CRISPR-Based Multiplex Detection of Human Papillomaviruses for One-Pot Point-of-Care Diagnostics

ACS Synthetic Biology, Год журнала: 2024, Номер 13(3), С. 837 - 850

Опубликована: Фев. 13, 2024

The World Health Organization's global initiative toward eliminating high-risk Human Papillomavirus (hrHPV)-related cancers recommends DNA testing over visual inspection in all settings for primary cancer screening and HPV eradication by 2100. However, multiple hrHPV types cause different of cancers, there is a pressing need an easy-to-use, multiplex point-of-care diagnostic platform detecting types. Recently, CRISPR-Cas systems have been repurposed detection. Here, we established multiplexed assay (CRISPRD) to detect cervical cancer-causing hrHPVs one reaction (one-pot assay). We harnessed the compatibility thermostable AapCas12b, TccCas13a, HheCas13a nucleases with isothermal amplification successfully detected HPV16 HPV18, along internal control single-pot limit detection 10 copies 100% specificity. This offers rapid practical solution hrHPVs, which may facilitate large-scale screening. Furthermore, CRISPRD programmability enables it be adapted any two nucleic acid biomarkers as well control.

Язык: Английский

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Knowledge and attitudes about human papillomavirus infection and prevention methods among medical science students in Novi Sad, Serbia: a cross-sectional study

BMC Public Health, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 27, 2025

It is estimated that more than 80% of people are infected with human papilloma virus (HPV) at some point in their lives. Knowledge about HPV infection and methods its prevention important for society as a whole. Medical students future healthcare workers must be thoroughly informed order to properly participate the promotion prevention. The aim was examine knowledge attitudes relation previous education methods. A cross-sectional study conducted among undergraduate Faculty Medicine Novi Sad (MFNS). covered all programs period from 1 30 November 2023, using specially created survey questionnaire purposes this research. 1760 surveys were collected, representing 70.4% total number MFNS students. In past year, only 26% respondents attended kind infection/vaccine. Statistical significance observed difference between responses who comparison those did not attend year (75.5% versus 63.3%, p < 0.001). Students stated they plan get vaccinated following answered questions correctly higher percentage (87.5% 80.1%, = 0.01). Sad, workers, demonstrated certain right attitudes. had correct answers, future.

Язык: Английский

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A decade data of HPV genotypes in metropolitan regions of Indonesia: paving the way for a national cervical cancer elimination strategy

Journal of Gynecologic Oncology, Год журнала: 2025, Номер 36

Опубликована: Янв. 1, 2025

Human papillomavirus (HPV) infection is a global public health concern and associated with cervical cancer. HPV genotype mapping has an essential role in prevention control strategy developing more suitable vaccine for Indonesia. This was descriptive retrospective cross-sectional study from 2012 until 2022 at Kalgen Laboratory, Jakarta all over the metropolitan regions. The total 76,413 samples were collected consecutive sampling, which 694 excluded, thus final used 75,719. DNA test performed using polymerase chain reaction (PCR): SPF10-DEIA-LiPA25 methods. genotyping procedures included extraction, PCR XpressMatrix kit, hybridization. From 75,719 samples, 93.4% negative intraepithelial lesion or malignancy (NILM). Among 6.6% of abnormal cytology groups, 53.8% atypical squamous cells undetermined significance (ASCUS), 32.9% low grade (LSIL), 13.3% high (HSIL). most common risk genotypes among HSIL 16, 18, 52, 58, 33, 51, 53. Single compared to multiple infections. showed that HR-HPV types 52 frequent type NILM, ASCUS, LSIL. Thus, it could serve as potential future reference create nonavalent Indonesian population based on its different epidemiology.

Язык: Английский

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Basic Knowledge About Cervical Cancer and its Screening Tests Among Secondary School Teachers in Baghdad City

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Год журнала: 2025, Номер 8(1), С. 215 - 220

Опубликована: Март 17, 2025

Background: Cervical cancer is a well-defined and preventable major health problem. The main cause behind it persistent human papillomavirus (HPV) infection. There are more than 200 HPV subtypes, but only minority of them oncogenic. mode transmission this infection by sexual intercourse. It can be prevented regular checking for premalignant states taking cervical smears (Pap smears) vaccinating young girls. In Iraq, there no official policies the preventive measures, personal awareness way to make women seek screening tests or vaccines. Objectives: To explore general information secondary school teachers in Baghdad city/Iraq who college graduates about cancer. Methods: A cross-sectional study was done, including 232 female were interviewed asked fill out written questionnaire. Results: Only 5.6% respondents mentioned viral as cancer, 14.2% answered sexually transmitted disease. 14.7% included recognize smear test. one teacher (0.4%) knows vaccine prevent Teaching material mostly has significant association with teacher’s information. source social media (76.7%). Conclusions: poor knowledge among educated class, teachers, affect rate occurrence disease future. Social used disseminate regarding Iraqi women.

Язык: Английский

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Human Papillomaviruses: An Ancient Enemy

Опубликована: Янв. 1, 2025

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Human Papillomavirus Carcinogenicity and the Need of New Perspectives: Thoughts from a Retrospective Analysis on Human Papillomavirus Outcomes Conducted at the Hospital University of Bari, Apulia, Italy, between 2011 and 2022

Diagnostics, Год журнала: 2024, Номер 14(9), С. 968 - 968

Опубликована: Май 6, 2024

Background: The current manuscript’s aim was to determine the human papillomavirus (HPV) genotype-specific prevalence and distribution among individuals, males, females, of different ages in region Apulia, Italy, highlighting possible variables involved carcinogenicity mechanism. In addition, we proposed two hypothetical models HPV’s molecular dynamics, intending clarify impact prevention therapeutic strategies, explicitly modeled by recent survey data. Methods: We presented clinical data from 9647 participants tested for either high-risk (HR) or low-risk (LR) HPV at affiliated Bari Policlinic University Hospital 2011 2022. DNA detection performed using nested-polymerase chain reaction (PCR) multiplex real-time PCR assay. Statistical analysis showed significant associations all genders both HR- LR-HPV types. A major number pairwise were detected higher-risk types females lower-risk males. Results: overall 50.5% (n-4.869) vs. 49.5% (n-4.778) study population, which 74.4% (n-3621) found be (HR-HPV) genotypes 57.7% (n-2.807) (LR-HPV) genotypes, males 58% 49%; three most prevalent HR-HPV 53 (n707-15%), 16 (n704-14%), 31 (n589-12%), LR-HPV, they 42 (19%), 6 (16%), 54 (13%); 56% patients screened ≤ 30 years old, 53% between 40 46% 41–50 51–60 finally, 44% subjects >60 old. Conclusions: Our provided comprehensive epidemiological on genotype participants, could serve as a reference practice, it implied necessity more effective screening methods carcinogenesis covering use specific investigations. Although this is predominantly descriptive study, obtained offer not only fairly unique trend compared other studies realities latitudes but also lead us focus infection within groups young people adults hypothesize involvement dysbiosis, stem cells, retrotransposition

Язык: Английский

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The Assessment of TikTok as a Source of Quality Health Information on Human Papillomavirus: A Content Analysis

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Дек. 9, 2024

Background Various studies have evaluated the quality of health-related information on TikTok (ByteDance Ltd., Beijing, China), including topics such as COVID-19, diabetes, varicoceles, bladder cancer, colorectal and others. However, there is a paucity data that examined source health human papillomavirus (HPV). This study, therefore, HPV TikTok. Methods The terms "HPV" "human papillomavirus" were searched single day in August 2024, 200 videos retrieved. Relevant user metrics collected for each video, number likes, shares, followers, video length, uploader type. Two independent raters assessed regarding completeness six types content (the definition HPV, symptoms, risk factors, evaluation, management, outcomes). Then, two independently using DISCERN instrument. Results Sixty-nine met inclusion criteria; 11 created by general users, 44 healthcare professionals, 14 organizations. Videos uploaded users professionals longer duration (p < 0.001) more likes = 0.048) than those More 60% contained little or no assessed. Although reliability treatment choices higher among overall instrument was "very poor" (24.2 (±6.92)). Conclusions very poor not acceptable, thus failing to satisfy public needs. Healthcare must enhance their social media presence, produce reliable substantive material, collaborate with platforms high-engagement accounts facilitate users' access high-quality data. recognize material may lack medical accuracy should consistently consult providers guidance.

Язык: Английский

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Human Papillomavirus Vaccination in Pediatric, Adolescent, and Young Adult Cancer Survivors—Opportunity to Address Gaps in Cancer Prevention and Survivorship

Vaccines, Год журнала: 2024, Номер 12(2), С. 114 - 114

Опубликована: Янв. 24, 2024

The risks of secondary cancers associated with human papillomavirus (HPV) infection are as much three times higher for survivors pediatric, adolescent, and young adult cancer (PYAC) compared to the general population. Despite this, HPV vaccination rates among PYAC remain low. Whereas pediatric oncology providers endorse survivors, many lack resources or opportunities intervene. responsibility vaccination, therefore, falls primary care practices. This article provides an overview challenges that distinct discusses potential strategies increase vaccine coverage in this

Язык: Английский

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STINGing Defenses: Unmasking the Mechanisms of DNA Oncovirus-Mediated Immune Escape

Viruses, Год журнала: 2024, Номер 16(4), С. 574 - 574

Опубликована: Апрель 9, 2024

DNA oncoviruses represent an intriguing subject due to their involvement in oncogenesis. These viruses have evolved mechanisms manipulate the host immune response, facilitating persistence and actively contributing carcinogenic processes. This paper describes complex interactions between innate system, with a particular emphasis on cGAS-STING pathway. Exploring these highlights that strategically target subvert this pathway, exploiting its vulnerabilities for own survival proliferation within host. Understanding lays foundation identifying potential therapeutic interventions. Herein, we sought contribute ongoing efforts advancing our understanding of system oncoviral pathogenesis.

Язык: Английский

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Efficacy of LCMV-based cancer immunotherapies is unleashed by intratumoral injections of polyI:C

Journal for ImmunoTherapy of Cancer, Год журнала: 2024, Номер 12(4), С. e008287 - e008287

Опубликована: Апрель 1, 2024

Background Lymphocytic choriomeningitis virus (LCMV) belongs to the Arenavirus family known for inducing strong cytotoxic T-cell responses in both mice and humans. LCMV has been engineered development of cancer immunotherapies, currently undergoing evaluation phase I/II clinical trials. Initial findings have demonstrated safety an exceptional ability activate expand tumor-specific T lymphocytes. Combination strategies maximize antitumor effectiveness LCMV-based immunotherapies are being explored. Methods We assessed therapeutic effects intratumoral administration polyinosinic:polycytidylic acid (poly(I:C)) systemic vaccination using LCMV-vector expressing non-oncogenic versions E6 E7 antigens human papillomavirus 16 (artLCMV-E7E6) a bilateral model engrafting TC-1/A9 cells. This cell line, derived from parental TC-1, exhibits low MHC class I expression is highly immune-resistant. The mechanisms underlying combination’s efficacy were investigated through bulk RNA-seq, flow cytometry analyses tumor microenvironment, selective depletions antibodies clodronate liposomes, Batf3 deficient mice, vivo bioluminescence experiments. Finally, we combination artLCMV-E7E6 with BO-112, GMP-grade poly(I:C) formulated polyethyleneimine, under Results Intratumoral injection enhanced injected non-injected lesions. combined treatment resulted significant delay growth often complete eradication several lesions, leading significantly improved survival compared monotherapies. While did not impact vector biodistribution or transgene expression, it modified leucocyte infiltrates within microenvironment amplified proinflammatory cytokines/chemokines such as CCL3, CCL5, CXCL10, TNF, IFNα, IL12p70. Upregulation on cells reconfiguration gene programs related vasculature, migration, activation profile tumor-infiltrating CD8 + lymphocytes observed. Indeed, effect relied functions macrophages. synergistic was further confirmed when BO-112 included. Conclusion sensitizes tumors artLCMV-E7E6, resulting potent synergy.

Язык: Английский

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