Non-coding RNAs in disease: from mechanisms to therapeutics

Nature Reviews Genetics, Год журнала: 2023, Номер 25(3), С. 211 - 232

Опубликована: Ноя. 15, 2023

Язык: Английский

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Noncoding RNAs: Master Regulator of Fibroblast to Myofibroblast Transition in Fibrosis

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 1801 - 1801

Опубликована: Янв. 16, 2023

Myofibroblasts escape apoptosis and proliferate abnormally under pathological conditions, especially fibrosis; they synthesize secrete a large amount of extracellular matrix (ECM), such as α-SMA collagen, which leads to the distortion organ parenchyma structure, an imbalance in collagen deposition degradation, replacement parenchymal cells by fibrous connective tissues. Fibroblast myofibroblast transition (FMT) is considered be main source myofibroblasts. Therefore, it crucial explore influencing factors regulating process FMT for prevention, treatment, diagnosis FMT-related diseases. In recent years, non-coding RNAs, including microRNA, long circular have attracted extensive attention from scientists due their powerful regulatory functions, been found play vital role FMT. this review, we summarized ncRNAs regulate during fibrosis that mainly regulated signaling pathways, TGF-β/Smad, MAPK/P38/ERK/JNK, PI3K/AKT, WNT/β-catenin. Furthermore, expression downstream transcription can promoted or inhibited, indicating potential new therapeutic target

Язык: Английский

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Expanding the Potential of Circular RNA (CircRNA) Vaccines: A Promising Therapeutic Approach

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 379 - 379

Опубликована: Янв. 4, 2025

In recent years, circular RNAs (circRNAs) have garnered significant attention due to their unique structure and function, positioning them as promising candidates for next-generation vaccines. The circRNA vaccine, an RNA offers advantages in preventing infectious diseases by serving a vector protein expression through non-canonical translation. Notably, vaccines demonstrated enduring antigenic generate larger percentage of neutralizing antibodies compared mRNA administered at the same dosage. Furthermore, can elicit robust cellular humoral immunity, indicating potential tumor vaccine development. However, certain challenges must be addressed facilitate widespread use both disease prevention treatment. These include low efficiency linear circularization, suboptimal targeting delivery systems, assessment side effects. This work aims describe characteristics functions circRNAs, elucidate mechanism behind vaccines, discuss applications treatment tumors, along with future applications.

Язык: Английский

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Interactions between circRNA and protein in breast cancer

Gene, Год журнала: 2023, Номер 895, С. 148019 - 148019

Опубликована: Ноя. 19, 2023

Язык: Английский

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METTL3-mediated m6A modification of NORAD inhibits the ferroptosis of vascular smooth muscle cells to attenuate the aortic dissection progression in an YTHDF2-dependent manner

Molecular and Cellular Biochemistry, Год журнала: 2024, Номер 479(12), С. 3471 - 3487

Опубликована: Фев. 22, 2024

Язык: Английский

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CircHIPK3 targets DRP1 to mediate hydrogen peroxide-induced necroptosis of vascular smooth muscle cells and atherosclerotic vulnerable plaque formation

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Апрель 1, 2024

Necroptosis triggered by H2O2 is hypothesized to be a critical factor in the rupture of atherosclerotic plaques, which may precipitate acute cardiovascular events. Nevertheless, specific regulatory molecules this development remain unclear. We aims elucidate mechanism from perspective circular RNA. There are few studies on circRNA VSMCs necroptosis. The objective our research shed light intricate roles that circHIPK3 plays process necroptosis and plaques prone rupture. Our study elucidates molecular mechanisms regulates vulnerable plaque formation through targeted proteins. Identifying at cellular level offers framework for understanding progression stability regulation, as well potential biomarker prognosis susceptible plaques. collected clinical vascular tissue HE staining Masson determine presence Then, NCBI database was used screen out with elevated expression tissue, up-regulated circRNA, circHIPK3, verified qRT-PCR FISH. Further, we synthesized circHIPK3′s small interference sequence overexpressed plasmid vitro, its regulation effect under physiological pathological conditions WB, PI staining. Through RNA pull down, mass spectrometry immunoprecipitation, DRP1 identified binding protein positively regulated circHIPK3. Meanwhile, basis silencing DRP1, mediated DRP1. Finally, validated ApoE-/- mice. investigated highly expressed increase promoted induced VSMCs. CircHIPK3 leading an elevation mitochondrial division rate, resulting increased reactive oxygen species impaired function, ultimately formation. interact involve Mitochondrial damage VSMCs, Silencing vivo can reduce findings have applications providing diagnostic biomarkers

Язык: Английский

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The role and mechanism of RNA-binding proteins in bone metabolism and osteoporosis

Ageing Research Reviews, Год журнала: 2024, Номер 96, С. 102234 - 102234

Опубликована: Фев. 16, 2024

Язык: Английский

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Epigenetic regulation of diverse regulated cell death modalities in cardiovascular disease: Insights into necroptosis, pyroptosis, ferroptosis, and cuproptosis

Redox Biology, Год журнала: 2024, Номер 76, С. 103321 - 103321

Опубликована: Авг. 19, 2024

Cell death constitutes a critical component of the pathophysiology cardiovascular diseases. A growing array non-apoptotic forms regulated cell (RCD)-such as necroptosis, ferroptosis, pyroptosis, and cuproptosis-has been identified is intimately linked to various conditions. These RCD are governed by genetically programmed mechanisms within cell, with epigenetic modifications being common crucial regulatory method. Such include DNA methylation, RNA histone acetylation, non-coding RNAs. This review recaps roles modifications, RNAs in diseases, well which regulate key proteins involved death. Furthermore, we systematically catalog existing pharmacological agents targeting novel their action article aims underscore pivotal role precisely regulating specific pathways thus offering potential new therapeutic avenues that may prove more effective safer than traditional treatments.

Язык: Английский

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The dual role of circHIPK3 in cancer and its implications for multiple drugs resistance: a systematic review and computational approach

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Фев. 21, 2025

circHIPK3 role in cancer as oncogene or tumor suppressor is still debated, therefore, this study aimed to understand the dual of circRNA different cancers. Furthermore, all available evidence interactions with sponged-miRNA and RBPs oncological diseases were systematically gathered better its functional cancer. PubMed, BioMedCentral, Web Science, Embase Scopus databases searched for articles published until October 2024, following PRISMA guideline. In computational analysis, miRNAs' sponged target genes used gene enrichment KEGG, REACTOME Gene Ontology, TISSUES expression. miRTargetLink 2.0 was search genes, STRING v.12.0 enrichment. can regulate 33 miRNAs which 399 that mainly enriched major biological pathways important development promoting. circHIPK3/miR-124-3p/miR-637/miR-338-3p are most well documented cancers may control MAPK, Jak/STAT3, Wnt/β-catenin, PI3K/Akt signaling pathways. regulates modulate responsible chemoresistance, such ATP-binding cassette solute carrier transporters DNA repair genes. has binding sites RBPs, participate RNA processing control, expression regulation. Finally, we believe it an onco-circRNA cancers, except bladder cancer, where a TS-circRNA function likely due microenvironment permeated by high amounts hydrogen peroxide. dysregulation mechanism establishment, progression chemoresistance making interesting molecule potential therapeutic target.

Язык: Английский

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Silencing circATXN1 in Aging Nucleus Pulposus Cell Alleviates Intervertebral Disc Degeneration via Correcting Progerin Mislocalization

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Circular RNAs (circRNAs) play a critical regulatory role in degenerative diseases; however, their functions and therapeutic applications intervertebral disc degeneration (IVDD) have not been explored. Here, we identified that novel circATXN1 highly accumulates aging nucleus pulposus cells (NPCs) accountable for IVDD. CircATXN1 accelerates cellular senescence, disrupts extracellular matrix organization, inhibits mitochondrial respiration. Mechanistically, circATXN1, regulated by heterogeneous nuclear ribonucleoprotein A2B1-mediated splicing circularization, promotes progerin translocation from the cell to cytoplasm expression of insulin-like growth factor 1 receptor (IGF-1R). To demonstrate potential siRNA targeting backsplice junction circATNX1 was screened delivered tetrahedral framework nucleic acids (tFNAs) due unique compositional structural features. Our delivery system demonstrates superior abilities transfect cells, clear intracellular ROS, enhanced biological safety. Using siRNA–tFNAs silence NPCs exhibit reduced mislocalization up-regulation IGF-1R, thereby demonstrating rejuvenated phenotype improved function. In vivo, administering an cell-adapted acid pathologically expressed premature mice (zmpste24 −/− ) can ameliorate tissue, effectively delaying This study only functioning as senescence promoter IVDD first time, but also successfully demonstrated its via tFNA-based strategy.

Язык: Английский

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