Biomolecules,
Год журнала:
2024,
Номер
14(10), С. 1206 - 1206
Опубликована: Сен. 25, 2024
Chronic
stress
is
a
common
cause
of
hair
loss,
involving
inflammatory
responses
and
changes
in
cellular
signaling
pathways.
This
study
explores
the
mechanism
action
SDF-1/CXCR4
axis
chronic
stress-induced
loss.
The
research
indicates
that
SDF-1
promotes
follicle
growth
through
PI3K/Akt
JAK/STAT
Transcriptome
sequencing
analysis
was
conducted
to
identify
differentially
expressed
genes
skin
normal
stressed
mice,
with
key
selected
machine
learning
protein-protein
interaction
network
established.
A
mouse
model
created,
injections
AMD3100
administered
observe
growth,
weight
changes,
behavioral
alterations
validate
activity.
Skin
concentrations
were
measured,
screened,
pathways
enriched.
Activation
assessed,
siRNA
technology
used
vitro
inhibit
expression
or
CXCR4.
promoted
activity,
combined
injection
weakening
this
effect.
activation
observed
group,
confirmed
by
Western
blot
immunofluorescence.
Silencing
siRNA-mediated
inhibition
reduced
cell
proliferation
migration
abilities.
mice
activating
pathways,
an
effect
reversible
AMD3100.
may
serve
as
potential
therapeutic
target
for
Allergology International,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
The
detection
of
drug-specific
activation
T
cells
in
the
lymphocyte
transformation
test
(LTT)
is
mainly
based
on
cell
proliferation
or
cytokine
secretion.
However,
LTT
presents
with
a
varying
sensitivity
and
specificity.
aim
our
study
was
to
analyse
genome
wide
gene
expression
PBMC
identify
drug
allergy-specific
regulation
patterns.
Additionally,
differences
related
allergy-inducing
type
allergy
(immediate/delayed)
were
investigated.
Blood
samples
from
40
patients
different
drugs
non-drug-allergic
controls
recruited.
isolated
stimulated
culprit
("LTT-platform").
Transcriptome
analyses
conducted
after
3.5
days.
concentration
IFN-γ
IL-5
culture
supernatants
measured
by
ELISA
6
transcriptome
revealed
an
patients.
Importantly,
corresponding
control
groups
these
genes
barely
even
opposingly
regulated.
It
also
shown
that
particular
cefuroxime
exerted
strong
effect
PBMC.
Quantitative
RT-PCR
selected
identified
CCL17,
CISH
CD25
specifically
upregulated
Notably,
upregulation
did
not
necessarily
correlate
outcome
controls.
Our
results
could
be
helpful
for
identification
one
panel
regulated
considering
patient
specific
parameters
like
drug.
World Allergy Organization Journal,
Год журнала:
2024,
Номер
17(9), С. 100946 - 100946
Опубликована: Авг. 20, 2024
Drug
provocation
tests
(DPTs)
are
also
used
in
some
patients
with
a
history
of
contrast
medium
(CM)-hypersensitivity
reaction.
Since
the
use
agents
requires
special
knowledge
that
is
present
radiology
but
not
necessarily
allergology,
this
overview
should
close
gaps.
The
literature,
and
package
inserts
industry
dealing
DPTs
hypersensitivity
reactions
was
analyzed
results
presented.
Historical
analyses
revealed
were
already
done
past,
called
pre-testing.
Due
to
disadvantages,
diagnostic
tool
abandoned.
A
few
years
later,
DPT
introduced
as
an
innovative
procedure.
has
3
main
disadvantages:
missing
standardization,
at
risk
(such
compromised
renal
function)
rarely
taken
into
account,
negative
does
exclude
subsequent
CM
(formerly
pre-testing)
well-known
method
for
diagnosing
CM-related
reactions.
disadvantages
diagnosis
outweigh
advantages,
we
propose
replacing
routine
contrast-enhanced
imaging
examination
radiology.
Fundamental and Clinical Pharmacology,
Год журнала:
2024,
Номер
38(5), С. 988 - 997
Опубликована: Апрель 8, 2024
Hypersensitivity
reactions
(HSR)
are
reported
for
the
macrolides,
lincosamides,
and
streptogramins
(MLS)
antibiotic
family.
Data
about
cross-reactivity
among
between
MLS
remain
scarce
or
controversial.
The
aim
of
this
study
was
to
provide
an
overview
hypersensitivity
cross-reactions
MLSs
based
on
data
extracted
from
French
National
Pharmacovigilance
Database
(FPVD).
Cases
HSR
January
1985
December
2019
were
FPVD
using
standardized
MedDRA
queries
(SMQ).
including
allergological
test
involving
multiple
giving
at
least
one
positive
result
included.
Of
8394
cases
reviewed,
149
mainly
involved
pristinamycin
(n
=
83;
53.2%)
spiramycin
31;
19.9%).
immediate
in
54
delayed
94
cases.
Skin
tests
represented
majority
performed
728;
84.7%),
followed
by
reintroduction
79;
9.2%).
Eighty-six
cross-reactivities
identified
62
(41.6%).
All
25
explorations
showed
cross-reactivities,
but
only
30/253
macrolides
(11.9%).
Cross-reactivities
three
observed
31/322
(9.6%)
explorations.
This
highlights
possibility
MLSs.
Dermatologists
allergologists
managing
patients
with
should
be
aware
a
risk
different
classes
perform
testing
before
recommending
alternative
antibiotic,
especially
severe
drug
Trimethoprim-sulfamethoxazole
(TMP-SMX)
is
a
non-β-lactam
antibiotic
commonly
used
in
pediatrics
to
treat
infections
and
as
prophylactic
medication.
Hypersensitivity
TMP-SMX
generally
non-immediate,
immediate
allergic
reactions,
including
anaphylaxis,
are
rare.
This
case
report
details
six-year-old
girl
who
experienced
anaphylaxis
TMP-SMX.
Her
skin
prick
test
with
was
negative,
but
she
developed
drug
provocation
(DPT).
An
additional
basophil
activation
(BAT)
for
the
combination
tablet
evaluated
confirmed
positive.
underscores
need
alternative
diagnostic
methods
like
BAT,
which
pose
lower
risk
than
DPT.
The
findings
suggest
that
BAT
could
offer
safer
approach,
though
more
studies
required
validate
its
use
allergy
diagnosis.
Introduction
Cefuroxime
allergy
may
present
as
a
delayed-type
reaction
or
an
immunoglobulin
(Ig)E-mediated
immediate-type
anaphylactic
reaction.
The
basophil
activation
test
(BAT)
is
diagnostic
tool
for
cefuroxime-induced
reactions,
whereas
the
lymphocyte
transformation
(LTT)
typically
applied
in
drug
allergy.
This
study
aimed
to
compare
results
of
BAT
and
LTT
15
patients
with
reactions
considered
confirmed.
pharmacoepidemiological
part
analyze
spontaneous
reports
cefuroxime-associated
European
adverse
database
(EudraVigilance).
Methods
In
EudraVigilance,
668
Economic
Area
(EEA)
between
2010
2023
were
analyzed,
182
(27.2%)
these
originating
from
Germany.
performed
according
standard
protocols.
Except
one
patient,
all
prior
skin
tests,
thereafter.
Results
Almost
classified
serious
(EEA,
99.3%;
Germany,
98.9%).
60.8%
66.9%
(Germany)
respective
information,
occurred
after
intravenous
administration.
was
12
(3/12
positive;
sensitivity
25%),
while
(7/15
46.7%).
Conclusions
Our
analysis
highlights
importance
almost
serious.
Neither
negative
nor
can
rule
out
sensitization
reactions.