Опубликована: Дек. 23, 2024
Язык: Английский
Virus Research, Год журнала: 2024, Номер 348, С. 199438 - 199438
Опубликована: Июль 19, 2024
Previous studies have emphasized the necessity of surveillance and control measures for hepatitis E virus (HEV) infection in wild boars, an important reservoir HEV. To assess current situation HEV boars Japan, this study investigated prevalence genetic diversity among captured 16 prefectures Japan during 2018-2023. Serum samples from 968 were examined anti-HEV IgG antibodies RNA. The varied geographically 0 % to 35.0 %. RNA was detected 3.6 with varying by prefecture 22.2 Genotype 3 most prevalent genotype (91.9 %), followed 4 (5.4 one strain closely related 6. decreased 2018/2019 2022/2023 significant declines levels (14.5 vs. 6.2 %, P < 0.0001) (7.6 1.5 0.0001). Regional analysis showed trends, no RNA-positive found several regions recent years. A plausible factor contributing decline is application countermeasures, including installing fences prevent intrusion into pig farms, implemented response emergence classical swine fever (CSFV) domestic pigs, incidents reported annually since 2018. Further investigation warranted explore association between countermeasures CSFV decrease boars.
Язык: Английский
Процитировано
3
Viruses, Год журнала: 2024, Номер 16(9), С. 1400 - 1400
Опубликована: Авг. 31, 2024
The zoonotic transmission of hepatitis E virus (HEV) genotypes 3 (HEV-3) and 4 (HEV-4), rabbit HEV (HEV-3ra) has been documented. Vaccination against infection depends on the capsid (open reading frame 2, ORF2) protein, which is highly immunogenic elicits effective virus-neutralizing antibodies. Escherichia coli (E. coli) utilized as an system for producing HEV-like particles (VLPs). However, research production ORF2 proteins from these in E. to form VLPs modest. In this study, we constructed 21 recombinant plasmids expressing various N-terminally C-terminally truncated HEV-3, HEV-3ra, HEV-4 coli. We successfully obtained nine two ten proteins, were primarily localized inclusion bodies. These solubilized M urea, filtered, subjected gel filtration. Results revealed that six one could assemble into VLPs. purified displayed molecular weights ranging 27.1 63.4 kDa demonstrated high purity (74.7–95.3%), assessed by bioanalyzer, with yields 13.9–89.6 mg per 100 mL TB medium. Immunoelectron microscopy confirmed origin ORF2. Antigenicity testing indicated possess characteristic antigenicity. Evaluation immunogenicity Balb/cAJcl mice robust anti-HEV IgG responses, highlighting potential immunogens. findings suggest generated different serve valuable tools vaccine development.
Язык: Английский
Процитировано
1
Pathogens, Год журнала: 2024, Номер 13(10), С. 840 - 840
Опубликована: Сен. 27, 2024
The hepatitis E virus (HEV) has become increasingly important in recent years terms of risk for public health, as the main causative agent acute viral hepatitis. It is a foodborne disease transmitted to humans through consumption contaminated water or food. Human-to-human transmission sporadic and linked transfusions transplants. reservoirs are domestic pigs wild boars, although, compared pigs, boars represent lesser source since their population smaller derived products more limited. These peculiarities often make role boar reservoir spread underestimated. As health problem that involves several animal species humans, management requires an interdisciplinary approach, concept “One Health” must be addressed. In this direction, present review intends analyze E, with particular focus on boar. For purpose, literature data have been collected from different scientific search engines: PubMed, MEDLINE, Google scholar, keywords such “HEV epidemiology”, “Extrahepatic manifestations Hepatitis E”, infection control measures”, among others, used. first part, manuscript provides general information disease, epidemiology, methods, clinical implications health. second it addresses detail related epidemiology. document will useful all those who intend infectious “One-Health” perspective.
Язык: Английский
Процитировано
1
Virus Research, Год журнала: 2024, Номер 347, С. 199427 - 199427
Опубликована: Июнь 27, 2024
The hepatitis E virus (HEV) is infecting over 20 million people annually with a high morbidity especially in pregnant women and immune-suppressed individuals. While HEV genotype 1 (HEV-1) infects only humans, 3 (HEV-3) zoonotic commonly transmitted from infected animals to humans. Whereas few reverse genetics systems enabling targeted genome manipulations exist for HEV-3, those HEV-1 are still very limited, mainly because of inefficient cell culture replication. Here, the generation strain Sar55 HEV-3 47832mc by transfecting vitro-transcribed capped genomes into different lines was attempted. Culture supernatants colon-derived colorectal adenocarcinoma line Caco-2 contained capable cells. Density gradient centrifugation analyses confirmed that particles were quasi-enveloped analogy non-virion-associated capsid protein secreted Following transfection or infection cells, consistently reached higher titers than supernatants, but generated cells unable efficiently infect hepatoma PLC/PRF/5 HuH7-Lunet BLR. Taken together, our results indicate able exert complete replication cycle An efficient system this will be useful studying species tropism, further research required determine significance
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2024, Номер 16(9), С. 1378 - 1378
Опубликована: Авг. 29, 2024
Hepatitis E virus (HEV) poses a significant global health threat, with an estimated 20 million infections occurring annually. Despite being self-limiting illness, in most cases, HEV infection can lead to severe outcomes, particularly pregnant women and individuals pre-existing liver disease. In the absence of specific antiviral treatments, exploration RNAi interference (RNAi) as targeted strategy provides valuable insights for urgently needed therapeutic interventions against E. We designed small interfering RNAs (siRNAs) HEV, which target helicase domain open reading frame 3 (ORF3). These regions will reduce risk viral escape through mutations, they belong conserved genome. The siRNAs targeting ORF3 efficiently inhibited replication A549 cells after infection. Importantly, siRNA was also highly effective at inhibiting persistently infected cell line, suitable model chronic patients. Furthermore, we showed that 5' triphosphate modification on sense strand activates RIG-I receptor, cytoplasmic pattern recognition receptor recognizes RNA. Upon activation, triggers signaling cascade, effectively suppressing replication. This dual-action strategy, combining activation adaptive immune response inherent pathway, inhibits successfully may development new therapies.
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2024, Номер 16(10), С. 1605 - 1605
Опубликована: Окт. 12, 2024
Since Mongolian gerbils are broadly susceptible to hepatitis E virus (HEV), including genotypes 1, 4, 5, and 8 (HEV-1, HEV-5, HEV-8) rat HEV, they a useful small animal model for HEV. However, we have observed that the subtypes HEV-3k HEV-3ra in genotype 3 HEV (HEV-3) were not infected efficiently gerbils. A small-animal HEV-3 is also needed since responsible major zoonotic infections. To investigate whether can be used as models other of HEV-3, injected with five (HEV-3b, -3e, -3f, -3k, -3ra) compared infectivity subtypes. We detected viral RNA gerbils' feces. High titers anti-HEV IgG antibodies serum induced all HEV-3b/ch-, HEV-3f-, HEV-3e-injected Especially, animals released high levels viruses into their feces an extended period. The replication was limited HEV-3b/wb-injected HEV-3k-injected groups. Although HEV-3ra-injected gerbils, copy numbers fecal specimens low; no sera. These results indicate although HEV-3's depends on subtype strain, potential HEV-3. further comparison HEV-3e different strains (HEV-4i HEV-5) genera (rat HEV) revealed ALT elevations among strains, liver damage occurred HEV-4i- HEV-5-infected but HEV-3e- or HEV-infected demonstrating variable pathogenicity across HEVs from might candidate examine HEV's pathogenicity.
Язык: Английский
Процитировано
0
Virology Journal, Год журнала: 2024, Номер 21(1)
Опубликована: Ноя. 26, 2024
Hepatitis E is recognized as a significant zoonotic disease burden in China, with the hepatitis virus (HEV) identified etiological agent responsible for this disease. HEV exhibits no specific host tropism, which facilitates its transmission among various mammalian species, including humans, pigs, cattle, goats, and others. Currently, availability of effective therapeutic agents vaccines infection limited. Therefore, comprehensive understanding epidemiological characteristics HEV, existing detection methods, crucial prevention control. In review, we provide an overview current knowledge on mainly focusing strategies, molecular characteristics, prevalence pathogen human population other susceptible species. This review will be useful to enhance public awareness accelerate control efforts future.
Язык: Английский
Процитировано
0
Veterinary Medicine inter-departmental subject scientific collection, Год журнала: 2024, Номер 110, С. 65 - 70
Опубликована: Окт. 23, 2024
Hepatitis E virus (HEV) infects humans and several mammals it has 8 genotypes (HEV1 HEV8). HEV1 4 is the causative agent of in humans. HEV5 6 was detected only wild boar Japan, HEV7 camels. HEV3 characterized by zoonotic potential. Swines boars are main natural reservoirs for this virus. Besides, HEV 3 deers, dolphins, rabbits, cattle, goats that additional risk interspecies transmission from domestic animals to Availability mismatched nucleotides complexes primer/probe with viral targets applied estimation primer sets. Based on determined conserved fragments two sets LNA containing linear probe without single stranded amplicon real time reverse transcriptase PCR detection swine isolates were designed. Primer may be used standard
Язык: Английский
Процитировано
0
Опубликована: Дек. 23, 2024
Язык: Английский
Процитировано
0