high
sensitivity,
limit
101
copies/μL,
reproducibility,
intra-
inter-group
coefficients
variation
below
2%.
Subsequently,
535
clinical
samples
suspected
sow
collected
from
Shandong,
Zhejiang,
Anhui,
Jiangsu
provinces
2020
2022
analyzed
using
established
assay.
individual
rates
31.03%,
30.09%,
30.84%,
respectively;
mixed
infection
PCV2
PCV4,
PCV3
rate
was
28.22%.
This
indicated
provides
convenient
tool
differentiation
pig
farms
East
China.
Our
findings
highlight
there
are
China,
which
makes
prevention
control
difficult.
Microbiology Spectrum,
Год журнала:
2023,
Номер
11(2)
Опубликована: Фев. 2, 2023
This
study
is
the
first
to
report
presence
of
PCV4
in
dogs
worldwide,
and
complete
genome
sequence
was
obtained
from
a
dog
affected
with
diarrhea.
Three
genotypes
strains
(PCV4a,
PCV4b,
PCV4c)
were
determined,
as
supported
by
specific
amino
acid
markers
(V239L
for
open
reading
frame
1
[ORF1]
N27S
R28G
M212L
ORF2).
