Gualou-Xiebai herb pair ameliorate atherosclerosis in HFD-induced ApoE−/− mice and inhibit the ox-LDL-induced injury of HUVECs by regulating the Nrf2-mediated ferroptosis

Journal of Ethnopharmacology, Год журнала: 2024, Номер 326, С. 117892 - 117892

Опубликована: Фев. 11, 2024

Язык: Английский

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Regulation of inflammatory response by LINC00346 via miR-25-3p-mediated modulation of the PTEN/PI3K/AKT/NF-κB pathway

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 709, С. 149828 - 149828

Опубликована: Март 25, 2024

Язык: Английский

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MiR-148a-3p/SIRT7 Axis Relieves Inflammatory-Induced Endothelial Dysfunction

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5087 - 5087

Опубликована: Май 7, 2024

In endothelial cells, miR-148a-3p is involved in several pathological pathways, including chronic inflammatory conditions. However, the molecular mechanism of states is, to date, not fully elucidated. To this end, we investigated involvement mitochondrial dysfunction and cell death pathways human aortic cells (teloHAECs) treated with interleukin-6 (IL-6), a major driver vascular dysfunction. The results showed that during IL6-activated increased protein levels sirtuin 7 (SIRT7) (p < 0.01), stress 0.001), apoptosis decreased expression was observed 0.01). employment miR-148a mimic counteracted IL-6-induced cytokine release 0.01) apoptotic ameliorated mitochondria redox homeostasis respiration targeted relationship between SIRT7 predicted by bioinformatics database analysis validated via dual-luciferase reporter assay. Mechanistically, targets 3′ untranslated regions mRNA, downregulating its Herein, these vitro support role miR-148a-3p/SIRT7 axis counteracting damage inflammation, unveiling novel target for future strategies prevent

Язык: Английский

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LncRNA TMC3-AS1 silence alleviates lipopolysaccharide-induced acute kidney injury by suppressing Wnt5a-mediated autophagy and pyroptosis pathway

Molecular and Cellular Probes, Год журнала: 2025, Номер 79, С. 102006 - 102006

Опубликована: Янв. 21, 2025

Язык: Английский

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Enhancing Skin Injury Repair: Combined Application of PF-127 Hydrogel and hADSC-Exos Containing miR-148a-3p

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(4), С. 2235 - 2250

Опубликована: Март 6, 2024

The use of exosomes to relieve skin injuries has received considerable attention. PluronicF-127 hydrogel (PF-127 hydrogel) is a novel biomaterial that can be used carry biomolecules. This study sought investigate the impact originating from human mesenchymal stem cells (MSCs) developed adipose tissue (hADSC-Exos) combined with PF-127 on repair and explore underlying mechanism using in vitro vivo experiments. miR-148a-3p most expressed microRNA (miRNA) hADSC-Exos. We found had better efficacy than alone; moreover, knockdown lowered its efficacy. In vitro, we observed significant increase tumor-like ability HUVECs after exosome treatment, which was attenuated knockdown. Furthermore, effects hADSC-Exos were achieved through prevention PTEN triggering phosphatidylinositol 3-kinase (PI3K)/Akt signaling. conclusion, our results demonstrated promote angiogenesis wound healing by delivering have effect when hydrogel, may an alternative strategy healing.

Язык: Английский

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Epigenetic modifications as therapeutic targets in atherosclerosis: a focus on DNA methylation and non-coding RNAs

Frontiers in Cardiovascular Medicine, Год журнала: 2023, Номер 10

Опубликована: Май 25, 2023

Significant progress in the diagnosis and treatment of cardiovascular disease (CVD) has been made past decade, yet it remains a leading cause morbidity mortality globally, claiming an estimated 17.9 million deaths per year. Although encompassing any condition that affects circulatory system, including thrombotic blockage, stenosis, aneurysms, blood clots arteriosclerosis (general hardening arteries), most prevalent underlying hallmark CVD is atherosclerosis; plaque-associated arterial thickening. Further, distinct conditions have overlapping dysregulated molecular cellular characteristics which underlie their development progression, suggesting some common aetiology. The identification heritable genetic mutations associated with atherosclerotic vascular (AVD), particular resulting from Genome Wide Association Studies (GWAS) studies significantly improved ability to identify individuals at risk. However, increasingly recognised environmentally-acquired, epigenetic changes are key factors atherosclerosis development. Increasing evidence suggests these changes, notably DNA methylation misexpression non-coding, microRNAs (miRNAs) potentially both predictive causal AVD This, together reversible nature, makes them useful biomarkers for attractive therapeutic targets reverse progression. We consider here association aberrant miRNA expression aetiology progression atherosclerosis, potential novel cell-based strategies target therapeutically.

Язык: Английский

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Participation of Krüppel-like Factors in Atherogenesis

Metabolites, Год журнала: 2023, Номер 13(3), С. 448 - 448

Опубликована: Март 19, 2023

Atherosclerosis is an important problem in modern medicine, the keys to understanding many aspects of which are still not available clinicians. develops as a result complex chain events cells vascular wall and peripheral blood flow involved. Endothelial cells, line monolayer, play role biology. A growing body evidence strengthens multifaceted functions endothelial only organize barrier between tissues but also act regulators hemodynamics regulating function other wall. Krüppel-like factors (KLFs) perform several biological various The large family KLFs humans includes 18 members, among KLF2 KLF4 at crossroads cell mechanobiology immunometabolism, roles both normal atherosclerosis.

Язык: Английский

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MicroRNA-375 repression of Kruppel-like factor 5 improves angiogenesis in diabetic critical limb ischemia

Angiogenesis, Год журнала: 2022, Номер 26(1), С. 107 - 127

Опубликована: Сен. 8, 2022

Язык: Английский

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MiR-148a-3p attenuates apoptosis and inflammation by targeting CNTN4 in atherosclerosis

Annals of Translational Medicine, Год журнала: 2022, Номер 10(22), С. 1201 - 1201

Опубликована: Ноя. 1, 2022

Atherosclerosis (AS) seriously affects human health. The role of microRNAs (miRNAs) in the pathogenesis and progression AS has become a focus research. Our goal was to identify biological effect differentially expressed miRNAs (DE-miRNAs) AS.To analyze genes (DEGs), including mRNAs (DE-mRNAs) DE-miRNAs, by using Gene Expression Omnibus (GEO) database limma package. DEGs protein-protein interaction (PPI) network functional enrichment analysis were constructed search tool for retrieval interacting genes/proteins (STRING) database, Cytoscape software plugin "ClueGO2.5.6". We established coexpression dysregulated predict function miRWalk Pearson correlation coefficient (PCC) analysis. Cellular experiments used validate results bioinformatics.First, 69 common obtained from datasets GSE43292 GSE97210 package R. Next, DEG PPI constructed. Functional showed that 11 pathways significantly enriched, such as positive regulation monocyte chemotaxis. Seven DE-miRNAs GSE99685 dataset DE-mRNAs predicted through database. miRNA-mRNA suggested miR-148a-3p targeted contactin 4 (CNTN4). Quantitative real-time polymerase chain reaction (qRT-PCR) assay indicated downregulated CNTN4 upregulated THP-1 + phorbol 12-myristate 13-acetate (PMA) oxidized low-density lipoprotein (oxLDL) group compared with PMA group. qRT-PCR, flow cytometry, enzyme-linked immunosorbent (ELISA) found inhibited expression CNTN4, cell apoptosis, interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α) concentrations oxLDL-induced macrophages. In addition, dual-luciferase reporter demonstrated target gene miR-148a-3p.Overall, these findings apoptosis inflammation via targeting

Язык: Английский

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RNA sequencing-based identification of the regulatory mechanism of microRNAs, transcription factors, and corresponding target genes involved in vascular dementia

Frontiers in Neuroscience, Год журнала: 2022, Номер 16

Опубликована: Сен. 20, 2022

Vascular dementia (VaD) is the second most common form of with uncertain mechanisms and no effective treatments. microRNAs (miRNAs) transcription factors (TFs) are considered regulatory genes involved in many diseases. Therefore, this work investigated aberrantly expressed miRNAs, TFs, corresponding target genes, their co-regulatory networks cortex rats bilateral carotid artery occlusion (2VO) to uncover potential mechanism biomarkers VaD. Differentially (DEGs), miRNAs (DEMs), TFs (DETFs) were identified using RNA sequencing, interaction constructed Cytoscape. The results showed that 2VO had declined cognitive abilities neuronal loss than sham rats. DEGs, DEMs, DETFs discriminated between cortex, as shown by 13 805 mRNAs, 63 TFs. miRNA-TF-target gene network was constructed, showing 523 nodes 7237 edges. Five (miR-5132-5p, miR-764-3p, miR-223-3p, miR-145-5p, miR-122-5p), ten (Mxi1, Nfatc4, Rxrg, Zfp523, Foxj2, Nkx6-1, Klf4, Klf5, Csrnp1, Prdm6), seven (Serpine1, Nedd4l, Pxn, Col1a1, Plec, Trip12, Tpm1) chosen significant construct feed-forward loops (FFLs). Gene Ontology pathway enrichment analysis revealed these miRNA TF-associated mostly PI3K/Akt, neuroactive ligand-receptor interaction, calcium signaling, Wnt signaling pathways, along central locations around cell membrane. They exert functions such growth factor binding, integrin extracellular matrix structural constituent, representative biological processes like vasculature development, cell-substrate adhesion, cellular response stimulus, synaptic transmission. Furthermore, expression three (miR-145-5p, miR-122-5p, miR-5132-5p), six (Csrnp1, Klf5), five mRNAs significantly changed VaD, line outcome sequencing. In FFL, miR-145-5p directly bound Csrnp1 decreased its mRNA expression. These might help understanding underlying miRNA-TF-genes, providing therapeutic targets

Язык: Английский

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