B and T Cell Bi-Cistronic Multiepitopic Vaccine Induces Broad Immunogenicity and Provides Protection Against SARS-CoV-2 DOI Creative Commons
Beatriz Perdiguero, Enrique Álvarez, Laura Marcos-Villar

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1213 - 1213

Published: Oct. 25, 2024

Background: The COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the need for vaccines targeting both neutralizing antibodies (NAbs) and long-lasting cross-reactive T cells covering multiple viral proteins to provide broad durable protection against emerging variants. Methods: To address this, here we developed two vaccine candidates, namely (i) DNA-CoV2-TMEP, expressing multiepitopic CoV2-TMEP protein containing immunodominant conserved cell regions from SARS-CoV-2 structural proteins, (ii) MVA-CoV2-B2AT, encoding a bi-cistronic construct that combines B overlapping proteins. Results: Both candidates were assessed in vitro vivo demonstrating their ability induce robust immune responses. In C57BL/6 mice, DNA-CoV2-TMEP enhanced recruitment of innate stimulated SARS-CoV-2-specific polyfunctional MVA-CoV2-B2AT elicited NAbs various variants concern (VoCs) reduced replication yields Beta variant susceptible K18-hACE2 mice. combination with mutated ISG15 form as an adjuvant further increased magnitude, breadth profile response. Conclusion: These findings underscore potential these when expressed DNA or MVA vectors its variants, supporting development next-generation vaccines.

Language: Английский

A respiratory mucosal vaccine based on chitosan/aluminum adjuvant induces both mucosal and systemic immune responses DOI

Ruitong Chen,

Meifeng Nie,

Yili Jiang

et al.

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 670, P. 125168 - 125168

Published: Jan. 5, 2025

Language: Английский

Citations

2
The benefits of Lactiplantibacillus plantarum: from immunomodulator to vaccine vector DOI Creative Commons
Joshua Tobias,

Stefan Heinl,

Kristina Dendinovic

et al.

Immunology Letters, Journal Year: 2025, Volume and Issue: 272, P. 106971 - 106971

Published: Jan. 5, 2025

Probiotics have been increasingly recognized for positively influencing many aspects of human health. Lactiplantibacillus plantarum (L. plantarum), a non-pathogenic bacterium, previously known as Lactobacillus plantarum, is one the lactic acid bacteria commonly used in fermentation. The probiotic properties L. highlighted its health benefits to humans when consumed adequate amounts. strains primarily enter body orally and alter intestinal microflora modulate immune responses their host; thereby benefiting Furthermore, use vaccine vectors delivering mucosal antigens has shown be promising strategy. These aspects, from Immunomodulation delivery by preclinical settings, are this review. Along these lines, construction recombinant strain expressing B cell multi-peptide, future immunity confer anti-tumor effect targeting Her-2/neu-overexpressing cancers local distal sites, also presented discussed.

Language: Английский

Citations

0
SARS-CoV-2 Evolution: Implications for Diagnosis, Treatment, Vaccine Effectiveness and Development DOI Creative Commons
Fabrizio Angius,

Silvia Puxeddu,

S Zaimi

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 13(1), P. 17 - 17

Published: Dec. 28, 2024

The COVID-19 pandemic, driven by the rapid evolution of SARS-CoV-2 virus, presents ongoing challenges to global public health. is characterized rapidly evolving mutations, especially in (but not limited to) spike protein, complicating predictions about its evolutionary trajectory. These mutations have significantly affected transmissibility, immune evasion, and vaccine efficacy, leading multiple pandemic waves with over half a billion cases seven million deaths globally. Despite several strategies, from development administration design availability antivirals, including monoclonal antibodies, already having been employed, persistent circulation virus emergence new variants continue result high case numbers fatalities. In past four years, immense research efforts contributed much our understanding viral pathogenesis mechanism, syndrome, host-microbe interactions, effective vaccines, diagnostic tools, treatments. focus this review provide comprehensive analysis functional impact on diagnosis, treatments, effectiveness. We further discuss safety pregnancy implications hybrid immunity long-term protection against infection, as well latest developments pan-coronavirus nasal formulations, emphasizing need for continued surveillance, research, adaptive health strategies response race.

Language: Английский

Citations

3
Dissecting Immunological Mechanisms Underlying Influenza Viral Nucleoprotein-induced Mucosal Immunity Against Diverse Viral Strains DOI Creative Commons

Wanyue Zhang,

Angela Sloan, Jérémie Prévost

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)

Published: Nov. 7, 2024

The nucleoprotein (NP) of type A influenza virus (IAV) is highly conserved across all strains, making it an attractive candidate antigen for universal vaccines. While various studies have explored NP-induced mucosal immunity, here we interrogated the mechanistic differences between intramuscular (IM) and intranasal (IN) delivery a recombinant adenovirus carrying NP fused with bifunctional CD40 ligand. Despite being less effective than IM in inducing systemic cellular immune responses antibody-dependent cytotoxicity (ADCC), IN immunization elicited superior antigen-specific recall humoral response nasal associated lymphoid tissue (NALT) upper respiratory tract, initial site recognition elimination inhaled pathogens. vaccination also induced significantly stronger pulmonary T cell lower tract vaccination, particular CD8 cells. Moreover, blocking lymphocyte circulation abrogated but not protection, illustrating critical role local memory upon viral infection. Notably, CD40-targeted only improved magnitude breadth including against lethal challenge newly isolated pathogenic avian H5N1 strain. These findings are informative design vaccines, where predominant mode protection independent neutralizing antibodies.

Language: Английский

Citations

1
B and T Cell Bi-Cistronic Multiepitopic Vaccine Induces Broad Immunogenicity and Provides Protection Against SARS-CoV-2 DOI Creative Commons
Beatriz Perdiguero, Enrique Álvarez, Laura Marcos-Villar

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(11), P. 1213 - 1213

Published: Oct. 25, 2024

Background: The COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the need for vaccines targeting both neutralizing antibodies (NAbs) and long-lasting cross-reactive T cells covering multiple viral proteins to provide broad durable protection against emerging variants. Methods: To address this, here we developed two vaccine candidates, namely (i) DNA-CoV2-TMEP, expressing multiepitopic CoV2-TMEP protein containing immunodominant conserved cell regions from SARS-CoV-2 structural proteins, (ii) MVA-CoV2-B2AT, encoding a bi-cistronic construct that combines B overlapping proteins. Results: Both candidates were assessed in vitro vivo demonstrating their ability induce robust immune responses. In C57BL/6 mice, DNA-CoV2-TMEP enhanced recruitment of innate stimulated SARS-CoV-2-specific polyfunctional MVA-CoV2-B2AT elicited NAbs various variants concern (VoCs) reduced replication yields Beta variant susceptible K18-hACE2 mice. combination with mutated ISG15 form as an adjuvant further increased magnitude, breadth profile response. Conclusion: These findings underscore potential these when expressed DNA or MVA vectors its variants, supporting development next-generation vaccines.

Language: Английский

Citations

0