Genotype Detection of Circovirus Type 2 (PCV2) in Swine Clinical Samples from Brazil DOI Creative Commons

Rovian Miotto,

Caroline Pissetti, Luiz Carlos Bordin

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Авг. 28, 2023

Abstract Porcine circovirus disease (PCVD) is one of the most impactful diseases in commercial pig production. It caused by infection a small, circular DNA virus, porcine type 2 (PCV2). Currently, four types PCV have been identified: PCV1, PCV2, PCV3, and PCV4. PCV2 classified into 9 different genotypes (a-i). Although there widespread use vaccines for PCVD not new disease, its presentation has changed. In addition, also evolved genetically, requiring monitoring vaccinated herds clinical genotypic aspects. The objective this study was to identify namely, PCV2a, PCV2b, PCV2d. Additionally, aimed coinfections PCV3 coinfections. Herein, 333 samples pigs that tested positive from various tissues, states Brazil, production phases were analyzed. Of those, 266 genotyped, PCV2b frequent genotype found 56.77% samples, mainly nursery samples. However, PCV2d present 33.10% finishing breeding sows. PCR used compared kit effective genotyping. This shows it important conventional demonstrated good option this. remains prevalent farms Brazil continues cause disease. necessary state commonly are PCV2a. Even though PCV2a provide heterologous protection other genotypes, adequate diagnosis need updating currently circulating must be considered.

Язык: Английский

The virome investigation of the globally endangered Eld’s deer (Rucervus eldii) on Hainan Island, China DOI Creative Commons
Haoxiang Su, Dandan Zheng, Zihan Li

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 11, 2025

Eld's deer (Rucervus eldii) is a rare and globally endangered tropical Southeast Asian species. There no research on pathogens in Hainan, China. This study aimed to understand the virus diversity novel viruses deer, provided important epidemiological baseline information for conservation of this 33 nasal swabs, anal 9 wound (bitten by ticks) swabs were collected from wild nature reserve which constituted into 5 pools. Based next-generation sequencing (NGS) macrogenomic analysis, there differences pools viral reads, while overall reads closely related mammals. The papillomavirus (PsPV-HMU-1) Circular Rep-encoding (replication-associated protein encoding) single-stranded DNA (CRESS DNA) (PsaCV-HMU-1) identified with amino acid homology less than 77.20% L1 45.43% rep, respectively. PsPV-HMU-1 PsaCV-HMU-1 are relatively independent their phylogenetic trees, prevalence 24.24% (8/33) 3.03% (1/33) Our results expanded genomic host range, implied that it necessary continued surveillance order pathogenicity potential cross-species transmission deer.

Язык: Английский

Процитировано

0
Evidence of Porcine Circovirus Type 2 (PCV2) Genetic Shift from PCV2b to PCV2d Genotype in Sardinia, Italy DOI Creative Commons

Silvia Dei Giudici,

L. Mura,

Piero Bonelli

и другие.

Viruses, Год журнала: 2023, Номер 15(11), С. 2157 - 2157

Опубликована: Окт. 26, 2023

Porcine Circovirus type 2 (PCV2) is the etiological agent of a disease syndrome named (PCVD), representing an important threat for pig industry. The increasing international trade live animals and development intensive farming seem to have sustained spreading PCVD on global scale. Recent classification criteria allowed identification nine different PCV2 genotypes (PCV2a–i). PCV2a was first genotype detected with highest frequency from late 1990s 2000, which then superseded by PCV2b (first shift). An ongoing shift now determining prevalence rates PCV2d, in replacement PCV2b. In Italy, complete not evidenced yet. present study carried out 369 samples originating domestic pigs, free-ranging wild boars collected Sardinia between 2020 2022, aim update last survey performed during 2009–2013. Fifty-seven ORF2 sequences were obtained, phylogenetic network analyses that 56 57 strains belong PCV2d only one strain PCV2b, thus showing occurrence Sardinia.

Язык: Английский

Процитировано

5
Detection and Complete Genomic Analysis of Porcine circovirus 3 (PCV3) in Diarrheic Pigs from the Dominican Republic: First Report on PCV3 from the Caribbean Region DOI Creative Commons
Kerry Gainor,

Yussaira Castillo Fortuna,

Angeline Steny Alakkaparambil

и другие.

Pathogens, Год журнала: 2023, Номер 12(2), С. 250 - 250

Опубликована: Фев. 4, 2023

The increasing detection of Porcine circovirus 3 (PCV3, family Circoviridae) in clinically ill pigs worldwide has raised concerns on the implications virus porcine health and pork industry. Although production constitutes an important component livestock economy is a major source animal protein Caribbean Islands, there are no reports PCV3 from region so far. In present study, was detected 21% (21/100) diarrheic (sampled at three farms) nation Dominican Republic (DR). sample size varied between age groups, highest rates (35.3% each, respectively) were observed piglets growers. Co-infections with PCV2 adenovirus 38.09% 9.52% positive samples, respectively. complete genomes 11 DR strains analyzed revealing unique deletion (corresponding to nucleotide residue position 1165 reference sequences) one sequences. Based sequence identities phylogenetic analysis (open reading frame 2 genome sequences), assigned genotype PCV3a, shared high homologies (>98% identities) themselves those other PCV3a (Clade-1) strains, corroborating previous observations genetic stability worldwide. To our knowledge, this first report molecular characterization region, providing insights into expanding global distribution virus, even isolated geographical regions (the Island Hispaniola). Our findings warrant further investigations epidemiology economic diarrhea clinical conditions across region.

Язык: Английский

Процитировано

2
First Report on Detection and Complete Genomic Analysis of a Novel CRESS DNA Virus from Sea Turtles DOI Creative Commons
Kerry Gainor, Kimberly M. Stewart,

Angela Picknell

и другие.

Pathogens, Год журнала: 2023, Номер 12(4), С. 601 - 601

Опубликована: Апрель 15, 2023

To date, only a handful of viruses have been identified in sea turtles. Although eukaryotic circular Rep (replication initiation protein)-encoding single-stranded DNA (CRESS DNA) reported from wide variety terrestrial species, and some these associated with clinical conditions certain animals, limited information is available on CRESS marine life. The present study aimed to investigate the presence In study, two (samples T3 T33) 34 cloacal samples 31 turtles (found ocean waters around Caribbean Islands St. Kitts Nevis) tested positive for by pan-rep nested PCR assay. partial sequence shared 75.78% deduced amino acid (aa) identity that virus (classified under family Circoviridae) mollusk. On other hand, complete genome (2428 bp) T33 was determined an inverse genomic organization mirrored those type II viral genomes cycloviruses, characterized putative "origin replication" 5'-intergenic region, Capsid (Cap)- Rep-encoding open reading frame virion-sense- antisense-strand, respectively. (322 aa) retained conserved "HUH endonuclease" "super 3 helicase" domains pairwise aa identities ~57% unclassified benthic sediment mollusks. Phylogenetically, formed distinct branch within isolated cluster viruses. Cap (370 maximum 30.51% capybara. Except blood sample negative viruses, tissue were not Therefore, we could establish whether strains infected or dietary origin. our knowledge, this first report detection turtles, adding yet another animal species rapidly expanding host range Complete analysis novel, virus, providing insights into high genetic diversity between phylum Cressdnaviricota. Considering are at-risk extensive studies discovery, surveillance, pathogenesis animals utmost importance.

Язык: Английский

Процитировано

1
Genotype Detection of Circovirus Type 2 (PCV2) in Swine Clinical Samples from Brazil DOI Creative Commons

Rovian Miotto,

Caroline Pissetti, Luiz Carlos Bordin

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Авг. 28, 2023

Abstract Porcine circovirus disease (PCVD) is one of the most impactful diseases in commercial pig production. It caused by infection a small, circular DNA virus, porcine type 2 (PCV2). Currently, four types PCV have been identified: PCV1, PCV2, PCV3, and PCV4. PCV2 classified into 9 different genotypes (a-i). Although there widespread use vaccines for PCVD not new disease, its presentation has changed. In addition, also evolved genetically, requiring monitoring vaccinated herds clinical genotypic aspects. The objective this study was to identify namely, PCV2a, PCV2b, PCV2d. Additionally, aimed coinfections PCV3 coinfections. Herein, 333 samples pigs that tested positive from various tissues, states Brazil, production phases were analyzed. Of those, 266 genotyped, PCV2b frequent genotype found 56.77% samples, mainly nursery samples. However, PCV2d present 33.10% finishing breeding sows. PCR used compared kit effective genotyping. This shows it important conventional demonstrated good option this. remains prevalent farms Brazil continues cause disease. necessary state commonly are PCV2a. Even though PCV2a provide heterologous protection other genotypes, adequate diagnosis need updating currently circulating must be considered.

Язык: Английский

Процитировано

0