Pharmaceuticals,
Год журнала:
2024,
Номер
17(12), С. 1680 - 1680
Опубликована: Дек. 12, 2024
Background/Objectives:
Infectious
diseases
represent
a
serious
threat
due
to
rising
antimicrobial
resistance,
particularly
among
multidrug-resistant
bacteria
and
influenza
viruses.
Metal-based
complexes,
such
as
N-heterocyclic
carbene–gold
(NHC–gold)
show
promising
therapeutic
potential
their
ability
inhibit
various
pathogens.
Methods:
Eight
NHC–gold
complexes
were
synthesized
tested
for
antibacterial
activity
against
Escherichia
coli,
Enterococcus
faecalis,
Staphylococcus
aureus,
anti-influenza
in
lung
bronchial
epithelial
cells
infected
with
virus
A/H1N1.
Antibacterial
was
assessed
through
the
determination
of
minimum
inhibitory
concentration
(MIC)
bactericidal
(MBC),
while
viral
load
quantified
using
qRT-PCR.
Results:
Complexes
3,
4,
6
showed
significant
at
concentrations
10–20
µg/mL.
Additionally,
these
significantly
reduced
load,
3
4
markedly
inhibiting
replication.
Conclusions:
These
findings
support
use
combined
antiviral
therapies,
representing
an
attractive
option
fighting
resistant
infections.
Viral
infections
continue
to
threaten
human
health.
It
remains
a
major
challenge
efficiently
inhibit
viral
infection
while
avoiding
secondary
injury.
Here,
we
designed
multifunctional
nanoplatform
(termed
as
ODCM),
prepared
by
oseltamivir
phosphate
(OP)-loaded
polydopamine
(PDA)
nanoparticles
camouflaged
the
macrophage
cell
membrane
(CM).
OP
can
be
loaded
onto
PDA
through
π-π
stacking
and
hydrogen
bonding
interactions
with
high
drug-loading
rate
of
37.6%.
In
particular,
biomimetic
accumulate
actively
in
damaged
lung
model
infection.
At
site,
consume
excess
reactive
oxygen
species
simultaneously
oxidized
degraded
achieve
controlled
release
OP.
This
system
exhibits
enhanced
delivery
efficiency,
inflammatory
storm
suppression,
replication
inhibition.
Therefore,
exerts
outstanding
therapeutic
effects
improving
pulmonary
edema
protecting
injury
mouse
influenza
A
virus
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 17, 2025
Human
Immunodeficiency
Virus
Type
1
(HIV)
set-point
viral
load
is
a
strong
predictor
of
disease
progression
and
transmission
risk.
A
recent
genome-wide
association
study
in
individuals
African
ancestries
identified
region
on
chromosome
significantly
associated
with
decreased
HIV
load.
Knockout
the
closest
gene,
CHD1L,
enhanced
replication
vitro
myeloid
cells.
However,
it
remains
unclear
if
spVL
variants
are
CHD1L
gene
expression
changes.
Here
we
apply
heuristic
fine-mapping
approach
to
prioritize
combinations
that
explain
majority
variance
identify
likely
driving
association.
We
assess
combined
impact
these
regulation
using
publicly
available
sequencing
studies,
test
relationship
between
imputed
from
monocytes.
Taken
together,
this
work
characterizes
genetically
regulated
further
expands
our
knowledge
CHD1L-mediated
restriction
Molecules,
Год журнала:
2023,
Номер
28(9), С. 3772 - 3772
Опубликована: Апрель 27, 2023
Pomegranate
(Punica
granatum
L.)
is
a
rich
source
of
polyphenols,
including
ellagitannins
and
ellagic
acid.
The
plant
used
in
traditional
medicine,
its
purified
components
can
provide
anti-inflammatory
antioxidant
activity
support
host
defenses
during
viral
infection
recovery
from
disease.
Current
data
show
that
pomegranate
polyphenol
extract
ellagitannin
metabolites
exert
their
beneficial
effects
by
controlling
immune
cell
infiltration,
regulating
the
cytokine
secretion
reactive
oxygen
nitrogen
species
production,
modulating
NFκB
pathway.
In
vitro,
extracts
interact
with
inhibit
infectivity
range
viruses,
SARS-CoV-2.
silico
docking
studies
bind
to
several
SARS-CoV-2
human
proteins,
number
proteases.
This
warrants
further
exploration
polyphenol–viral
polyphenol–host
interactions
vitro
vivo
studies.
extracts,
acid
are
promising
agents
target
virus
restrict
inflammatory
response
infections,
as
well
supplement
depleted
levels
stage
COVID-19.
Infectious Diseases and Therapy,
Год журнала:
2024,
Номер
13(1), С. 21 - 55
Опубликована: Янв. 1, 2024
In
1976
Ebola
revealed
itself
to
the
world,
marking
beginning
of
a
series
localized
outbreaks.
However,
it
was
outbreak
that
began
in
2013
incited
fear
and
anxiety
around
globe.
Since
then,
our
comprehension
virus
has
been
steadily
expanding.
(EBOV),
belonging
Orthoebolavirus
genus
Filoviridae
family,
possesses
non-segmented,
negative
single-stranded
RNA
genome
comprising
seven
genes
encode
multiple
proteins.
These
proteins
collectively
orchestrate
intricate
process
infecting
host
cells.
It
is
not
possible
view
each
protein
as
monofunctional.
Instead,
they
synergistically
contribute
pathogenicity
virus.
Understanding
this
multifaceted
replication
cycle
crucial
for
development
effective
antiviral
strategies.
Currently,
two
antibody-based
therapeutics
have
received
approval
treating
disease
(EVD).
2022,
first
evidence-based
clinical
practice
guideline
dedicated
specific
therapies
EVD
published.
Although
notable
progress
made
recent
years,
deaths
still
occur.
Consequently,
there
an
urgent
need
enhance
therapeutic
options
available
improve
outcomes
disease.
Emerging
can
target
viral
direct-acting
antivirals
or
factors
host-directed
antivirals.
They
both
advantages
disadvantages.
One
way
bypass
some
disadvantages
repurpose
already
approved
drugs
non-EVD
indications
treat
EVD.
This
review
offers
detailed
insight
into
role
virus,
understanding
how
interacts
with
cells
critical
emerging
exert
their
activity.
Using
knowledge,
delves
mechanisms
action
current
therapeutics.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 21, 2024
Abstract
In
the
realm
of
studying
joint-related
diseases,
there
is
a
continuous
quest
for
more
accurate
and
representative
models.
Recently,
regenerative
medicine
tissue
engineering
have
seen
growing
interest
in
utilizing
organoids
as
powerful
tools
complex
biological
systems
vitro.
Organoids,
three-dimensional
structures
replicating
architecture
function
organs,
provide
unique
platform
investigating
disease
mechanisms,
drug
responses,
regeneration.
The
surge
organoid
research
fueled
by
need
physiologically
relevant
models
to
bridge
gap
between
traditional
cell
cultures
vivo
studies.
Osteochondral
emerged
promising
avenue
this
pursuit,
offering
better
mimic
intricate
interactions
within
bone
cartilage.
This
review
explores
significance
osteochondral
their
development
advancing
our
understanding
treatment
cartilage-related
diseases.
It
summarizes
organoids’
insights
progress,
focusing
on
composition,
materials,
sources,
cultivation
methods,
well
concept
chips
application
scenarios.
Additionally,
we
address
limitations
challenges
these
face,
emphasizing
necessity
further
overcome
obstacles
facilitate
orthopedic
Mass Spectrometry Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 9, 2025
An
intricate
network
of
protein
assemblies
and
protein-protein
interactions
(PPIs)
underlies
nearly
every
biological
process
in
living
systems.
The
organization
these
cellular
networks
is
highly
dynamic
intimately
tied
to
the
genomic
proteomic
landscapes
a
cell.
Disruptions
normal
PPIs
can
impair
functions
contribute
development
human
diseases.
In
recent
years,
targeting
has
emerged
as
an
attractive
strategy
for
drug
discovery.
Consequently,
identification
characterization
endogenous
PPIs-those
occurring
naturally
under
physiological
conditions-has
become
crucial
unraveling
molecular
mechanisms
driving
pathology
laying
groundwork
novel
diagnostics
therapeutics.
Owing
numerous
technological
advancements,
mass
spectrometry
(MS)-based
proteomics
transformed
study
at
systems-level.
This
review
focuses
on
approaches
that
enable
physiologically
relevant
interactions,
spanning
complex-centric
structure-centric
analyses.
Additionally,
their
applications
define
native
contexts
cancer
viral
infectious
diseases
highlighted.
Emerging Microbes & Infections,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 19, 2025
Influenza
A
virus
(IAV)
is
a
significant
zoonotic
pathogen
that
poses
considerable
challenge
to
public
health
due
its
continuous
mutations.
Lipid
droplets
(LDs)
have
been
shown
play
an
important
role
in
the
process
of
several
viral
infections.
However,
their
IAV
infection
remains
unclear.
Here,
we
found
altered
lipid
metabolism
and
increased
content
LDs
lungs
mice.
In
vitro,
also
mediated
formation
A549
cells.
Besides,
inhibition
can
significantly
suppress
replication
release
inflammatory
factors,
indicating
could
facilitate
response.
Furthermore,
discovered
activate
SREBP2,
crucial
lipid-regulating
transcription
factor
regulates
expressions
downstream
proteins
named
HMGCR
HMGCS.
HMGCS
involved
cholesterol
synthesis,
which
further
promoted
LDs.
Additionally,
use
fatostatin
specifically
inhibits
maturation
SREBP2
was
able
H5N1
cells
effectively
ameliorated
IAV-induced
lung
injury
mice,
eventually
survival
rate
infected
Taken
together,
demonstrate
essential
roles
LD
pathogenesis,
may
better
advancement
new
strategies
against
infection,
especially
highly
pathogenic
virus.
