Targeting ABCA1 via Extracellular Vesicle‐Encapsulated Staurosporine as a Therapeutic Strategy to Enhance Radiosensitivity DOI
Qi Yang, Wenyan Gao, Xinyue Li

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(16)

Опубликована: Март 12, 2024

Abstract Cancer stem cells (CSCs) are essential for tumor initiation, recurrence, metastasis, and resistance. However, targeting CSCs as a therapeutic approach remains challenging. Here, stemness signature based on 22‐gene is developed to predict prognosis in esophageal squamous cell carcinoma (ESCC). Staurosporine (STS) identified radioresistance suppressor by high‐throughput screening of library 2131 natural compounds, leading dramatically improved radiotherapy efficacy subcutaneous models. Mechanistically, STS inhibits proliferation through the mTOR/AKT signaling pathway suppressed ATP‐binding cassette A1 (ABCA1), which transcriptionally regulated liver X receptor alpha (LXRα). can selectively bind nucleotide‐binding domain (NBD) ABCA1 compete ATP, blocking ABCA1‐mediated drug efflux facilitating intracellular accumulation STS. Considering cytotoxicity STS, an extracellular vesicle‐encapsulated system (EV‐STS) established effective delivery. EV‐STS shows remarkable growth inhibition, even at half dose with superior safety efficacy. These findings indicate that may serve predictor response neoadjuvant chemotherapy and/or ESCC patients. has shown antitumor low systemic toxicity, offering promising ESCC.

Язык: Английский

Modulating Effects of Cancer-Derived Exosomal miRNAs and Exosomal Processing by Natural Products DOI Open Access

Ya-Ting Chuang,

Jen‐Yang Tang, Jun‐Ping Shiau

и другие.

Cancers, Год журнала: 2023, Номер 15(1), С. 318 - 318

Опубликована: Янв. 3, 2023

Cancer-derived exosomes exhibit sophisticated functions, such as proliferation, apoptosis, migration, resistance, and tumor microenvironment changes. Several clinical drugs modulate these exosome but the impacts of natural products are not well understood. Exosome functions regulated by processing, secretion assembly. The modulation exosome-processing genes can exert anticancer precancer effects cancer-derived exosomes. This review focuses on exosomal miRNAs that regulate acting natural-product-modulating cell cancer cells. However, role processing has been overlooked in several studies products. In this study, utilizing bioinformatics database (miRDB), natural-product-modulated were predicted. Consequently, described here. sheds light improves our understanding modulating their potential targets treatments based use

Язык: Английский

Процитировано

9
Recent Progress in Extracellular Vesicle-Based Carriers for Targeted Drug Delivery in Cancer Therapy DOI Creative Commons

Yaqin Tang,

Xingyou Liu, Meng Sun

и другие.

Pharmaceutics, Год журнала: 2023, Номер 15(7), С. 1902 - 1902

Опубликована: Июль 7, 2023

Extracellular vesicles (EVs) are small, membrane-based released by cells that play a critical role in various physiological and pathological processes. They act as vehicles for transporting variety of endogenous cargo molecules, enabling intercellular communication. Due to their natural properties, EVs have emerged promising “cell-free therapy” strategy treating diseases, including cancer. serve excellent carriers different therapeutics, nucleic acids, proteins, small other nanomaterials. Modifying or engineering can improve the efficacy, targeting, specificity, biocompatibility EV-based therapeutics cancer therapy. In this review, we comprehensively outline biogenesis, isolation, methodologies EVs, well biological functions. We then focus on specific applications drug therapy citing prominent recent studies. Additionally, discuss opportunities challenges using pharmaceutical delivery vehicles. Ultimately, aim provide theoretical technical support development treatment.

Язык: Английский

Процитировано

9
From biogenesis to aptasensors: advancements in analysis for tumor-derived extracellular vesicles research DOI Creative Commons

Gaojian Yang,

Zhiyang Li, Rabia Usman

и другие.

Theranostics, Год журнала: 2024, Номер 14(10), С. 4161 - 4183

Опубликована: Янв. 1, 2024

Extracellular vesicles (EVs) are enclosed by a nanoscale phospholipid bilayer membrane and typically range in size from 30 to 200 nm.They contain high concentration of specific proteins, nucleic acids, lipids, reflecting but not identical the composition parent cell.The inherent characteristics variety EVs give them extensive unique advantages field cancer identification treatment.Recently, have been recognized as potential tumor markers for detection cancer.Aptamers, which molecules single-stranded DNA or RNA, demonstrate remarkable specificity affinity their targets adopting distinct tertiary structures.Aptamers offer various over protein counterparts, such reduced immunogenicity, ability convenient large-scale synthesis, straightforward chemical modification.In this review, we summarized biogenesis, sample collection, isolation, storage characterization, finally provided comprehensive survey analysis techniques that based on aptamers.

Язык: Английский

Процитировано

3
Hydrogels in Gene Delivery Techniques for Regenerative Medicine and Tissue Engineering DOI

Kexing Xu,

Qinmeng Zhang,

Danji Zhu

и другие.

Macromolecular Bioscience, Год журнала: 2024, Номер 24(6)

Опубликована: Янв. 24, 2024

Hydrogels are 3D networks swollen with water. They biocompatible, strong, and moldable emerging as a promising biomedical material for regenerative medicine tissue engineering to deliver therapeutic genes. The excellent natural extracellular matrix simulation properties of hydrogels enable them be co-cultured cells or enhance the expression viral non-viral vectors. Its biocompatibility, high strength, degradation performance also make action process carriers in tissues more ideal, making it an ideal material. It has been shown that hydrogel-based gene delivery technologies have potential play therapy-relevant roles organs such bone, cartilage, nerve, skin, reproductive organs, liver animal experiments preclinical trials. This paper reviews recent articles on explains manufacture, applications, developmental timeline, limitations, future directions techniques.

Язык: Английский

Процитировано

2
Targeting ABCA1 via Extracellular Vesicle‐Encapsulated Staurosporine as a Therapeutic Strategy to Enhance Radiosensitivity DOI
Qi Yang, Wenyan Gao, Xinyue Li

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(16)

Опубликована: Март 12, 2024

Abstract Cancer stem cells (CSCs) are essential for tumor initiation, recurrence, metastasis, and resistance. However, targeting CSCs as a therapeutic approach remains challenging. Here, stemness signature based on 22‐gene is developed to predict prognosis in esophageal squamous cell carcinoma (ESCC). Staurosporine (STS) identified radioresistance suppressor by high‐throughput screening of library 2131 natural compounds, leading dramatically improved radiotherapy efficacy subcutaneous models. Mechanistically, STS inhibits proliferation through the mTOR/AKT signaling pathway suppressed ATP‐binding cassette A1 (ABCA1), which transcriptionally regulated liver X receptor alpha (LXRα). can selectively bind nucleotide‐binding domain (NBD) ABCA1 compete ATP, blocking ABCA1‐mediated drug efflux facilitating intracellular accumulation STS. Considering cytotoxicity STS, an extracellular vesicle‐encapsulated system (EV‐STS) established effective delivery. EV‐STS shows remarkable growth inhibition, even at half dose with superior safety efficacy. These findings indicate that may serve predictor response neoadjuvant chemotherapy and/or ESCC patients. has shown antitumor low systemic toxicity, offering promising ESCC.

Язык: Английский

Процитировано

2