Ultrasensitive and Specific Clustered Regularly Interspaced Short Palindromic Repeats Empowered a Plasmonic Fiber Tip System for Amplification-Free Monkeypox Virus Detection and Genotyping

ACS Nano, Год журнала: 2023, Номер 17(13), С. 12903 - 12914

Опубликована: Июнь 29, 2023

The urgent necessity for highly sensitive diagnostic tools has been accentuated by the ongoing mpox (monkeypox) virus pandemic due to complexity in identifying asymptomatic and presymptomatic carriers. Traditional polymerase chain reaction-based tests, despite their effectiveness, are hampered limited specificity, expensive bulky equipment, labor-intensive operations, time-consuming procedures. In this study, we present a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-based platform with surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor. compact CRISPR-SPR-FT biosensor, 125 μm diameter, offers high stability portability, enabling exceptional specificity diagnosis precise identification of samples fatal mutation site (L108F)

Язык: Английский

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A comprehensive review of monkeypox virus and mpox characteristics

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Март 6, 2024

Monkeypox virus (MPXV) is the etiological agent of monkeypox (mpox), a zoonotic disease. MPXV endemic in forested regions West and Central Africa, but has recently spread globally, causing outbreaks multiple non-endemic countries. In this paper, we review characteristics virus, including its ecology, genomics, infection biology, evolution. We estimate by phylogenomic molecular clock that B.1 lineage responsible for 2022 mpox been circulation since 2016. interrogate host-virus interactions modulate signal transduction, pathogenesis, host immune responses. highlight changing pathophysiology epidemiology summarize recent advances prevention treatment mpox. addition, identifies knowledge gaps with respect to disease, suggests future research directions address gaps, proposes One Health approach as an effective strategy prevent current epidemics

Язык: Английский

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Monkeypox (mpox) in immunosuppressed patients

F1000Research, Год журнала: 2023, Номер 12, С. 127 - 127

Опубликована: Апрель 3, 2023

The World Health Organization (WHO) proclaimed a public health emergency in July 2022 due to the emergence of Mpox (formerly monkeypox) while globe was still dealing with COVID-19 epidemic. The characteristics mpox immunocompetent individuals are well-characterized, despite difficulties diagnostics, immunization, and access treatment that persist low-income countries. Patients weakened immune systems more likely spread an illness die from it than healthy people because they cannot mount protective response against it, such as neutralizing IgG poxvirus-specific Th1 response. A warning on severe who immunocompromised Human Immunodeficiency virus (HIV) other illnesses released by U.S. Centers for Disease Control Prevention (CDC) September 29, 2022. advice does not specifically include primary immunodeficiency, but define immunocompromising disorders “having autoimmune disease immunodeficiency clinical component”. Both those systems, immunosuppressed, older people, children, etc., have encountered serious issues, latter group is do so. According advisory, “of manifestations whom CDC has been consulted, majority had HIV CD4 counts 200 cells/ml, indicating substantial immunosuppression”. However, new cases expected be discovered, especially countries limited diagnosis, treatment, prevention, where large percentage mpox-infected population also advanced infection. Thus, further research always needed determine best way treat people. In this context, we discussed /reviewed presentation, available options current preventive guidelines patients.

Язык: Английский

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Monkeypox (mpox) in immunosuppressed patients

F1000Research, Год журнала: 2023, Номер 12, С. 127 - 127

Опубликована: Фев. 2, 2023

The World Health Assembly declared that smallpox had been completely eradicated from the human population in 1980. Monkeypox, a zoonosis native to damp forested regions West and Central Africa, is illness most comparable clinically immunologically. Both illnesses could be prevented by vaccine. Although monkeypox virus less effective disease than virus, it now spread among populations if not population-wide immunity developed. A health warning on severe people who are immunocompromised due Human Immunodeficiency (HIV) other was released U.S. Centers for Disease Control Prevention (CDC) September 29, 2022. The advise does specifically include primary immunodeficiency, but define immunocompromising disorders as “having autoimmune with immunodeficiency clinical component”. documented signs of widespread rashes secondary fungal or bacterial skin infections tissue death (necrosis), intestine obstruction, difficulties heart, lungs, urinary system, nervous system. those healthy immune systems weakened systems, such immunosuppressed, older people, children, etc., have encountered serious issues, latter group more likely do so. According advisory, “of manifestations whom CDC has consulted, majority HIV CD4 counts 200 cells/ml, indicating substantial immunosuppression”. current article goes into great detail about occurring patients preventive guidelines.

Язык: Английский

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Mpox: epidemiology, clinical manifestations and recent developments in treatment and prevention

Expert Review of Anti-infective Therapy, Год журнала: 2023, Номер 21(6), С. 641 - 653

Опубликована: Апрель 27, 2023

The 2022 Mpox outbreak is the largest viral recorded outside of Africa. recent rise in human outbreaks has led to perception that Mpox, an emerging zoonotic disease, potential for epidemic spread. Healthcare practitioners are familiarizing themselves with varied clinical manifestations and therapeutic approaches this virus while public health organizations seek limit treat those affected. In light worldwide upsurge, we have assembled a review on streamline information access healthcare professionals.Within article, you will find overview outlining virology, epidemiology, symptoms, diagnosis, management Mpox. Furthermore, provide analysis infective mechanisms strategies children adolescents current literature.The spread non-endemic regions elicited alarm due lack easily available regarding virus. While continue understand its possible evolution increased education knowledge among providers crucial. Through creation reviews compile crucial into centralized location, can assist lessening harmful effects through caution education.

Язык: Английский

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Virtual screening and computational simulation analysis of antimicrobial photodynamic therapy using propolis-benzofuran A to control of Monkeypox

Photodiagnosis and Photodynamic Therapy, Год журнала: 2022, Номер 41, С. 103208 - 103208

Опубликована: Ноя. 20, 2022

Язык: Английский

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Repurposing doxycycline for the inhibition of monkeypox virus DNA polymerase: a comprehensive computational study

In Silico Pharmacology, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 13, 2025

Язык: Английский

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Infezioni in Medicina

Infezioni in Medicina, Год журнала: 2023, Номер 31(2)

Опубликована: Июнь 1, 2023

Monkeypox (Mpox) is an emerging viral disease caused by the monkeypox virus (MPXV), a double-stranded DNA member of genus Orthopoxvirus, first reported in humans 1970. Since May 2022, global spread infection has occurred that World Health Organization (WHO) declared public health emergency. In view threat, efforts have been devoted to bolstering as well identifying viable therapeutic modalities. People living with HIV may be at increased risk adverse outcomes and require antiviral treatment. With regard antiretroviral drugs agents, anticipated drug reactions do not preclude co-administration combined therapy antivirals for mpox. More data on treatment recommendations efficacy patients immunodeficiency due needed. this review, tecovirimat, cidofovir brincidofovir - agents activity against MPXV other Orthopoxviruses are reviewed, their utilization vulnerable patient groups affected mpox such people possible gaps future research. Tecovirimat inhibitor Orthopoxvirus VP37 envelope wrapping protein thus rendering enveloped formation impossible. Cidofovir its prodrug interfere synthesis through polymerase inhibition. Ongoing research intensified verify applicability.

Язык: Английский

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Fluorescent Immunochromatographic Assay (FICA) for Monkeypox Virus

Analytical Letters, Год журнала: 2023, Номер 57(13), С. 2118 - 2131

Опубликована: Дек. 2, 2023

Monkeypox, a rare zoonotic disease classified as category B infectious disease, is caused by the monkeypox virus (MPXV), which has broad host range. Since May 2022, human MPXV infection outbreak spread to more than 100 countries, and China reported 106 infections between June 2 30, 2023. Therefore, rapid effective detection essential control prevent its transmission. In this study, rapid, cost-effective, convenient fluorescent immunochromatographic assay (FICA) for antigen A35R was developed. Under optimal reaction conditions, visual limit of proposed 7.5 ng/mL completed within 15 min. There no cross-reactivity with norovirus (NV) or dengue (DENV) controls. Additionally, clinical application method demonstrated testing serum samples spiked A35R. FICA faster traditional enzyme-linked immunosorbent assays (ELISA) polymerase chain (PCR), hence suitable early screening infection. summary, could realize low-cost, sensitive, specific hold potential prospects in settings.

Язык: Английский

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Human-to-Human Transmission of Monkeypox Virus Old Virus with a New Face

Journal of Pure and Applied Microbiology, Год журнала: 2022, Номер 16(suppl 1), С. 3048 - 3061

Опубликована: Дек. 30, 2022

The outbreak of Monkeypox was declared a public health emergency worldwide concern by WHO following the (COVID-19) pandemic. number reported cases both suspicion and confirmation has increased in recent years, from over 19,000 between 2000 2019 to 15,600 2021 2022, day monkeypox have been 12 member states three regions. As May 21, 92 confirmed 28 suspected had more than countries. By 21st June total 2677 recorded UK other European non-European According an extensive literature survey, registered MPXV 59,147 January 2022 September 14th, demonstrating that can spread significantly amongst people may as result pose serious threat with international repercussions. In clade II virus is currently occurring outside Africa 25,047 August 2nd, 2022. Here, we review current better understanding, studies on monkeypox, including its history, scenario, epidemiology, causative agent, symptoms, diagnosis, treatment, limitations, new face unusual attributed transformation transmission also discussed case this article.

Язык: Английский

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