Antisense RNA, the modified CRISPR-Cas9, and metal/metal oxide nanoparticles to inactivate pathogenic bacteria

Cellular Molecular and Biomedical Reports, Год журнала: 2021, Номер 1(2), С. 52 - 59

Опубликована: Дек. 1, 2021

Finding efficient therapeutic strategies to fight antibiotic-resistant bacteria is a complicated affair specifically in the therapy of chronic bacterial infections related hospital-acquired infections. Recently, three major antibacterial systems based on antisense RNA, CRISPR-Cas9, and metal/metal oxide nanoparticles particularly silver (Ag) have shown more effective activity compared conventional antibiotics. ROS generation, attachment cell membrane, disruption envelop, inactivation electron transport chain, decreasing local pH, modulation signaling, denaturation biological macromolecules such as proteins nucleic acids been found main functions Ag nanoparticles. Antisense single-stranded can hybridize with complementary genes messenger RNA (mRNA) followed by blockage translation these into proteins. Moreover, CRISPR (clustered regularly interspaced short palindromic repeats) family viral DNA sequences derived from bacteriophages, which target destroy foreign nuclease activity. There are 2 classes 6 subtypes (I-VI) CRISPR-Cas systems, may be engineered potential agents specific sequences. Therefore, here, recent advances challenges for application presented.

Язык: Английский

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Risk assessment of gastric cancer in the presence of Helicobacter pylori cagA and hopQII genes

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(4), С. 299 - 305

Опубликована: Янв. 2, 2022

Helicobacter pylori bacterium is one of the most common bacterial infections globally and leading cause indigestion, gastric duodenal ulcers, cancer. This can escape antibacterial effects stomach acid by adapting to inner layers stomach. It combines with natural sugars in mucosa. The compound so effective that it makes resistant. For genes related pathogenesis H. pylori, using existence such as cagA, hopQI, hopQII, PCR performed on some these amplify fragments different lengths. One less-studied cases two or more pathogenic are simultaneously associated pylori. study examined frequency diseases healthy individuals infected cagA hopQII genotypes. To diagnose infection cancer patients, products electrophoresed agarose gel after glmM gene amplification PCR. this aim, tissue biopsies were used for saliva was individuals. purpose, 150 biopsy samples from patients people collected. Data showed a significant relationship between coexistence genes, 34.2% 10.1% genotypes, while other (89.9%) did not have genotype. Therefore, simultaneous presence human societies be an essential biomarker diagnosis prognosis

Язык: Английский

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Nanoformulations of curcumin and quercetin with silver nanoparticles for inactivation of bacteria

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(5), С. 151 - 156

Опубликована: Фев. 4, 2022

Antibiotic resistance in pathogenic bacteria to various types of antibiotics has resulted the necessity new effective strategies get around this problem. In recent investigations, metal or oxide nanoparticles specifically silver (AgNPs) have been employed successfully hinder antibiotic-resistant Gram-negative and Gram-positive bacteria. However, AgNPs at high concentrations cytotoxicity for eukaryotic cells which, application other biocompatible materials particularly plant secondary metabolites curcumin quercetin reduce is a critical affair. These compounds may be used directly indirectly produce AgNPs. regard, modified NPs by shown an increased therapeutic effect biocompatibility biodegredibility properties. Therefore, here, advances challenges about antibacterial properties nanoformulation with are presented.

Язык: Английский

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Antimicrobial and wound healing activities of electrospun nanofibers based on functionalized carbohydrates and proteins

Cellulose, Год журнала: 2022, Номер 29(3), С. 1331 - 1347

Опубликована: Янв. 6, 2022

Язык: Английский

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A systematic ethnobotanical review of Adhatoda vasica (L.), Nees.

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(4), С. 248 - 263

Опубликована: Янв. 2, 2022

Adhatoda vasica (L.), Nees belonging to the family Acanthaceae is a shrub with opposite ascending branches. The plant has been used in indigenous system of medicine India for near about 2500 years. It well-known as drug Ayurvedic and Unani medicines. Traditionally it was treatment various acute chronic diseases showed strong pharmacological activity particularly bronchial infections, cough, bacterial reproductive disorders, cardiac many more. Various phytochemicals like alkaloids, flavonoids, tannins, etc. were obtained from (A. vasica). active constituent vasicine, l-vasicinone, deoxyvasicine, maiontone, vasicinolone vasicinol This review consists updated information on phyto-constituents isolated A. their potential role ailments traditionally medically. Based critical concluded that there not sufficient scientifically evidence explain extract, could be harmful human beings especially pregnant women. Major data traditional uses well toxicological studies, evaluated correctness, relevance, importance, reliability overall evaluation safety. Numerous clinical trials are conducted around globe herbal formulations vasaka. includes phytochemical ethnopharmacological studies indicate versatile native Indian subcontinent having commercial reputation thus can encouraged diversified applications medicinal other uses.

Язык: Английский

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Exploring the interactions of rapamycin with target receptors in A549 cancer cells: insights from molecular docking analysis

Journal of Cancer Research and Clinical Oncology, Год журнала: 2025, Номер 151(1)

Опубликована: Янв. 7, 2025

Язык: Английский

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Metal, metal oxide and polymeric nanoformulations for the inhibition of bacterial quorum sensing

Drug Discovery Today, Год журнала: 2022, Номер 28(1), С. 103392 - 103392

Опубликована: Окт. 5, 2022

Antibiotic resistance of bacteria has caused a significant public health challenge and economic problem, resulting in necessity to find efficient antibacterial agents. Conventional bactericidal agents hinder the growth by slowing down cell wall synthesis or disturbing bacterial DNA replication, protein production other cellular metabolism that can augment natural selection pressure for turning up new antibiotic-resistant strains. Virulence properties biofilm formation are orchestrated quorum-sensing systems. These systems normally control antimicrobial production; targeting these using metal-based nanoparticles polymeric be considered as powerful treatments owing their specific physicochemical therapeutic properties. In this review, recent advances challenges related inactivation presented obtain comprehensive viewpoints future studies.

Язык: Английский

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Chemical and green synthesis of gold nanoparticles for mRNA delivery in vitro

Advances in Natural Sciences Nanoscience and Nanotechnology, Год журнала: 2024, Номер 15(1), С. 015009 - 015009

Опубликована: Март 1, 2024

Abstract Nanotechnology has paved the way for novel treatment strategies diseases such as cancer. While chemical synthesis of nanoparticles (NPs) can introduce toxic and expensive reducing reagents, green offers an alternative with dual capping properties. Curcumin exhibits favourable pharmacological properties, enabling synergism therapeutic cargo. This study aimed to compare chemically synthesised gold NPs delivery F Luc -mRNA in vitro . Chemical AuNPs were produced using trisodium citrate curcumin, respectively, functionalised poly-L-lysine (PLL) polyethylene glycol (PEG). The NP:mRNA nanocomplexes characterised nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV–visible spectroscopy. Various mRNA binding studies assessed optimal binding, compaction, protection mRNA. All small (<150 nm) had good colloidal stability, protection. MTT (3-[4,5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) assay showed cell viability, significant transgene expression noted luciferase reporter gene assay. Higher transfection was achieved human cervical carcinoma (HeLa) than breast adenocarcinoma (MCF-7) embryonic kidney (HEK293) cells. Both curcumin-synthesised displayed similar activity all cells, curcumin-capped marginally better at same concentration.

Язык: Английский

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Antibacterial and wound healing applications of curcumin in micro and nano-scaffolds based on chitosan, cellulose, and collagen

Cellular and Molecular Biology, Год журнала: 2022, Номер 68(3), С. 9 - 14

Опубликована: Март 31, 2022

About 80% higher risk of amputation resulted from microbial infection was indicated for patients with diabetic foot ulcers (DFUs). Micro and nano-scaffolds made natural polymers specifically cellulose, chitosan, collagen can donate the biocompatibility, biodegradability, bioavailability properties appropriate to accelerate wound closure before biofilm formation. Antimicrobial activity these dressings be improved by incorporation bioactive compounds extracted medicinal plant species such as curcumin. Low water solubility poor are recognized two main disadvantages curcumin, lipophilic phytopolyphenol, which could controlled targeted polymeric micro nano-scaffolds. Consequently, this review has discussed capacity challenges types formulations according recent investigations.

Язык: Английский

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Curcumin reduces inflammation in mice with the psoriasis model by inhibiting NLRP3 inflammatory bodies

Cellular and Molecular Biology, Год журнала: 2022, Номер 67(6), С. 48 - 54

Опубликована: Фев. 27, 2022

As a chronic skin disease, psoriasis is relatively common disease among various types of diseases. Because this often distributed throughout the patient's body and prone to develop, it difficult guarantee quality life physical mental health patients with disease. The purpose article investigate whether curcumin can effectively inhibit NLRP3 inflammatory thereby reduce inflammation in mouse model. Through use gel prepared model, percutaneous administration was used mechanism curcumin's effect on reducing In addition, order better explore curative psoriasis, related experiments were conducted by setting up control group an experimental group. results show that has good inhibitory bodies. Curcumin not only expression caused IL-22 IL-18 but also damage psoriasis. 22 Induced phosphorylation STAT3 almost completely inhibits normal cells. Among them, inhibited IL-22-induced 95.6%, about 47%.

Язык: Английский

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