Nanozyme-enhanced tyramine signal amplification probe for preamplification-free myocarditis-related miRNAs detection
Chemical Engineering Journal,
Год журнала:
2024,
Номер
unknown, С. 158093 - 158093
Опубликована: Ноя. 1, 2024
Язык: Английский
MicroRNAs participate in the regulation of apoptosis and oxidative stress-related gene expression in rabbits infected with Lagovirus europaeus GI.1 and GI.2 genotypes
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Март 7, 2024
MicroRNAs
(miRs)
are
a
group
of
small,
17–25
nucleotide,
non-coding
RNA
that
regulate
gene
expression
at
the
post-transcriptional
level.
To
date,
little
is
known
about
molecular
signatures
regulatory
interactions
between
miRs
and
apoptosis
oxidative
stress
in
viral
diseases.
Lagovirus
europaeus
virus
causes
severe
disease
rabbits
(
Oryctolagus
cuniculus
)
called
Rabbit
Hemorrhagic
Disease
(RHD)
belongs
to
Caliciviridae
family,
genus.
Within
associated
with
RHD,
two
genotypes
(GI.1
GI.2)
have
been
distinguished,
GI.1
genotype
includes
four
variants
(GI.1a,
GI.1b,
GI.1c,
GI.1d).
The
study
aimed
assess
their
target
genes
involved
stress,
as
well
potential
impact
on
pathways
during
—two
infection
different
virulences
tissues
(liver,
lung,
kidneys,
spleen).
related
was
determined
using
quantitative
real-time
PCR
(qPCR).
In
this
study,
we
evaluated
miR-21
PTEN
,
PDCD4
),
miR-16b
Bcl-2
CXCL10
miR-34a
p53
SIRT1
miRs—related
stress—miR-122
Bach1
miR-132
Nfr-2
).
We
also
examined
biomarkers
both
processes
Bax
Bax/Bcl-2
ratio,
Caspase-3
PARP
HO-I
stress.
Our
report
first
present
effects
rabbit
lungs,
effect
indicates
that,
one
hand,
can
intensify
(miR-16b,
miR-34a)
organs
response
stimulus
and,
other
inhibit
(miR-21),
which
cases
may
be
determinant
pathogenesis
RHD
tissue
damage.
Biomarkers
/
ratio
promote
more
intense
after
GI.2
genotype.
findings
demonstrate
miR-122
HO-1
biomarker
course
hemorrhagic
show
miR-21,
miR-16b,
three
pathways.
Meanwhile,
Язык: Английский
ORF3a of SARS-CoV-2 modulates PI3K/AKT signaling in human lung epithelial cells via hsa-miR-155-5p
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
268, С. 131734 - 131734
Опубликована: Апрель 21, 2024
Язык: Английский
THE LABORATORY TESTING OF THE PCR-BASED PROTOCOL OF DETECTION OF THE RABBIT HAEMORRHAGIC DISEASE VIRUS RNA
One Health Journal,
Год журнала:
2024,
Номер
2(III), С. 39 - 44
Опубликована: Июль 1, 2024
Rabbit
Haemorrhagic
Disease
(RHD),
also
known
as
Viral
(RVHD),
is
a
highly
contagious
and
often
fatal
viral
disease
that
affects
domestic
wild
rabbits.
It's
caused
by
two
related
viruses:
Virus
(RHDV)
2
(RHDV2).
The
endemic
in
many
European,
Asian
American
countries,
but
the
agent
still
recognized
an
emergent
infection,
associated
with
mass
losses
extremely
high
mortality
rabbits
of
all
breeds.
One
Health
Scientific
Research
Institute,
PSI
collaboration
SRI
‘Veterinary
Biotechnologies’,
LLC
developed
house
PCR-based
protocol
for
RHDV
detection
RHDV-2
differentiation,
requires
fast
implementation.
This
diagnostics
kit
evaluation
described
under
OIE
requirements
determination
sensitivity,
specificity,
repeatability
domain-specificity.
Detection
recommended
practical
application.
Язык: Английский
MicroRNAs Regulate the Expression of Genes Related to the Innate Immune and Inflammatory Response in Rabbits Infected with Lagovirus europaeus GI.1 and GI.2 Genotypes
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(17), С. 9531 - 9531
Опубликована: Сен. 2, 2024
MicroRNAs
(miR)
are
a
group
of
small,
non-coding
RNAs
17-25
nucleotides
that
regulate
gene
expression
at
the
post-transcriptional
level.
Dysregulation
miRNA
or
function
may
contribute
to
abnormal
and
signaling
pathways,
leading
disease
pathology.
Lagovirus
europaeus
(L.
europaeus)
causes
severe
in
rabbits
called
rabbit
hemorrhagic
(RHD).
The
symptoms
liver,
lung,
kidney,
spleen
degeneration
observed
during
RHD
similar
those
acute
liver
failure
(ALF)
multi-organ
(MOF)
humans.
In
this
study,
we
assessed
miRs
their
target
genes
involved
innate
immune
inflammatory
response.
Also,
potential
impact
on
pathways
L.
infection-two
genotypes
(GI.1
GI.2)-in
lungs,
kidneys,
spleen.
was
determined
using
quantitative
real-time
PCR
(qPCR).
We
miR-155
(MyD88,
TAB2,
p65,
NLRP3),
miR-146a
(IRAK1,
TRAF6),
miR-223
(TLR4,
IKKα,
miR-125b
(MyD88).
also
examined
biomarkers
inflammation:
IL-1β,
IL-6,
TNF-α,
IL-18
four
tissues
mRNA
Our
study
shows
main
regulators
response
europaeus/GI.1
GI.2
infection,
as
well
RHD,
miR-155,
miR-223,
miR-146a.
During
infection
with
europaeus/RHD,
has
both
pro-
anti-inflammatory
effects
kidneys
spleen;
lungs
kidneys;
all
tissues;
however,
only
liver.
each
case,
such
an
effect
be
determinant
pathogenesis
RHD.
research
three
infection.
However,
result
regulation
influenced
by
tissue
microenvironment.
overexpression
two
critical
phase
cytokines:
IL-6
TNF-α
(in
spleen).
IL-1β
highly
expressed
after
These
facts
indicate
strong
rapid
involvement
local
GI.2)-and
Profile
inflammation
infected
regarding
nature
changes
but
different
for
individual
tissues.
Therefore,
propose
profiles
GI.1
(pulmonary,
renal,
Язык: Английский
Digital PCR in Virology: Current Applications and Future Perspectives
Molecular Diagnosis & Therapy,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 2, 2024
Язык: Английский
Epigenetic alterations of miR-155 and global DNA methylation as potential mediators of ochratoxin A cytotoxicity and carcinogenicity in human lung fibroblasts
Environmental Science and Pollution Research,
Год журнала:
2023,
Номер
31(4), С. 5473 - 5483
Опубликована: Дек. 20, 2023
Abstract
Ochratoxin
A
(OTA)
is
a
well-known
mycotoxin
that
adversely
affects
different
human
cells.
Inhalational
exposure
to
OTA
and
subsequent
pulmonary
diseases
have
been
previously
reported,
yet
its
potential
carcinogenicity
underlying
molecular
mechanisms
not
fully
elucidated.
This
study
aimed
evaluate
the
OTA-induced
cytotoxicity
epigenetic
changes
in
fetal
lung
fibroblast
(WI-38)
was
assessed
by
MTT
assay;
RT-qPCR
used
determine
expression
of
BAX
,
BCL-2
TP53
miR-155
while
ELISA
for
measuring
5-methyl
cytosine
percentage
assess
global
DNA
methylation
OTA-treated
versus
control
WI-38
cells
demonstrated
sensitivity
with
IC50
at
22.38
μM.
Though
Bcl-2
were
downregulated,
low
/
ratio,
upregulated,
their
fold
showed
decline
trend
increasing
concentration.
significant
dose-dependent
upregulation
observed,
dynamic
time-related
decline.
Using
subtoxic
concentrations,
hypermethylation
alterations
identified.
Global
are
mediate
toxicity
on
downregulation,
reduced
ratio
together
indicated
either
inhibition
-dependent
apoptosis
or
tissue
specific
response
exposure.
The
aforementioned
variations
present
new
evidence
possible
Graphical
Язык: Английский