Drug Metabolism and Bioanalysis Letters,
Год журнала:
2024,
Номер
17(2), С. 56 - 66
Опубликована: Окт. 8, 2024
Background:
This
study
developed
a
UV
spectrophotometric
method
for
quantifying
allopurinol
and
lycopene
in
both
bulk
dosage
forms,
aiming
simplicity
practicality.
Materials
Methods:
The
was
validated
using
methanol
lyco-pene
phosphate
buffer
saline
at
various
pH
levels
(7.4,
6.8,
1.2,
5.5).
Validation
parameters
included
linearity,
accuracy,
precision,
limit
of
quantification
(LOQ),
detection
(LOD)
according
to
ICH
standards.
Results:
demonstrated
high
sensitivity
linearity
over
concentration
range
4-20
μg/ml,
adhering
Beer's
law.
LOQ
LOD
were
2-18
μg/ml
2-6
respectively.
Optimal
absorbance
wavelengths
251
nm
471
allopurinol.
Calibration
curves
showed
correlation
coefficient
(0.9994),
indicating
strong
linear
relationship.
Conclusion:
is
effective,
interference-free,
suitable
routine
quality
control,
providing
reliable
measurements
lyco-pene.
Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Фев. 26, 2025
Intravenous
leiomyomatosis
(IVL)
is
a
histologically
well
differentiated
smooth
muscle
tumor
with
aggressive
behavior,
capable
of
extending
throughout
the
venous
system.
Understanding
how
IVL
occurs
and
develops
really
important
for
diagnosing
treating
it.
Unfortunately,
because
quite
rare,
there
aren't
many
comprehensive
studies
available.
In
our
research,
we
carried
out
an
extensive
multi-omics
study,
gathering
tissue
samples
from
cases,
uterine
fibroid,
normal
myometrium.
The
single-cell
RNA
sequencing
analysis
revealed
notable
difference
in
cell
composition
between
fibroid.
Additionally,
H&E
staining
demonstrated
more
frequent
hydropic
changes
hyalinization
tissues,
along
reduced
vascular
density
compared
to
both
myometrium
proteomics
eight
paired
fresh
frozen
tissue,
identified
proteins
that
were
differentially
expressed,
mainly
related
focal
adhesions
regulation
actin
cytoskeleton.
most
frequently
involved
chromosomes
included
deletions
10q22.2,
10q24.32,
13q14,
13q21-31.
Correlation
analyses
highlighted
chromosome
10q
as
cytoband,
corresponding
regulating
Integrated
pathological
clinical
characteristics
indicated
deletion
morphology
could
serve
markers
predicting
behavior.
Our
study
sheds
light
on
molecular
linked
IVL,
which
pave
way
new
treatment
approaches.
