Role of non-coding RNAs in the pathogenesis of viral myocarditis

Virulence, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Viral myocarditis (VMC) is a common inflammatory disease of the myocardium that characterized mainly by cell infiltration and cardiomyocyte necrosis. Coxsackievirus B3 (CVB3) cause VMC, although major progress has been made in treatment long-term prognosis still not ideal further research needed. Non-coding RNAs (ncRNAs) are RNA molecules without coding functions include microRNAs (miRNAs), long non-coding (lncRNAs) circular (circRNAs), which play extensive regulatory roles gene expression; however, their mechanisms action CVB3-induced VMC remain incompletely understood. Here, we review currently known various ncRNAs models, with focus on death, inflammation viral replication, aim providing reference for therapeutic or vaccine development VMC.

Язык: Английский

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circDENND4C serves as a sponge for miR-200b to drive non-small cell lung cancer advancement by regulating MMP-9 expression

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 17, 2025

Introduction Lung cancer has a higher incidence and mortality rate than other cancers, especially non-small cell lung (NSCLC), accounting for 85% of the cases. The role circDENND4C/miR-200b/matrix metalloproteinase-9 (MMP-9) regulatory axis in NSCLC remains largely unknown. Methods lines were used to examine expression circDENND4C, miR-200b, MMP-9 via qRT-PCR or Western blot. target relationship was examined by RNA fluorescence situ hybridization (RNA-FISH), immunofluorescence (IF), dual-luciferase reporter system, quantitative real-time polymerase chain reaction (qRT-PCR), Then, count kit-8 (CCK-8) experiment, flow cytometry, migration/invasion assays performed assess biological function transfecting with their overexpression knockout plasmids A549 cells. Finally, proteins related adhesion tight junction further tested blot IF. Results circDENND4C found be highly expressed lines, while miR-200b lowly lines. Moreover, could sponge MMP-9. Subsequently, it observed that knockdown upregulation repressed proliferation cycle progression, increased apoptosis, hindered migration invasion. also circDENND4C/miR-200b/MMP-9 might involved influence tumor metastasis. Conclusions Altogether, our study reveals novel loop which may modulate indicating potential biomarkers diagnosis treatment NSCLC.

Язык: Английский

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Evaluation of CircHIPK3 Biomarker Potential in Breast Cancer

Practical Laboratory Medicine, Год журнала: 2025, Номер unknown, С. e00470 - e00470

Опубликована: Март 1, 2025

Nowadays, the investigation of circular RNAs (circRNAs) in various cancers is great interest. In this research, we evaluated circHIPK3 biomarker potential breast cancer (BC). The studied samples were 100 and adjacent normal tissues, plasma from 95 patients, 42 patients with fibroadenomatosis, 93 healthy donors. Illumina high-throughput Hi Seq 2000 sequencing performed expression profiling on 4 pairs cancerous tissues. For confirmation, Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) was used to detect level circHIPK3. CircHIPK3 diagnostic efficacy by receiver operating characteristic curve (ROC). Based bioinformatics results had highest tissues (P = 0.00034). Real-time showed upregulation BC comparison controls < 0.0001). potential, area under (AUC) result 0.8087 (95 % CI: 0.7309 0.8866, P Also, our high specificity sensitivity when alongside CA-15-3 CEA. Pathologic criteria evaluation that correlates tumor size. significantly upregulated compared controls, demonstrating an AUC 0.8087. size, indicating its relevance pathologic assessment BC.

Язык: Английский

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hsa_circ_0008285: Circular RNA with potential as a biomarker in ovarian cancer

Current Problems in Cancer, Год журнала: 2025, Номер 56, С. 101208 - 101208

Опубликована: Апрель 26, 2025

Язык: Английский

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hsa_circ_0072309 Inhibits Oncogenesis in Hepatocellular Carcinoma by Epigenetic Activation of its Host Gene

Cell Biochemistry and Biophysics, Год журнала: 2024, Номер 82(4), С. 3251 - 3263

Опубликована: Сен. 16, 2024

Язык: Английский

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Crosstalk between circular RNAs and the STAT3 signaling pathway in human cancer

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Год журнала: 2024, Номер 1867(4), С. 195051 - 195051

Опубликована: Авг. 8, 2024

Язык: Английский

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hsa_circ_0088196 Predicts Adverse Pregnancy Outcomes in Preeclampsia Patients and Contributes to Endothelial Cell Injury Via Modulating the miR-145-5p/FLT1 Axis

Reproductive Sciences, Год журнала: 2024, Номер 31(12), С. 3825 - 3833

Опубликована: Авг. 12, 2024

Язык: Английский

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