Bioengineering,
Год журнала:
2023,
Номер
10(4), С. 423 - 423
Опубликована: Март 27, 2023
The
human
fallopian
tube
epithelium
(hFTE)
is
the
site
of
fertilization,
early
embryo
development,
and
origin
most
high-grade
serous
ovarian
cancers
(HGSOCs).
Little
known
about
content
functions
hFTE-derived
small
extracellular
vesicles
(sEVs)
due
to
limitations
biomaterials
proper
culture
methods.
We
have
established
a
microfluidic
platform
hFTE
for
EV
collection
with
adequate
yield
mass
spectrometry-based
proteomic
profiling,
reported
295
common
sEV
proteins
first
time.
These
are
associated
exocytosis,
neutrophil
degranulation,
wound
healing,
some
crucial
fertilization
processes.
In
addition,
by
correlating
protein
profiles
tissue
transcripts
characterized
using
GeoMx®
Cancer
Transcriptome
Atlas,
spatial
transcriptomics
analysis
revealed
cell-type-specific
that
encode
sEVs
proteins,
among
which,
FLNA,
TUBB,
JUP,
FLNC
were
differentially
expressed
in
secretory
cells,
precursor
cells
HGSOC.
Our
study
provides
insights
into
establishment
baseline
profile
derived
from
tissue,
its
correlation
lineage-specific
transcripts,
which
can
be
used
evaluate
whether
shifts
cargo
during
cancer
carcinogenesis
role
reproductive
functions.
Journal of Extracellular Vesicles,
Год журнала:
2024,
Номер
13(7)
Опубликована: Июль 1, 2024
Abstract
Extracellular
vesicles
(EVs)
are
shed
from
the
plasma
membrane,
but
regulation
and
function
of
these
EVs
remain
unclear.
We
found
that
oxidative
stress
induced
by
H
2
O
in
Hela
cells
stimulated
filopodia
formation
secretion
EVs.
were
small
(150
nm)
labeled
for
CD44,
indicating
they
derived
filopodia.
Filopodia‐derived
(sEVs)
enriched
with
sphingolipid
ceramide,
consistent
increased
ceramide
membrane
Ceramide
was
colocalized
neutral
sphingomyelinase
(nSMase2)
acid
(ASM),
two
sphingomyelinases
generating
at
membrane.
Inhibition
nSMase2
ASM
prevented
stress‐induced
sEV
shedding
only
inhibition
formation.
S‐palmitoylated
interacted
to
generate
shedding.
sEVs
contained
decreased
level
enzymes
oxidatively
stressed
cells.
A
novel
metabolic
labeling
technique
showed
fluorescent
NBD‐ceramide.
NBD‐ceramide‐labeled
transported
mitochondria,
ultimately
inducing
cell
death
a
proportion
neuronal
(N2a)
In
conclusion,
using
we
provide
evidence
induces
interaction
filopodia,
which
leads
ceramide‐rich
target
mitochondria
propagate
death.
Applied Materials Today,
Год журнала:
2024,
Номер
37, С. 102084 - 102084
Опубликована: Фев. 3, 2024
Extracellular
vesicles
(EVs)
are
biologically
active
nanoparticles
secreted
by
cells,
which
have
a
variety
of
physiological
functions,
such
as
promoting
cell
proliferation,
regulating
immunity,
anti-inflammation,
and
facilitating
angiogenesis.
However,
the
application
EVs
is
limited
defects
low
therapeutic
efficiency,
lack
targeting,
unstable
effects.
In
recent
years,
various
engineering
methods
been
developed
to
enhance
function
EVs.
addition,
research
on
isolation
mechanisms
has
also
made
significant
progress.
this
paper,
we
review
biogenesis,
composition,
techniques
EVs,
summarize
potential
natural
well
strategies
for
EV
modification,
highlight
advances
in
tissue
regeneration.
Finally,
discuss
EVs'
current
problems
future
perspectives
regenerative
medicine.
Aging and Disease,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Although
significant
progress
has
been
made
in
early
lung
cancer
screening
over
the
past
decade,
it
remains
one
of
most
prevalent
and
deadliest
forms
worldwide.
Exosomal
proteomics
emerged
as
a
transformative
field
research,
with
potential
to
redefine
diagnostics,
prognostic
assessments,
therapeutic
strategies
through
lens
precision
medicine.
This
review
discusses
recent
advances
exosome-related
proteomic
glycoproteomic
technologies,
highlighting
their
revolutionise
treatment
by
addressing
issues
heterogeneity,
integrating
multiomics
data,
utilising
advanced
analytical
methods.
While
these
technologies
show
promise,
there
are
obstacles
overcome
before
they
can
be
widely
implemented,
such
need
for
standardization,
gaps
clinical
application,
importance
dynamic
monitoring.
Future
directions
should
aim
challenges
fully
utilize
exosomal
cancer.
promises
new
era
personalized
medicine
that
leverages
molecular
complexity
exosomes
groundbreaking
advancements
detection,
prognosis,
treatment.
Bioengineering,
Год журнала:
2023,
Номер
10(4), С. 423 - 423
Опубликована: Март 27, 2023
The
human
fallopian
tube
epithelium
(hFTE)
is
the
site
of
fertilization,
early
embryo
development,
and
origin
most
high-grade
serous
ovarian
cancers
(HGSOCs).
Little
known
about
content
functions
hFTE-derived
small
extracellular
vesicles
(sEVs)
due
to
limitations
biomaterials
proper
culture
methods.
We
have
established
a
microfluidic
platform
hFTE
for
EV
collection
with
adequate
yield
mass
spectrometry-based
proteomic
profiling,
reported
295
common
sEV
proteins
first
time.
These
are
associated
exocytosis,
neutrophil
degranulation,
wound
healing,
some
crucial
fertilization
processes.
In
addition,
by
correlating
protein
profiles
tissue
transcripts
characterized
using
GeoMx®
Cancer
Transcriptome
Atlas,
spatial
transcriptomics
analysis
revealed
cell-type-specific
that
encode
sEVs
proteins,
among
which,
FLNA,
TUBB,
JUP,
FLNC
were
differentially
expressed
in
secretory
cells,
precursor
cells
HGSOC.
Our
study
provides
insights
into
establishment
baseline
profile
derived
from
tissue,
its
correlation
lineage-specific
transcripts,
which
can
be
used
evaluate
whether
shifts
cargo
during
cancer
carcinogenesis
role
reproductive
functions.
