Biomarker Research,
Год журнала:
2024,
Номер
12(1)
Опубликована: Окт. 14, 2024
Cell-derived
extracellular
vesicles
(EVs),
which
carry
diverse
biomolecules
such
as
nucleic
acids,
proteins,
metabolites,
and
lipids
reflecting
their
cell
of
origin,
are
released
under
both
physiological
pathological
conditions.
EVs
have
been
demonstrated
to
mediate
cell-to-cell
communication
serve
biomarkers.
EV-associated
DNA
(EV-DNA)
comprises
genomic
mitochondrial
(i.e.,
gDNA
mtDNA)
fragments.
Some
studies
revealed
that
EV-DNA
can
represent
the
full
nuclear
genome
parental
cells.
Furthermore,
fragments
loaded
into
stable
be
transferred
recipient
cells
regulate
biological
functions.
In
this
review,
we
summarized
discussed
research
advances
with
an
emphasis
on
detection
at
population-EV
single-EV
levels,
gene
transfer-associated
functions,
clinical
applications
biomarkers
for
disease
liquid
biopsy.
We
hope
review
will
provide
potential
directions
or
guidance
future
investigations.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 23, 2024
Abstract
Wound
healing
is
an
obvious
clinical
concern
that
can
be
hindered
by
inadequate
angiogenesis,
inflammation,
and
chronic
hypoxia.
While
exosomes
derived
from
adipose
tissue-derived
stem
cells
have
shown
promise
in
accelerating
carrying
therapeutic
growth
factors
microRNAs,
intracellular
cargo
delivery
compromised
hypoxic
tissues
due
to
activated
hypoxia-induced
endocytic
recycling.
To
address
this
challenge,
we
developed
a
strategy
coat
oxygen
nanobubbles
with
incorporate
them
into
polyvinyl
alcohol/gelatin
hybrid
hydrogel.
This
approach
not
only
alleviates
wound
hypoxia
but
also
offers
efficient
means
of
delivering
exosome-coated
nanoparticles
conditions.
The
self-healing
properties
the
hydrogel,
along
its
component,
gelatin,
aids
hemostasis,
while
crosslinking
bonds
facilitate
hydrogen
peroxide
decomposition,
ameliorate
inflammation.
Here,
show
potential
multifunctional
hydrogel
for
enhanced
healing,
promoting
facilitating
exosome
delivery,
mitigating
hypoxia,
inhibiting
inflammation
male
rat
full-thickness
model.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
11
Опубликована: Дек. 12, 2023
The
intricate
healing
process
of
skin
wounds
includes
a
variety
cellular
and
molecular
events.
Wound
heavily
relies
on
reactive
oxygen
species
(ROS),
which
are
essential
for
controlling
various
processes,
including
inflammation,
cell
growth,
angiogenesis,
granulation,
the
formation
extracellular
matrix.
Nevertheless,
an
overabundance
(ROS)
caused
by
extended
oxidative
pressure
may
result
in
postponement
or
failure
wound
healing.
It
is
crucial
to
comprehend
function
create
biomaterials
that
efficiently
eliminate
ROS
enhance
wounds.
In
this
study,
thorough
examination
presented
role
healing,
along
with
exploration
existing
knowledge
regarding
employed
elimination.
addition,
article
covers
different
techniques
substances
used
management
wound.
future
prospects
clinical
applications
enhanced
also
emphasized,
highlighting
potential
scavenge
active
promote
repair.
This
seeks
understanding
complex
processes
application
ROS-scavenging
materials.
Its
objective
novel
strategies
effective
treatment
Abstract
The
healing
process
at
a
wound
is
made
up
of
many
types
cells,
growth
factors,
the
extracellular
matrix,
nerves
and
blood
vessels
all
interacting
with
each
other
in
complex
changing
ways.
Microbial
colonization
proliferation
are
possible
place
injury,
which
makes
infection
more
likely.
Because
this,
any
cut
has
chance
getting
an
infection.
Researchers
have
found
that
infections
make
patients
upset
cost
healthcare
system
lot
money.
Surgical
site
happen
to
people
who
recently
had
surgery.
This
study
shows
such
surgical
linked
high
rate
illness
death.
shown
by
fact
25%
get
serious
sepsis
need
be
transferred
intensive
care
unit.
In
both
animal
models
people,
mesenchymal
stem
cells
(MSCs)
play
active
role
stages
positive
effects.
Exosomes
one
main
things
MSCs
release.
They
effects
similar
those
parent
MSCs.
Various
effector
proteins,
messenger
RNA
microRNAs
can
transported
vesicles
control
activity
target
cells.
big
impact
on
process.
These
results
suggest
using
MSC-exosomes
as
new
type
cell-free
therapy
could
better
safer
option
than
whole
cell
therapy.
review
mostly
about
how
use
parts
help
heal.
Theranostics,
Год журнала:
2024,
Номер
14(5), С. 2099 - 2126
Опубликована: Янв. 1, 2024
Exosomes,
which
are
small
vesicles
enclosed
by
a
lipid
bilayer
and
released
many
cell
types,
widely
dispersed
have
garnered
increased
attention
in
the
field
of
regenerative
medicine
due
to
their
ability
serve
as
indicators
diseases
agents
with
therapeutic
potential.
Exosomes
play
crucial
role
mediating
intercellular
communication
through
transfer
biomolecules,
including
proteins,
lipids,
RNA,
other
molecular
constituents,
between
cells.
The
targeted
transport
proteins
nucleic
acids
specific
cells
has
potential
enhance
or
impair
biological
functions.
applications,
they
can
be
used
alone
combination
approaches.
examination
unique
attributes
functions
these
factors
emerged
prominent
study
realm
biomedical
research.
This
manuscript
summarizes
origins
properties
exosomes,
structural,
biological,
physical,
chemical
aspects.
paper
offers
complete
recent
progress
tissue
repair
medicine,
emphasizing
possible
implications
methods
forthcoming
regeneration
attempts.
Abstract
Diabetic
wounds
have
imposed
a
significant
burden
on
both
patients
and
society
with
prolonged
healing
processes
hindered
by
dysfunctional
skin
repair
cells.
Small
extracellular
vesicles
(sEVs),
as
the
important
media
for
intercellular
communications,
show
promising
therapeutic
potential
in
treating
diabetic
restoring
cellular
functions.
However,
low
yields
limited
bio‐function
of
sEVs
greatly
challenge
their
large‐scale
clinical
use.
Here,
we
briefly
overview
biogenesis
uptake
sEVs,
emphasize
current
advances
improving
optimizing
function
engineering
approaches,
summarize
applications
engineered
wound
treatment.
Furthermore,
undissolved
issues
during
transformation
are
also
discussed.
This
critical
review
aims
to
provide
meaningful
guidance
future
management
wounds.
Pharmaceutics,
Год журнала:
2024,
Номер
16(6), С. 709 - 709
Опубликована: Май 24, 2024
Of
all
the
numerous
nanosized
extracellular
vesicles
released
by
a
cell,
endosomal-originated
exosomes
are
increasingly
recognized
as
potential
therapeutics,
owing
to
their
inherent
stability,
low
immunogenicity,
and
targeted
delivery
capabilities.
This
review
critically
evaluates
transformative
of
exosome-based
modalities
across
pharmaceutical
precision
medicine
landscapes.
Because
precise
biomolecular
cargo
delivery,
posited
ideal
candidates
in
drug
enhancing
regenerative
strategies,
advancing
diagnostic
technologies.
Despite
significant
market
growth
projections
exosome
therapy,
its
utilization
is
encumbered
substantial
scientific
regulatory
challenges.
These
include
lack
universally
accepted
protocols
for
isolation
complexities
associated
with
navigating
environment,
particularly
guidelines
set
forth
U.S.
Food
Drug
Administration
(FDA).
presents
comprehensive
overview
current
research
trajectories
aimed
at
addressing
these
impediments
discusses
prospective
advancements
that
could
substantiate
clinical
translation
exosomal
therapies.
By
providing
analysis
both
capabilities
hurdles
therapeutic
applications,
this
article
aims
inform
direct
future
paradigms,
thereby
fostering
integration
systems
into
mainstream
practice.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Burn
care
and
treatment
differ
markedly
from
other
types
of
wounds,
as
they
are
significantly
more
prone
to
infections
struggle
maintain
fluid
balance
post-burn.
Moreover,
the
limited
self-healing
abilities
exacerbate
likelihood
scar
formation,
further
complicating
recovery
process.
To
tackle
these
issues,
an
asymmetric
wound
dressing
comprising
a
quercetin-loaded
poly(3-hydroxybutyrate-co-4-hydroxybutyrate)
(P34HB@Qu)
hydrophilic
layer
zinc
oxide
nanoparticle-loaded,
thermally
treated
polyvinylidene
fluoride
(HPVDF@ZnO)
hydrophobic
is
designed.
This
provided
antibacterial
property
exudate
management
in
early
stages
burn
treatment,
preventing
infection
maintaining
moisture
at
site.
As
healing
progresses,
electroactive
properties
HPVDF@ZnO
quercetin
P34HB@Qu
synergistically
regulate
cell
migration
differentiation,
accelerating
facilitating
scar-free
regeneration.
Furthermore,
assisted
regeneration
skin
appendages.
study
underscores
full-cycle
strategy
versatile
dressings
for
spatiotemporal
injury
scarless
healing.
Journal of Inflammation Research,
Год журнала:
2023,
Номер
Volume 16, С. 1905 - 1921
Опубликована: Май 1, 2023
Abstract:
Burns
are
a
seriously
underestimated
form
of
trauma
that
not
only
damage
the
skin
system
but
also
cause
various
complications,
such
as
acute
kidney
injury
(AKI).
Recent
clinical
studies
have
shown
proportion
chronic
diseases
(CKD)
in
burn
patients
after
discharge
is
significantly
higher
than
general
population,
mechanism
behind
this
controversial.
The
traditional
view
CKD
associated
with
hypoperfusion,
AKI,
sepsis,
and
drugs
administered
early
stages
burns.
However,
recent
burns
can
long-term
immune
dysfunction,
which
high-risk
factor
for
CKD.
This
suggests
affect
kidneys
more
previously
recognized.
In
other
words,
severe
an
disease.
Neglecting
to
study
function
results
lack
preventive
therapeutic
methods
being
developed.
Furthermore,
stem
cells
their
exosomes
excellent
comprehensive
properties
prevention
treatment
CKD,
making
them
increasingly
focus
research
attention.
Their
engineering
strategy
further
improved
performance.
review
will
on
advances
development
illustrating
possible
burn-induced
introducing
potential
biological
options
strategies.
Keywords:
inflammation,
injury,
diseases,
cells,
exosomes,
cytokine
storm
Regenerative Biomaterials,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 1, 2024
Abstract
Adipose
mesenchymal
stem
cell
(ADMSC)-derived
exosomes
(ADMSC-Exos)
have
shown
great
potential
in
regenerative
medicine
and
been
evidenced
benefiting
wound
repair
such
as
burns.
However,
the
low
yield,
easy
loss
after
direct
coating,
no
suitable
loading
system
to
improve
their
availability
efficacy
hinder
clinical
application
for
healing.
And
few
studies
focused
on
comparison
of
biological
functions
between
derived
from
different
culture
techniques,
especially
exosome-releasing
hydrogel
system.
Therefore,
we
designed
a
high-performance
exosome
controllable
releasing
burn
healing,
namely
3D-printed
microfiber
culture-derived
highly
biocompatible
hyaluronic
acid
(HA).
In
this
project,
compared
vitro
model
among
conventional
two-dimensional
(2D)
plate
(2D-Exos),
microcarrier
(2.5D-Exos),
(3D-Exos).
Results
showed
that
with
2D-Exos
2.5D-Exos,
3D-Exos
promoted
HACATs
HUVECs
proliferation
migration
more
significantly.
Additionally,
had
stronger
angiogenesis-promoting
effects
tube
formation
(HUVECs)
cells.
Moreover,
found
HA-loaded
better
healing
promotion
including
accelerated
rate
collagen
remodeling.
The
study
findings
reveal
HA-loaded,
controllable-release
distinctly
augments
therapeutic
terms
while
concurrently
introducing
facile
approach.
This
markedly
bolsters
exosomal
efficiency,
provides
robust
protective
milieu,
potentiates
inherent
functionalities
exosomes.
Our
provide
rationale
efficient
utilization
high-quality
high-yield
3D
future,
novel
strategy
severe
