Integrated hepatitis e virus monitoring in central Argentina: a six-year analysis of clinical surveillance and wastewater-based epidemiology
Water Research,
Год журнала:
2024,
Номер
261, С. 122004 - 122004
Опубликована: Июнь 27, 2024
Язык: Английский
Protocol for Detection of pathogenic enteric RNA viruses by regular monitoring of environmental samples from wastewater treatment plants using Droplet Digital PCR
Science in One Health,
Год журнала:
2024,
Номер
3, С. 100080 - 100080
Опубликована: Янв. 1, 2024
The
present
comprehensive
protocol
is
focused
on
the
detection
of
pathogenic
enteric
RNA
viruses,
explicitly
focusing
norovirus
genogroup
Ⅱ
(GⅡ),
astrovirus,
rotavirus,
Aichi
virus,
sapovirus,
hepatitis
A
and
E
viruses
in
wastewater
treatment
plants
through
droplet
digital
PCR
(ddPCR).
Enteric
are
significant
public
health
concern,
as
they
leading
cause
diseases
like
gastroenteritis.
Regular
monitoring
environmental
samples,
particularly
from
plants,
crucial
for
early
control
these
viruses.
This
research
aims
to
improve
understanding
prevalence
dynamics
urban
India
will
serve
a
model
similar
studies
other
regions.
Our
protocol's
objective
establish
novel
ddPCR-based
methodology
molecular
characterization
samples
sourced
Bhopal,
India.
assay
capable
accurately
quantifying
virus
concentrations
without
standard
curves,
minimizing
extensive
optimization,
enhancing
sensitivity
precision,
especially
low-abundance
targets.
Язык: Английский
Wastewater quality during the COVID-19 pandemic: a retrospective analysis of a Polish case study
International Journal of Environmental Science and Technology,
Год журнала:
2024,
Номер
22(6), С. 4125 - 4142
Опубликована: Авг. 20, 2024
Abstract
The
global
crisis
of
the
Coronavirus
disease-2019
(COVID-19)
pandemic
has
caused
an
enormous
burden
not
only
on
health,
social,
and
economic
sectors
but
also
many
essential
municipal
services.
Water
distribution
wastewater
management
have
faced
increasing
uncertainty
due
to
possible
transmission
Severe
Acute
Respiratory
Syndrome
2
(SARS-CoV-2)
via
aerosols.
Additionally,
behavioral
social
changes
impacted
water/wastewater
services
need
for
adaptation.
In
this
study,
presence
SARS-CoV-2
in
influent
treatment
plant
located
Poland
was
analyzed
November–December
2020,
while
other
parameters
important
sector
(e.g.:
water
usage
quality)
were
monitored
long-term
period
(2015–2020)
during
working
days,
holidays,
including
2020
lockdowns.
all
tested
samples,
genetic
material
detected,
which
confirmed
utility
monitoring
analyse
COVID-19
trends
local
communities.
Another
issue
elevated
surfactants
observed
change
organic
matter
structure
raw
generated
Also
NGS
approach
16S
rRNA
amplicon
sequencing
functional
diversity
heterogeneity
activated
sludge
bacterial
community,
especially
terms
nutrients
removal
biomass
separation.
Thus,
it
is
crucial
a
baseline
understanding
how
water-wastewater
can
serve
control
disease
among
community
tools
should
be
adopted
as
countermeasures
new
consumption
patterns
keeping
safe
effective
treatment.
Graphical
abstract
Язык: Английский
Wastewater-based surveillance of respiratory viruses in Northern Tuscany (Italy): Challenges and added value for public health purposes
The Science of The Total Environment,
Год журнала:
2024,
Номер
957, С. 177752 - 177752
Опубликована: Ноя. 30, 2024
During
the
COVID-19
pandemic,
wastewater-based
surveillance
(WBS)
showed
great
potential
as
an
early
warning
system
and
could
complement
human
clinical
surveillance.
This
study
aimed
to
highlight
added
value
of
WBS
for
respiratory
infections
alongside
different
systems.
Sewage
collected
at
entry
four
Wastewater
Treatment
Plants
in
Northern
Tuscany
(Italy)
were
analyzed
SARS-CoV-2,
Human
Adenovirus
(HAdV),
Respiratory
Syncytial
Virus
(RSV)
Influenza
(IV),
over
two
years.
Clinical
data
available
area,
while
other
viruses
came
from
national
virological
For
correlation
was
highly
significant
between
hospitalization
(ρ
=
0.8460),
but
not
wastewater
or
0.1682
ρ
0.0569,
respectively).
SARS-CoV-2
RNA
found
even
period
when
cases
reported,
indicating
a
continuous
community
circulation.
HAdVs
detected
74.3
%
samples,
most
sequences
identified
belonged
enteric
species
(HAdV-F41),
need
distinguishing
causing
diseases
RSV
only
winter
2022-2023,
IV
had
been
wastewater,
probably
due
poor
test
sensitivity.
In
conclusion,
although
there
may
be
various
challenges
testing
targets,
can
provide
pathogen-specific
situational
assessment
which
complements
existing
Язык: Английский