A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster

Cell, Год журнала: 2018, Номер 174(3), С. 730 - 743.e22

Опубликована: Июль 1, 2018

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping synaptic connectivity; however, the fly too conventional EM. We developed custom high-throughput EM platform imaged entire an adult female at resolution. To validate dataset, we traced brain-spanning circuitry involving mushroom body (MB), which been extensively studied its role in learning. All inputs to Kenyon cells (KCs), intrinsic neurons MB, were mapped, revealing previously unknown cell type, postsynaptic partners KC dendrites, unexpected clustering olfactory projection neurons. These reconstructions show that this freely available volume supports circuits, will significantly accelerate neuroscience. VIDEO ABSTRACT.

Язык: Английский

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The complete connectome of a learning and memory centre in an insect brain

Nature, Год журнала: 2017, Номер 548(7666), С. 175 - 182

Опубликована: Авг. 1, 2017

Язык: Английский

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Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Год журнала: 2019, Номер 179, С. 101607 - 101607

Опубликована: Март 22, 2019

Язык: Английский

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The connectome of an insect brain

Science, Год журнала: 2023, Номер 379(6636)

Опубликована: Март 10, 2023

Brains contain networks of interconnected neurons and so knowing the network architecture is essential for understanding brain function. We therefore mapped synaptic-resolution connectome an entire insect ( Drosophila larva) with rich behavior, including learning, value computation, action selection, comprising 3016 548,000 synapses. characterized neuron types, hubs, feedforward feedback pathways, as well cross-hemisphere brain-nerve cord interactions. found pervasive multisensory interhemispheric integration, highly recurrent architecture, abundant from descending neurons, multiple novel circuit motifs. The brain’s most circuits comprised input output learning center. Some structural features, multilayer shortcuts nested loops, resembled state-of-the-art deep architectures. identified provides a basis future experimental theoretical studies neural circuits.

Язык: Английский

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Integration of Parallel Opposing Memories Underlies Memory Extinction

Cell, Год журнала: 2018, Номер 175(3), С. 709 - 722.e15

Опубликована: Сен. 20, 2018

Accurately predicting an outcome requires that animals learn supporting and conflicting evidence from sequential experience. In mammals invertebrates, learned fear responses can be suppressed by experiencing predictive cues without punishment, a process called memory extinction. Here, we show extinction of aversive memories in Drosophila specific dopaminergic neurons, which indicate omission punishment is remembered as positive Functional imaging revealed co-existence intracellular calcium traces different places the mushroom body output neuron network for both original new appetitive memory. Light ultrastructural anatomy are consistent with parallel competing being combined within neurons direct avoidance. Indeed, extinction-evoked plasticity pair these neutralizes potentiated odor response imposed learning. Therefore, flies track accuracy expectations accumulating integrating events.

Язык: Английский

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Drosophila mushroom bodies integrate hunger and satiety signals to control innate food-seeking behavior

eLife, Год журнала: 2018, Номер 7

Опубликована: Март 16, 2018

The fruit fly can evaluate its energy state and decide whether to pursue food-related cues. Here, we reveal that the mushroom body (MB) integrates hunger satiety signals control food-seeking behavior. We have discovered five pathways in MB essential for hungry flies locate approach food. Blocking MB-intrinsic Kenyon cells (KCs) output neurons (MBONs) these impairs Starvation bi-directionally modulates MBON responses a food odor, suggesting controls occur at KC-to-MBON synapses. These are mediated by six types of dopaminergic (DANs). By manipulating DANs, could inhibit behavior or promote seeking fed flies. Finally, show DANs potentially receive multiple inputs signals. This work demonstrates an information-rich central circuit brain hunger-driven

Язык: Английский

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Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

Current Biology, Год журнала: 2020, Номер 30(16), С. 3183 - 3199.e6

Опубликована: Июль 2, 2020

Nervous systems contain sensory neurons, local projection and motor neurons. To understand how these building blocks form whole circuits, we must distil broad classes into neuronal cell types describe their network connectivity. Using an electron micrograph dataset for entire Drosophila melanogaster brain, reconstruct the first complete inventory of olfactory projections connecting antennal lobe, insect analog mammalian bulb, to higher-order brain regions in adult animal brain. We then connect this extant data literature, providing synaptic-resolution "holotypes" both heavily investigated previously unknown types. Projection neurons are approximately twice as numerous reported by light level studies; stereotyped, but not identical, synapse numbers between hemispheres. The lateral horn, cortical amygdala, is main target information has been shown guide innate behavior. Here, find new connectivity motifs, including axo-axonic feedback, inhibition axons a large population convergence different inputs, non-olfactory inputs memory-related feedback onto third-order These features less prominent mushroom body calyx, piriform cortex center associative memory. Our work provides neuroanatomical platform future studies system.

Язык: Английский

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Information flow, cell types and stereotypy in a full olfactory connectome

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 25, 2021

The

Язык: Английский

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Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Март 12, 2018

The brain adaptively integrates present sensory input, past experience, and options for future action. insect mushroom body exemplifies how a central structure brings about such integration. Here we use combination of systematic single-cell labeling, connectomics, transgenic silencing, activation experiments to study the at resolution, focusing on behavioral architecture its input output neurons (MBINs MBONs), intrinsic APL neuron. Our results reveal identity morphology almost all these 44 in stage 3 Drosophila larvae. Upon an initial screen, functional analyses medial lobe uncover sparse specific functions dopaminergic MBINs, MBONs, GABAergic neuron across three tasks, namely odor preference, taste associative learning between taste. thus provide cellular-resolution case brains organize behavior.

Язык: Английский

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The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor

Neuron, Год журнала: 2020, Номер 108(1), С. 145 - 163.e10

Опубликована: Сен. 10, 2020

Язык: Английский

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