A respiratory mucosal vaccine based on chitosan/aluminum adjuvant induces both mucosal and systemic immune responses
International Journal of Pharmaceutics,
Год журнала:
2025,
Номер
670, С. 125168 - 125168
Опубликована: Янв. 5, 2025
Язык: Английский
The benefits of Lactiplantibacillus plantarum: from immunomodulator to vaccine vector
Immunology Letters,
Год журнала:
2025,
Номер
272, С. 106971 - 106971
Опубликована: Янв. 5, 2025
Probiotics
have
been
increasingly
recognized
for
positively
influencing
many
aspects
of
human
health.
Lactiplantibacillus
plantarum
(L.
plantarum),
a
non-pathogenic
bacterium,
previously
known
as
Lactobacillus
plantarum,
is
one
the
lactic
acid
bacteria
commonly
used
in
fermentation.
The
probiotic
properties
L.
highlighted
its
health
benefits
to
humans
when
consumed
adequate
amounts.
strains
primarily
enter
body
orally
and
alter
intestinal
microflora
modulate
immune
responses
their
host;
thereby
benefiting
Furthermore,
use
vaccine
vectors
delivering
mucosal
antigens
has
shown
be
promising
strategy.
These
aspects,
from
Immunomodulation
delivery
by
preclinical
settings,
are
this
review.
Along
these
lines,
construction
recombinant
strain
expressing
B
cell
multi-peptide,
future
immunity
confer
anti-tumor
effect
targeting
Her-2/neu-overexpressing
cancers
local
distal
sites,
also
presented
discussed.
Язык: Английский
SARS-CoV-2 Evolution: Implications for Diagnosis, Treatment, Vaccine Effectiveness and Development
Vaccines,
Год журнала:
2024,
Номер
13(1), С. 17 - 17
Опубликована: Дек. 28, 2024
The
COVID-19
pandemic,
driven
by
the
rapid
evolution
of
SARS-CoV-2
virus,
presents
ongoing
challenges
to
global
public
health.
is
characterized
rapidly
evolving
mutations,
especially
in
(but
not
limited
to)
spike
protein,
complicating
predictions
about
its
evolutionary
trajectory.
These
mutations
have
significantly
affected
transmissibility,
immune
evasion,
and
vaccine
efficacy,
leading
multiple
pandemic
waves
with
over
half
a
billion
cases
seven
million
deaths
globally.
Despite
several
strategies,
from
development
administration
design
availability
antivirals,
including
monoclonal
antibodies,
already
having
been
employed,
persistent
circulation
virus
emergence
new
variants
continue
result
high
case
numbers
fatalities.
In
past
four
years,
immense
research
efforts
contributed
much
our
understanding
viral
pathogenesis
mechanism,
syndrome,
host-microbe
interactions,
effective
vaccines,
diagnostic
tools,
treatments.
focus
this
review
provide
comprehensive
analysis
functional
impact
on
diagnosis,
treatments,
effectiveness.
We
further
discuss
safety
pregnancy
implications
hybrid
immunity
long-term
protection
against
infection,
as
well
latest
developments
pan-coronavirus
nasal
formulations,
emphasizing
need
for
continued
surveillance,
research,
adaptive
health
strategies
response
race.
Язык: Английский
Dissecting Immunological Mechanisms Underlying Influenza Viral Nucleoprotein-induced Mucosal Immunity Against Diverse Viral Strains
Emerging Microbes & Infections,
Год журнала:
2024,
Номер
13(1)
Опубликована: Ноя. 7, 2024
The
nucleoprotein
(NP)
of
type
A
influenza
virus
(IAV)
is
highly
conserved
across
all
strains,
making
it
an
attractive
candidate
antigen
for
universal
vaccines.
While
various
studies
have
explored
NP-induced
mucosal
immunity,
here
we
interrogated
the
mechanistic
differences
between
intramuscular
(IM)
and
intranasal
(IN)
delivery
a
recombinant
adenovirus
carrying
NP
fused
with
bifunctional
CD40
ligand.
Despite
being
less
effective
than
IM
in
inducing
systemic
cellular
immune
responses
antibody-dependent
cytotoxicity
(ADCC),
IN
immunization
elicited
superior
antigen-specific
recall
humoral
response
nasal
associated
lymphoid
tissue
(NALT)
upper
respiratory
tract,
initial
site
recognition
elimination
inhaled
pathogens.
vaccination
also
induced
significantly
stronger
pulmonary
T
cell
lower
tract
vaccination,
particular
CD8
cells.
Moreover,
blocking
lymphocyte
circulation
abrogated
but
not
protection,
illustrating
critical
role
local
memory
upon
viral
infection.
Notably,
CD40-targeted
only
improved
magnitude
breadth
including
against
lethal
challenge
newly
isolated
pathogenic
avian
H5N1
strain.
These
findings
are
informative
design
vaccines,
where
predominant
mode
protection
independent
neutralizing
antibodies.
Язык: Английский
B and T Cell Bi-Cistronic Multiepitopic Vaccine Induces Broad Immunogenicity and Provides Protection Against SARS-CoV-2
Vaccines,
Год журнала:
2024,
Номер
12(11), С. 1213 - 1213
Опубликована: Окт. 25, 2024
Background:
The
COVID-19
pandemic,
caused
by
SARS-CoV-2,
has
highlighted
the
need
for
vaccines
targeting
both
neutralizing
antibodies
(NAbs)
and
long-lasting
cross-reactive
T
cells
covering
multiple
viral
proteins
to
provide
broad
durable
protection
against
emerging
variants.
Methods:
To
address
this,
here
we
developed
two
vaccine
candidates,
namely
(i)
DNA-CoV2-TMEP,
expressing
multiepitopic
CoV2-TMEP
protein
containing
immunodominant
conserved
cell
regions
from
SARS-CoV-2
structural
proteins,
(ii)
MVA-CoV2-B2AT,
encoding
a
bi-cistronic
construct
that
combines
B
overlapping
proteins.
Results:
Both
candidates
were
assessed
in
vitro
vivo
demonstrating
their
ability
induce
robust
immune
responses.
In
C57BL/6
mice,
DNA-CoV2-TMEP
enhanced
recruitment
of
innate
stimulated
SARS-CoV-2-specific
polyfunctional
MVA-CoV2-B2AT
elicited
NAbs
various
variants
concern
(VoCs)
reduced
replication
yields
Beta
variant
susceptible
K18-hACE2
mice.
combination
with
mutated
ISG15
form
as
an
adjuvant
further
increased
magnitude,
breadth
profile
response.
Conclusion:
These
findings
underscore
potential
these
when
expressed
DNA
or
MVA
vectors
its
variants,
supporting
development
next-generation
vaccines.
Язык: Английский