Mitochondrial morphodynamics are modulated by physiological range of temperature and influence host cell outcomes during influenza infection DOI Creative Commons

Grant J. McLoughlin,

Andrew Pekosz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Influenza viruses replicate in both the cooler, upper portions of airway and warmer, lower respiratory tract. This study investigates how physiological ranges temperature, specifically 33°C 37°C, impact host cell biology temperature-dependent differences cells influence outcomes during influenza A virus infection. prioritizes describing mitochondrial networks due to their importance maintaining cellular homeostasis mediating immune responses viral The temperature at which are incubated significantly influences network morphology function. Moreover, changes prior infection result temperature-specific These findings indicate that structure alone can modulate form function mitochondria directly production. While not all processes were shown be affected by or infection, these results highlight using physiologically relevant temperatures pathogen research elucidate dynamics contribute Importance Respiratory infect tract but rarely is (33°C 37°C) considered. Mitochondria central mediators numerous pathways, functions often modified Physiological alter function, further impacted sheds light on concert with

Language: Английский

ROS Induced by Aphrocallistes vastus Lectin Enhance Oncolytic Vaccinia Virus Replication and Induce Apoptosis in Hepatocellular Carcinoma Cells DOI Creative Commons
Yanan Zhang, Ying Zhu,

Gaohui Jiang

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 307 - 307

Published: June 30, 2024

Oncolytic virotherapy is expected to provide a new treatment strategy for cancer. Aphrocallistes vastus lectin (AVL) Ca2+-dependent receptor containing the conserved domain of C-type and hydrophobic N-terminal region, which can bind bird’s nest glycoprotein D-galactose. Our previous studies suggested that oncolytic vaccinia virus (oncoVV) armed with AVL gene exerted remarkable replication antitumor effects in vitro vivo. In this study, we found oncoVV-AVL may reprogram metabolism hepatocellular carcinoma cells promote ROS, elevated ROS subsequently promoted viral induced apoptosis. This study will theoretical basis application liver

Language: Английский

Citations

4
Mitochondria-dependent innate immunity: A potential therapeutic target in Flavivirus infection DOI

Saurabh Losarwar,

Bhaskaranand Pancholi, R. Ramesh Babu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 154, P. 114551 - 114551

Published: March 31, 2025

Language: Английский

Citations

0
A mitochondrion-targeted poly(N-isopropylacrylamide-coacrylic acid) nanohydrogel with a fluorescent bioprobe for ferrous ion imaging in vitro and in vivo DOI
Wenlong Xiao, Fang Wang, Xuchen Wang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 330, P. 125725 - 125725

Published: Jan. 9, 2025

Language: Английский

Citations

0
scRNA seq of an F1 cross of Marek’s disease resistant and susceptible chickens identifies allele specific expression signatures enriched in transcription modulators DOI Creative Commons
Deborah Velez‐Irizarry, Hans H. Cheng, C. J. Hearn

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

Language: Английский

Citations

0
Enterovirus A71-induced glycolysis is essential for viral replication by activating PI3K/Akt pathway DOI

Li Dan,

Jing Yang, Yijun Shen

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107505 - 107505

Published: March 1, 2025

Language: Английский

Citations

0
Mitochondrial resilience and antioxidant defence against HIV-1: unveiling the power of Asparagus racemosus extracts and Shatavarin IV DOI Creative Commons
Pratiksha Jadaun,

R Harshithkumar,

Chandrabhan Seniya

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Asparagus racemosus (AR), an Ayurvedic botanical, possesses various biological characteristics, yet its impact on HIV-1 replication remains to be elucidated. This study aimed investigate the inhibitory effects of AR root extracts and principal bioactive molecule, Shatavarin IV (Shatavarin), their role in mitigating mitochondrial dysfunction during infection, utilizing both vitro silico methodologies. The cytotoxicity was evaluated using MTT ATPlite assays. In anti-HIV-1 activity assessed TZM-bl cells against X4 R5 subtypes, confirmed peripheral blood mononuclear p24 antigen capture ELISA viral copy number assessment. Mechanistic insights were obtained through enzymatic assays targeting Integrase, Protease Reverse Transcriptase. Shatavarin’s also validated via assays, along with molecular interaction studies key enzymes. induced by detecting reactive oxygen species (ROS), calcium accumulation, potential, caspase within infected cells. Non-cytotoxic concentrations aqueous hydroalcoholic derived from roots displayed dose-dependent inhibition replication. Notably, extract exhibited superior Transcriptase activity, complemented moderate observed assay. Molecular revealed that IV, constituent AR, formed hydrogen bonds active binding pocket site residues crucial for HIV enzyme catalysis, suggesting potential attenuating infection. Mitochondrial marked increased oxidative stress, overload, loss membrane elevated effectively mitigated treatment IV. These findings underscore as antiviral agents, while enhancing function conclusion, extracts, particularly demonstrate promising concurrently ameliorating virus. suggest therapeutic combating infection improving health.

Language: Английский

Citations

1
Physiological Adaptation of Fenneropenaeus chinensis in Response to Saline–Alkaline Stress Revealed by a Combined Proteomics and Metabolomics Method DOI Creative Commons

Tian Gao,

Qiong Wang,

Huarui Sun

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 488 - 488

Published: June 30, 2024

The rapid development of the mariculture industry has been hindered by limited coastal aquaculture space. To utilize abundant inland saline-alkaline water, we studied physiological effects high carbonate alkalinity stress and pH on

Language: Английский

Citations

1
Immunometabolites in viral infections: Action mechanism and function DOI
Behnaz Bouzari,

Uliana Y. Chugaeva,

Sajad Karampoor

et al.

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(7)

Published: July 1, 2024

Abstract The interplay between viral pathogens and host metabolism plays a pivotal role in determining the outcome of infections. Upon detection, metabolic landscape cell undergoes significant changes, shifting from oxidative respiration via tricarboxylic acid (TCA) cycle to increased aerobic glycolysis. This shift is accompanied by elevated nutrient accessibility, which vital for function, development, proliferation. Furthermore, depositing metabolites derived fatty acids, TCA intermediates, amino catabolism accelerates immunometabolic transition, facilitating pro‐inflammatory antimicrobial responses. Immunometabolites refer small molecules involved cellular regulating immune response. These include nutrients, such as glucose along with intermediates signaling adenosine, lactate, itaconate, succinate, kynurenine, prostaglandins. Emerging evidence suggests that immunometabolites released cells establish complex interaction network within local niches, orchestrating fine‐tuning responses during diseases. However, our current understanding immense capacity convey essential signals one another or compartments remains incomplete. Unraveling these complexities would be crucial harnessing potential therapeutic interventions. In this review, we discuss specific their mechanisms action infections, emphasizing recent findings future directions rapidly evolving field.

Language: Английский

Citations

1
Mitochondrial DNA Missense Mutations ChrMT: 8981A > G and ChrMT: 6268C > T Identified in a Caucasian Female with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) Triggered by the Epstein–Barr Virus DOI Creative Commons
Gaoyan G. Tang‐Siegel,

David W. Maughan,

Milah B. Frownfelter

et al.

Case Reports in Genetics, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 10

Published: May 9, 2024

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multisystem disabling disease with unclear etiology and pathophysiology, whose typical symptoms include prolonged debilitating recovery from or postexertional malaise (PEM). Disrupted production of adenosine triphosphate (ATP), the intracellular energy that fuels cellular activity, cause for fatigue. Here, we present long-term case ME/CFS: 75-year-old Caucasian female patient, ME/CFS were clearly triggered by an acute infection Epstein–Barr virus 24 years ago (mononucleosis). Before then, patient was healthy professional woman. A recent DNA sequence analysis identified missense variants mitochondrial respiratory chain enzymes, including ATP6 (ChrMT: 8981A > G; Q152R) Cox1 6268C T; A122V). Protein subunits are encoded outside nucleus: gene encodes subunit 1 complex IV (CIV: cytochrome c oxidase) V (CV: ATP synthase). CIV CV last two five essential enzymes perform electron transport reaction to generate ATP. Further blood sample using transmission microscopy demonstrated abnormal, circulating, extracellular mitochondria. These results indicate had dysfunctional mitochondria, which may contribute directly her major symptoms, PEM neurological cognitive changes. Furthermore, A122V), functioning at later stage production, play role in abnormality patient’s mitochondria development symptoms.

Language: Английский

Citations

0
Mitochondrial morphodynamics are modulated by physiological range of temperature and influence host cell outcomes during influenza infection DOI Creative Commons

Grant J. McLoughlin,

Andrew Pekosz

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Influenza viruses replicate in both the cooler, upper portions of airway and warmer, lower respiratory tract. This study investigates how physiological ranges temperature, specifically 33°C 37°C, impact host cell biology temperature-dependent differences cells influence outcomes during influenza A virus infection. prioritizes describing mitochondrial networks due to their importance maintaining cellular homeostasis mediating immune responses viral The temperature at which are incubated significantly influences network morphology function. Moreover, changes prior infection result temperature-specific These findings indicate that structure alone can modulate form function mitochondria directly production. While not all processes were shown be affected by or infection, these results highlight using physiologically relevant temperatures pathogen research elucidate dynamics contribute Importance Respiratory infect tract but rarely is (33°C 37°C) considered. Mitochondria central mediators numerous pathways, functions often modified Physiological alter function, further impacted sheds light on concert with

Language: Английский

Citations

0