Mitochondrial morphodynamics are modulated by physiological range of temperature and influence host cell outcomes during influenza infection DOI Creative Commons

Grant J. McLoughlin,

Andrew Pekosz

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 6, 2024

Abstract Influenza viruses replicate in both the cooler, upper portions of airway and warmer, lower respiratory tract. This study investigates how physiological ranges temperature, specifically 33°C 37°C, impact host cell biology temperature-dependent differences cells influence outcomes during influenza A virus infection. prioritizes describing mitochondrial networks due to their importance maintaining cellular homeostasis mediating immune responses viral The temperature at which are incubated significantly influences network morphology function. Moreover, changes prior infection result temperature-specific These findings indicate that structure alone can modulate form function mitochondria directly production. While not all processes were shown be affected by or infection, these results highlight using physiologically relevant temperatures pathogen research elucidate dynamics contribute Importance Respiratory infect tract but rarely is (33°C 37°C) considered. Mitochondria central mediators numerous pathways, functions often modified Physiological alter function, further impacted sheds light on concert with

Язык: Английский

ROS Induced by Aphrocallistes vastus Lectin Enhance Oncolytic Vaccinia Virus Replication and Induce Apoptosis in Hepatocellular Carcinoma Cells DOI Creative Commons
Yanan Zhang, Ying Zhu,

Gaohui Jiang

и другие.

Marine Drugs, Год журнала: 2024, Номер 22(7), С. 307 - 307

Опубликована: Июнь 30, 2024

Oncolytic virotherapy is expected to provide a new treatment strategy for cancer. Aphrocallistes vastus lectin (AVL) Ca2+-dependent receptor containing the conserved domain of C-type and hydrophobic N-terminal region, which can bind bird’s nest glycoprotein D-galactose. Our previous studies suggested that oncolytic vaccinia virus (oncoVV) armed with AVL gene exerted remarkable replication antitumor effects in vitro vivo. In this study, we found oncoVV-AVL may reprogram metabolism hepatocellular carcinoma cells promote ROS, elevated ROS subsequently promoted viral induced apoptosis. This study will theoretical basis application liver

Язык: Английский

Процитировано

4
A mitochondrion-targeted poly(N-isopropylacrylamide-coacrylic acid) nanohydrogel with a fluorescent bioprobe for ferrous ion imaging in vitro and in vivo DOI
Wenlong Xiao, Fang Wang, Xuchen Wang

и другие.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер 330, С. 125725 - 125725

Опубликована: Янв. 9, 2025

Язык: Английский

Процитировано

0
scRNA seq of an F1 cross of Marek’s disease resistant and susceptible chickens identifies allele specific expression signatures enriched in transcription modulators DOI Creative Commons
Deborah Velez‐Irizarry, Hans H. Cheng, C. J. Hearn

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 29, 2025

Язык: Английский

Процитировано

0
Enterovirus A71-induced glycolysis is essential for viral replication by activating PI3K/Akt pathway DOI

Li Dan,

Jing Yang, Yijun Shen

и другие.

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107505 - 107505

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Mitochondria-dependent innate immunity: A potential therapeutic target in Flavivirus infection DOI

Saurabh Losarwar,

Bhaskaranand Pancholi, R. Ramesh Babu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 154, С. 114551 - 114551

Опубликована: Март 31, 2025

Язык: Английский

Процитировано

0
Dynamic Molecular Changes in Brain, Lung, and Heart of Hamsters Infected With SARS‐CoV‐2: Insights From a Severe and Recovery Phase Model DOI
Wanjun Peng, Xiaohui Wei, Yue Wu

и другие.

Journal of Medical Virology, Год журнала: 2025, Номер 97(6)

Опубликована: Май 27, 2025

ABSTRACT The Global pandemic of coronavirus disease 2019 was initiated by the emergence severe acute respiratory syndrome 2. In addition to conventional pulmonary lesions, a range neurological injury symptoms have been identified in clinical practice, but aetiology disorders linked SARS‐CoV‐2 infection remains poorly understood. Syrian hamsters, which are highly susceptible infection, exhibit phenotype similar that observed human COVID‐19 patients. this study, hamster model used analyze molecular changes different tissues at various time points post with distinct strains using proteomic and phosphoproteomic approaches. Multi‐omics analysis showed SARS‐COV‐2 triggers sustained downregulation abundance phosphorylation levels neuronal synapse‐associated proteins brain, suggesting damage persists even during recovery period. Additionally, infections may contribute onset long‐term impacting energy metabolism, neurotransmitter release, synaptic transmission pathways. This study provides comprehensive profile hamsters infected tissues, offering foundational insights into pathogenic mechanisms COVID‐19.

Язык: Английский

Процитировано

0
Improvement on mitochondrial energy metabolism of Codonopsis pilosula (Franch.) Nannf. polysaccharide DOI Creative Commons
He Li, Letian Zhang,

Xing-Tai Li

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 30, 2025

Ethnopharmacological relevance Codonopsis pilosula (Franch.) Nannf. (CP) is one of the most popular Qi-invigorating herbal medicines and has been extensively used to promote health vitality in China for a long time. polysaccharide (CPP) principal active components CP, which considered as reason CP widespread application. However, it not revealed that CPP exert Qi-invigoration effect by protecting mitochondria and/or improving mitochondrial function existing traditional Chinese medicine theories. Aim study We extracted from C. investigated effects on energy metabolism protection. Methods Based mice chronic hypoxia model imitating deficiency state human body, was administered with oral gavage daily 10 days, permeability transition (MPT), lipid peroxidation product malondialdehyde (MDA) brain, respiratory function, levels adenosine triphosphate (ATP), diphosphate (ADP) monophosphate (AMP) liver cells were assayed. Adenylate charge (AEC), total adenylate pool (TAP), ATP/ADP, ATP/AMP ratios calculated. Results can inhibit formation MDA brains, decrease oxygen consuming rate control ratio (RCR) mitochondria, increase ATP, TAP AEC under condition. Conclusion possess improve bioenergetic levels.

Язык: Английский

Процитировано

0
Physiological Adaptation of Fenneropenaeus chinensis in Response to Saline–Alkaline Stress Revealed by a Combined Proteomics and Metabolomics Method DOI Creative Commons

Tian Gao,

Qiong Wang,

Huarui Sun

и другие.

Biology, Год журнала: 2024, Номер 13(7), С. 488 - 488

Опубликована: Июнь 30, 2024

The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize abundant inland saline-alkaline water, we studied physiological effects high carbonate alkalinity stress and pH on

Язык: Английский

Процитировано

2
Immunometabolites in viral infections: Action mechanism and function DOI
Behnaz Bouzari,

Uliana Y. Chugaeva,

Sajad Karampoor

и другие.

Journal of Medical Virology, Год журнала: 2024, Номер 96(7)

Опубликована: Июль 1, 2024

Abstract The interplay between viral pathogens and host metabolism plays a pivotal role in determining the outcome of infections. Upon detection, metabolic landscape cell undergoes significant changes, shifting from oxidative respiration via tricarboxylic acid (TCA) cycle to increased aerobic glycolysis. This shift is accompanied by elevated nutrient accessibility, which vital for function, development, proliferation. Furthermore, depositing metabolites derived fatty acids, TCA intermediates, amino catabolism accelerates immunometabolic transition, facilitating pro‐inflammatory antimicrobial responses. Immunometabolites refer small molecules involved cellular regulating immune response. These include nutrients, such as glucose along with intermediates signaling adenosine, lactate, itaconate, succinate, kynurenine, prostaglandins. Emerging evidence suggests that immunometabolites released cells establish complex interaction network within local niches, orchestrating fine‐tuning responses during diseases. However, our current understanding immense capacity convey essential signals one another or compartments remains incomplete. Unraveling these complexities would be crucial harnessing potential therapeutic interventions. In this review, we discuss specific their mechanisms action infections, emphasizing recent findings future directions rapidly evolving field.

Язык: Английский

Процитировано

1
Mitochondrial resilience and antioxidant defence against HIV-1: unveiling the power of Asparagus racemosus extracts and Shatavarin IV DOI Creative Commons
Pratiksha Jadaun,

R Harshithkumar,

Chandrabhan Seniya

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Окт. 23, 2024

Asparagus racemosus (AR), an Ayurvedic botanical, possesses various biological characteristics, yet its impact on HIV-1 replication remains to be elucidated. This study aimed investigate the inhibitory effects of AR root extracts and principal bioactive molecule, Shatavarin IV (Shatavarin), their role in mitigating mitochondrial dysfunction during infection, utilizing both vitro silico methodologies. The cytotoxicity was evaluated using MTT ATPlite assays. In anti-HIV-1 activity assessed TZM-bl cells against X4 R5 subtypes, confirmed peripheral blood mononuclear p24 antigen capture ELISA viral copy number assessment. Mechanistic insights were obtained through enzymatic assays targeting Integrase, Protease Reverse Transcriptase. Shatavarin’s also validated via assays, along with molecular interaction studies key enzymes. induced by detecting reactive oxygen species (ROS), calcium accumulation, potential, caspase within infected cells. Non-cytotoxic concentrations aqueous hydroalcoholic derived from roots displayed dose-dependent inhibition replication. Notably, extract exhibited superior Transcriptase activity, complemented moderate observed assay. Molecular revealed that IV, constituent AR, formed hydrogen bonds active binding pocket site residues crucial for HIV enzyme catalysis, suggesting potential attenuating infection. Mitochondrial marked increased oxidative stress, overload, loss membrane elevated effectively mitigated treatment IV. These findings underscore as antiviral agents, while enhancing function conclusion, extracts, particularly demonstrate promising concurrently ameliorating virus. suggest therapeutic combating infection improving health.

Язык: Английский

Процитировано

1