Molecular Detection of HPV E7 Gene Using TaqMan Probe-Based RT-PCR: A Case-Control Study in Hilla City, Iraq

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Год журнала: 2025, Номер 8(1), С. 106 - 111

Опубликована: Фев. 18, 2025

Background: Human papillomavirus (HPV) is a key driver of cervical cancer, with high-risk genotypes, mainly HPV 16 and 18, responsible for approximately 70% cases. Persistent infections immunological evasion by increase the risk malignant transformation. Objective: To evaluate effect TaqMan probes in real-time PCR pivotal role DNA detection, targeting highly conserved regions like E7 gene. Methods: A case-control study was conducted on 71 women Hilla City, Iraq, to detect DNA. The used swabs blood samples, Statistical analysis, including chi-square tests, evaluated associations between demographic, clinical, behavioral variables. Results: Out 22 tested positive HPV, primarily using (95.5%). Amplification gene showed high efficiency (100.9%) specificity. Significant were found infection factors such as smoking, rural residence, family history cancers. No co-infections observed. Conclusions: This underscores effectiveness probe-based RT-PCR detection highlights critical demographic factors. findings advocate expanding vaccination diagnostic access, particularly underserved regions.

Язык: Английский

The role of 5-fluorouracil in Wnt/β-catenin signalling in human papillomavirus-positive cervical cancer cells.

Investigación Clínica, Год журнала: 2025, Номер 66(1), С. 78 - 88

Опубликована: Март 7, 2025

Human papillomavirus (HPV) infection is a major risk factor for cer-vical cancer, especially persistent with high-risk HPV. 5-fluorouracil (5-FU) widely used antimetabolite chemotherapeutic agent that inhibits the prolifera-tion of tumour cells by interfering ribonucleic acid and deoxyribonucleic synthesis; however, its mechanism action has not been fully elucidated. This study aimed to investigate role Wnt/β-catenin signalling in patients HPV cervical cancer treated 5-FU. Patients HPV-positive surgery were taken as research participants, le-sion tissues collected during surgery. isolated cultured vitro enzyme combined digestion method, obtained divided into control group, paclitaxel group 5-FU group. A 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was measure proliferation under different treatment conditions. Western blotting evaluate protein expression level pathway cells, flow cytometry analyse T lymphocytes patients’ blood. The results MTT showed rate significantly higher than at all detection time points (p<0.05). levels lower those groups lymphocyte comparison ratios CD3+ CD4+ CD4+/CD8+ affected before can reduce increase cells.

Язык: Английский

Triple Viral Infections in Advanced Breast Cancer: Insights from a Three-Case Report and Literature Review

Diagnostics, Год журнала: 2024, Номер 15(1), С. 51 - 51

Опубликована: Дек. 28, 2024

Background and clinical significance: Viral infections are typically considered contributing or secondary factors in the development of breast cancer. Case presentation: This case report presents three instances advanced cancer associated with triple viral infections. 1 involves a 78-year-old woman diagnosed grade 2 invasive ductal carcinoma positive for HPV-18, CMV, BLV. describes 39-year-old carcinoma, HPV-16, 3 is 52-year-old BLV, MMTV. These cases suggest possible correlation between co-infections aggressiveness, posing new questions about role prognosis. Conclusions: The findings contribute to growing evidence that may influence progression therapeutic response cancer, warranting further investigation into targeted preventive measures including vaccinations.

Язык: Английский