Potential of a Bead-Based Multiplex Assay for SARS-CoV-2 Antibody Detection DOI Creative Commons

Karla Rottmayer,

Mandy Schwarze,

Christian Jassoy

и другие.

Biology, Год журнала: 2024, Номер 13(4), С. 273 - 273

Опубликована: Апрель 18, 2024

Serological assays for SARS-CoV-2 play a pivotal role in the definition of whether patients are infected, understanding viral epidemiology, screening convalescent sera therapeutic and prophylactic purposes, obtaining better immune response towards virus. The aim this study was to investigate performance bead-based multiplex assay. This assay allowed simultaneous testing IgG antibodies against spike, S1, S2, RBD, nucleocapsid moieties S1 seasonal coronaviruses hCoV-22E, hCoV-HKU1, hCoV-NL63, hCoV-OC43, as well MERS SARS-CoV. We compared with commercial ELISA tests. tested 27 PCR-positive individuals who were previously different assays. Additionally, we investigated reproducibility results by means multiple same sera. Finally, correlated neutralising In summary, concordance qualitative ranged between 78% 96% depending on specific antigen. Repeated freezing-thawing cycles resulted reduced mean fluorescence intensity, while storage period had no influence respect. our test cohort, detected up 36% positive development antibodies, which is ELISA.

Язык: Английский

NVX-CoV2373 induces humoral and cellular immune responses that are functionally comparable to vector and mRNA-based vaccines DOI Creative Commons
Franz Mai, Marcel Kordt, Wendy Bergmann‐Ewert

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Март 18, 2024

Background After licensing of the protein-based vaccine NVX-CoV2373, three technically different vaccines against SARS-CoV-2 became available for application to human population - and comparison efficacies. Methods We here recruited 42 study participants who had obtained one initial dose NVX-CoV2373 analyzed their immune responses in contrast 37 either vector AZD1222 or mRNA BNT162b2 a year earlier. 32 also donated blood before first vaccination serve as vaccine-naive control. In detail, we investigated quantified at day 21 approximately six months after primary immunization amounts vaccine-specific antibodies produced, neutralization capacity, quality terms binding epitopes efficiency inducing various isotypes. Cellular immunity intracellular cytokine production following vitro re-stimulation with was via ELISpot flow cytometry. Results Our results show that even though including yielded best almost any aspect antibody levels efficiency, capacities wild-type Wuhan strain Omicron BA.1 variant early were comparable among all groups. As T cells, observed prevailing CD8 response weeks which turned into predominant CD4 memory has not yet been BNT162b2. While additional infection resulted boost humoral response, cell appeared rather unaffected. Conclusion Whether these differences translate real world protection from infection, mitigation severe disease courses prevention long/post COVID will need be future.

Язык: Английский

Процитировано

4

Editorial for SARS-CoV-2 and COVID-19 Topical Collection DOI Creative Commons
Luis Martínez‐Sobrido, Fernando Almazán

Viruses, Год журнала: 2024, Номер 16(3), С. 356 - 356

Опубликована: Фев. 25, 2024

A previously unknown coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was isolated in Wuhan, China December 2019, from a patient with disease linked to potential contact wild animals [...]

Язык: Английский

Процитировано

1

Potential of a Bead-Based Multiplex Assay for SARS-CoV-2 Antibody Detection DOI Creative Commons

Karla Rottmayer,

Mandy Schwarze,

Christian Jassoy

и другие.

Biology, Год журнала: 2024, Номер 13(4), С. 273 - 273

Опубликована: Апрель 18, 2024

Serological assays for SARS-CoV-2 play a pivotal role in the definition of whether patients are infected, understanding viral epidemiology, screening convalescent sera therapeutic and prophylactic purposes, obtaining better immune response towards virus. The aim this study was to investigate performance bead-based multiplex assay. This assay allowed simultaneous testing IgG antibodies against spike, S1, S2, RBD, nucleocapsid moieties S1 seasonal coronaviruses hCoV-22E, hCoV-HKU1, hCoV-NL63, hCoV-OC43, as well MERS SARS-CoV. We compared with commercial ELISA tests. tested 27 PCR-positive individuals who were previously different assays. Additionally, we investigated reproducibility results by means multiple same sera. Finally, correlated neutralising In summary, concordance qualitative ranged between 78% 96% depending on specific antigen. Repeated freezing-thawing cycles resulted reduced mean fluorescence intensity, while storage period had no influence respect. our test cohort, detected up 36% positive development antibodies, which is ELISA.

Язык: Английский

Процитировано

0