Poly-γ-glutamic acid alleviates slow transit constipation by regulating aquaporin and gut microbes
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 10, 2025
Slow
transit
constipation
(STC)
is
a
prevalent
gastrointestinal
disorder
caused
by
colon
dysfunction.
Poly-γ-glutamic
acid
(γ-PGA),
an
anionic
polymer
known
for
its
moisture
retention,
degradability,
and
food
safety,
was
studied
effects
on
loperamide-induced
STC
in
mice.
Treatment
with
γ-PGA
one
week
significantly
increased
both
defecation
frequency
fecal
water
content,
the
high-dose
group
(10
g/kg/d)
restoring
content
to
34.23%,
outperforming
low-
(16.16%)
medium-dose
(27.08%)
groups
exceeding
positive
control,
PEG,
1.35
times.
enhanced
intestinal
peristalsis
reduced
expression
of
inflammatory
markers
(IL-1β,
IL-6,
caspase-1,
TLR2)
water-electrolyte
transport
genes
(AQP3,
AQP4,
ENaC-β),
while
improving
tight
junction
proteins
(Claudin-1,
Occludin,
ZO-1)
damaged
loperamide.
Histopathological
analyses
confirmed
γ-PGA's
capacity
repair
damage.
Additionally,
Western
Blot
analysis
indicated
AQP3/4
levels
colon,
molecular
docking
showed
good
binding
affinity
between
AQPs.
also
positively
altered
gut
microbiota
composition.
Overall,
shows
promise
treating
modulating
aquaporins
microbiota.
Язык: Английский
Supplementation of Miso to a Western-Type Diet Stimulates ILC3s and Decreases Inflammation in the Small Intestine
Nutrients,
Год журнала:
2024,
Номер
16(21), С. 3743 - 3743
Опубликована: Окт. 31, 2024
Western-type
diets
(WDs)
damage
the
intestinal
barrier
by
disrupting
gut
microbiota
composition
and
causing
inflammation,
leading
to
development
of
obesity,
type
2
diabetes,
non-alcoholic
fatty
liver
disease.
Short-chain
acids
(SCFAs)
are
produced
found
in
fermented
foods
can
stimulate
anti-inflammatory
action
3
innate
lymphoid
cells
(ILCS3s)
intestine.
This
study
hypothesised
that
supplementing
miso,
a
Japanese
food,
WD
could
increase
levels
SCFAs
thus
ILC3s,
decreasing
inflammation
intestine
protecting
integrity.
Язык: Английский