Recent developments and future directions in point-of-care next-generation CRISPR-based rapid diagnosis
Youssef Mohamed Hassan,
Ahmed Mohamed,
Yahaya Hassan
и другие.
Clinical and Experimental Medicine,
Год журнала:
2025,
Номер
25(1)
Опубликована: Янв. 9, 2025
Язык: Английский
H9N2 avian influenza virus diagnostics utilizing specific high-sensitivity enzymatic molecular system termed RPA-based CRISPR-Cas13a
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 140474 - 140474
Опубликована: Янв. 1, 2025
Язык: Английский
Prevention and control of avian influenza virus: Recent advances in diagnostic technologies and surveillance strategies
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 15, 2025
Язык: Английский
A Rapid Visual Detection Method for Sugarcane Streak Mosaic Virus Based on One-tube RPA-CRISPR/Cas12a
Talanta,
Год журнала:
2025,
Номер
291, С. 127888 - 127888
Опубликована: Март 3, 2025
Язык: Английский
A lateral flow biosensor based on duplex-specific nuclease and novel isothermal amplification for detection of influenza virus
Enzyme and Microbial Technology,
Год журнала:
2025,
Номер
188, С. 110653 - 110653
Опубликована: Апрель 8, 2025
Язык: Английский
Application of CRISPR/Cas gene editing for infectious disease control in poultry
Open Life Sciences,
Год журнала:
2025,
Номер
20(1)
Опубликована: Янв. 1, 2025
Abstract
The
poultry
industry
faces
multifaceted
challenges,
including
escalating
demand
for
products,
climate
change
impacting
feed
availability,
emergence
of
novel
avian
pathogens,
and
antimicrobial
resistance.
Traditional
disease
control
measures
are
costly
not
always
effective,
prompting
the
need
complementary
methods.
Gene
editing
(GE,
also
called
genome
editing)
technologies,
particularly
CRISPR/Cas9,
offer
promising
solutions.
This
article
summarizes
recent
advancements
in
utilizing
CRISPR/Cas
GE
to
enhance
infectious
poultry.
It
begins
with
an
overview
modern
techniques,
highlighting
CRISPR/Cas9’s
advantages
over
other
potential
applications
prevention
explored,
engineering
innovative
vaccines,
generation
disease-resilient
birds,
vivo
pathogen
targeting.
Additionally,
insights
provided
regarding
regulatory
frameworks
future
perspectives
this
rapidly
evolving
field.
Язык: Английский
CRISPR/Cas system and its application in the diagnosis of animal infectious diseases
The FASEB Journal,
Год журнала:
2024,
Номер
38(24)
Опубликована: Дек. 13, 2024
Abstract
Infectious
diseases
are
a
serious
threat
to
the
existence
of
animals
and
humans'
life.
In
21st
century,
emergence
re‐emergence
several
zoonotic
non‐zoonotic
global
pandemic
socio‐economic
importance
has
affected
billions
humans
animals.
The
need
for
expensive
equipment
laboratories,
non‐availability
on‐site
testing
abilities,
with
time‐consuming
low
sensitivity
specificity
issues
currently
available
diagnostic
techniques
identify
these
pathogenic
micro‐organisms
on
large
scale
highlighted
developing
cheap,
portable
environment
friendly
methods.
recent
years,
have
been
addressed
by
clustered
regularly
interspaced
palindromic
repeats
(CRISPR)‐based
platforms
that
transformed
molecular
field
due
their
outstanding
ultra‐sensitive
nucleic
acid
detecting
capabilities.
this
study,
we
highlight
types,
potential
different
Cas
proteins,
amplification
systems.
We
also
illuminate
application
CRISPR
integrated
setups
diagnosis
infectious
diseases,
majorly
in
food‐producing
(pigs,
ruminants,
poultry,
aquaculture),
domestic
pets
(dogs
cats),
importance.
conclude
challenges
future
perspectives
using
systems
rapidly
diagnose
treat
other
develop
control
strategies
prevent
spread
organisms.
Язык: Английский
A call to innovate Antarctic avian influenza surveillance
Trends in Ecology & Evolution,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
Highly
pathogenic
avian
influenza
(HPAI)
viruses
are
increasingly
spreading
between
birds
and
mammals
globally,
with
sporadic
transmission
to
humans.
With
recent
emergence
in
Antarctica,
traditional
animal
capture
testing
approaches
have
proven
challenging
logistically
impractical.
Without
reference
laboratories
the
region,
responses
slow
few
samples
will
ever
be
collected
or
tested
from
local
outbreaks
due
lack
of
infrastructure.
We
call
for
development
innovative
data
collection
strategies
that
can
deployed
a
diverse
range
sample
types
rapid,
field-forward
characterization.
Policy
shifts
enhanced
biosecurity
protocols
required
protect
Antarctic
biodiversity,
we
advocate
global
coordination
strengthened
collaborations
national
programs,
tour
operators,
scientists
establish
'smart
surveillance'
network.
Язык: Английский