Immunologic Research, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
Язык: Английский
Human Cell, Год журнала: 2025, Номер 38(2)
Опубликована: Фев. 14, 2025
Abstract Glioma is one of the most malignancy and prevalent tumor in human central nervous system, which associated with severe morbidity high mortality. Numerous studies have explained clear correlation between abnormal expression lncRNA progression Glioma. LncRNA small nucleolar RNA host gene 4 (SNHG4) been proved to play oncogenesis roles various tumors, however, underlying mechanism remains be explored deeply. In this study, by analysis public database, we found that SNHG4 was upregulated multiple cancer tissues, including glioma. Subsequently, functional were investigated, knockdown remarkedly inhibited cell proliferation, migration. While, overexpression enhanced these functions glioma cells vitro. Meanwhile, as vivo tool, zebrafish xenograft model used verify cells. Mechanically, identified or MYO1B could bind miR-367-3p luciferase reporter assays. Furthermore, rescue experiments showed inhibition partially effects Our study reveals promotes migration via regulating miR-367-3p/MYO1B axis.
Язык: Английский
Процитировано
1
Experimental Cell Research, Год журнала: 2025, Номер unknown, С. 114531 - 114531
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3629 - 3629
Опубликована: Апрель 11, 2025
Humanized tumor mice (HTM) allow for preclinical cancer treatment studies of breast (BC) under human-like conditions. This study utilized HTM the first time to investigate potential miRNA biomarker candidates response in sera and extracellular vesicles (EVs), following X-irradiation atezolizumab (anti-PD-L1) treatment. We identified changes human-specific miRNAs (miR-23b-3p miR-155-5p) after irradiation anti-PD-L1 HTMs with human epidermal growth factor receptor 2 positive (HER2+ BC) triple-negative (TNBC). The high degree conserved, circulating free men represents a challenge our assay; however, ≥2 nucleotide mismatches can be employed analysis, even conserved may clearly defined conditions HTM. A comparative analysis vesicle cargo free-circulating serum revealed several exosome-specific (miR-29b-3p, miR-34c-5p, miR-203a-3p, miR-378g, miR-382-5p) HTMs, which are known play roles BC. Our findings demonstrate that suitable model identify treatment-induced exosomal influence progression immunological defense, both locally at distant sites. presents proof-of-principle approach analyzing cell-free nucleotides exosomes human-like, vivo setting. Further refinements necessary enhance sensitivity specificity HTM-based approach.
Язык: Английский
Процитировано
0
Frontiers in Pharmacology, Год журнала: 2024, Номер 15
Опубликована: Июль 25, 2024
Long noncoding RNAs (lncRNAs) constitute a distinctive subset of RNA molecules with limited protein-coding potential, which exert crucial impacts on various biological activities. In the context cancer, dysregulated lncRNAs function as essential regulators that affect tumor initiation and malignant progression. These serve competitive endogenous (ceRNAs) through sponging microRNAs regulating expression targeted genes. Moreover, they also directly bind to RNA-binding proteins, can be integrated into complex mechanistic network. E2F1, an extensively studied transcription factor, mediates multiple behaviors by cell cycle progression, metastasis, therapeutic response. Emerging evidence suggests play pivotal role in E2F1 pathway. This review aims elucidate intricate gene regulatory programs between cancer We elaborate distinct networks involved emphasizing potential lncRNAs/E2F1 axes promising targets for therapy. Additionally, we provide novel perspectives current evidence, limitations, future directions targeting human cancers. Fully deciphering network lncRNA/E2F1-mediated mechanisms could facilitate translation findings clinical course, such efforts ultimately significantly improve prognosis patients.
Язык: Английский
Процитировано
3
Oncology Letters, Год журнала: 2024, Номер 28(4)
Опубликована: Авг. 8, 2024
Colorectal cancer (CRC) ranks as the 3rd most common globally and is 2nd leading cause of cancer‑related death. Owing to lack specific early symptoms limitations existing diagnostic methods, patients with CRC are diagnosed at advanced stages. To overcome these challenges, researchers have increasingly focused on molecular biomarkers, particular interest in long non‑coding RNAs (lncRNAs). These non‑protein‑coding RNAs, which exceed 200 nucleotides length, play critical roles development progression CRC. The stability detectability lncRNAs circulatory system make them promising candidate biomarkers. analysis circulating peripheral blood represents a potential option for minimally invasive tests based liquid biopsy samples. present review aimed evaluate efficacy altered expression levels markers Additionally, clinical significance prognostic this disease were summarized.
Язык: Английский
Процитировано
3
Frontiers in Oncology, Год журнала: 2024, Номер 14
Опубликована: Июль 4, 2024
CRC poses a significant challenge in the global health domain, with high number of deaths attributed to this disease annually. If is detected only its advanced stages, difficulty treatment increases significantly. Therefore, biomarkers for early detection play crucial role improving patient outcomes and increasing survival rates. The development reliable biomarker particularly important timely diagnosis treatment. However, current methods detection, such as endoscopic examination, blood, stool tests, have certain limitations often detect cases late stages. To overcome these constraints, researchers turned their attention molecular biomarkers, which are considered promising approach detection. Non-invasive using mRNA, circulating cell-free DNA, microRNA, LncRNA, proteins can provide more diagnostic information. These be found tissue, stool, volatile organic compounds. Identifying sensitivity specificity safe, economic, easily measurable remains researchers.
Язык: Английский
Процитировано
2
Frontiers in Pharmacology, Год журнала: 2024, Номер 15
Опубликована: Окт. 4, 2024
Background Human cancers, including head and neck squamous cell carcinoma (HNSCC), are complex heterogeneous diseases driven by uncontrolled growth proliferation. Post-translational modifications (PTMs) of proteins play a crucial role in cancer progression, making them promising target for pharmacological intervention. This study aims to identify key exercise-related genes with prognostic value HNSCC through comprehensive bioinformatics analysis, particular focus on the therapeutic potential placental factor (PIGF). Methods Transcriptome data were obtained from The Cancer Genome Atlas (TCGA) database. Differently expressed (DEGs) identified analyzed their significance. Exercise-related gene sets retrieved Gene Set Enrichment Analysis (GSEA) Functional enrichment analyses, Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG), GSEA, conducted. biological functions clinical implications further explored single-gene expression immune infiltration vitro cellular experiments. Results associated survival prognosis HNSCC. GO KEGG pathway analyses highlighted these genes, Kaplan-Meier curves confirmed value. PIGF analysis using TCGA showed its diagnostic potential, higher linked advanced tumor stages. Single-cell sequencing revealed PIGF’s microenvironment. In experiments demonstrated that plays pivotal enhancing proliferation colony formation HNSCC, knockdown significantly impairing functions, highlighting importance regulation. Additionally, predictive performance drug sensitivity across datasets suggests as target, offering opportunities modulate microenvironment improve outcomes treatment. Conclusion provides new insights into molecular mechanisms underlying identifies particularly PIGF, biomarkers treatment personalized medicine. By focusing PTMs our findings suggest targeting may offer innovative strategies.
Язык: Английский
Процитировано
1
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 287, С. 138589 - 138589
Опубликована: Дек. 9, 2024
Breast cancer (BC) is one of the common female cancers and it characterized by considerable problems regarding its development therapy. Long non-coding RNAs (lncRNAs) have been identified as significant modulators in BC development, especially, tumorigenicity chemoresistance. We therefore endeavor to present an up-to-date understanding lncRNAs their impact on progression treatment, concerning molecular processes, treatment options, use a therapeutic opportunity. LncRNAs are novel regulators genes that cause resistance directly functioning both coding patients, but little known about mechanisms actions. Thus, additional study required deeper modes action possible roles disease. This aims investigate functions BC, with particular attention role tumorigenesis, drug mechanisms, targets. will help identify targets improve effectiveness treatment.
Язык: Английский
Процитировано
1
Discover Oncology, Год журнала: 2024, Номер 15(1)
Опубликована: Июнь 28, 2024
Abstract The long noncoding DANCR functions as a tumor oncogene in many cancers, including colorectal cancer (CRC). However, the molecular mechanism of CRC has not been explored. This study probed function and potential by which contributes to progression CRC. obtained data indicated that is overexpressed tissues cell lines. Knockdown hindered proliferation, was mediated cyclin D1 CDK4. Bioinformatic analysis, luciferase reporter assays subcellular fractionation verified directly binds miR-508-5p. Moreover, acts miR-508-5p ceRNA regulate expression ATF1. In addition, upregulation attributed H3K27 acetylation at promoter region. conclusion, our confirmed activation lncRNA an oncogenic role provides therapeutic target for
Язык: Английский
Процитировано
0
Immunologic Research, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
Язык: Английский
Процитировано
0