Multilayered horizontal operon transfers from bacteria reconstruct a thiamine salvage pathway in yeasts DOI Open Access
Carla Gonçalves, Paula Gonçalves

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(44), С. 22219 - 22228

Опубликована: Окт. 14, 2019

Horizontal acquisition of bacterial genes is presently recognized as an important contribution to the adaptation and evolution eukaryotic genomes. However, mechanisms underlying expression consequent selection fixation prokaryotic in new setting are largely unknown. Here we show that composing pathway for synthesis essential vitamin B1 (thiamine) were lost ancestor a yeast lineage, Wickerhamiella/Starmerella (W/S) clade, known harbor unusually large number alien origin. The thiamine was subsequently reassembled, at least twice, by multiple HGT events from different donors involving both single entire operons. In W/S-clade species Starmerella bombicola obtained direct genetic evidence all functional. reconstructed composed operating coordinately scavenge derivatives environment. newly acquired operons involved repertoire until now only sparsely documented, namely longer intergenic regions, post-horizontal gene transfer (HGT) fusions fostering coordinated expression, relocation, possibly recombination generating mosaic genes. results provide additional occurred recurrently this lineage crucial reestablishment functions similar used across broad range microbes promote their

Язык: Английский

Comprehensive and Functional Analysis of Horizontal Gene Transfer Events in Diatoms DOI Open Access
Emmelien Vancaester, Thomas Depuydt, Cristina Maria Osuna‐Cruz

и другие.

Molecular Biology and Evolution, Год журнала: 2020, Номер 37(11), С. 3243 - 3257

Опубликована: Июль 10, 2020

Abstract Diatoms are a diverse group of mainly photosynthetic algae, responsible for 20% worldwide oxygen production, which can rapidly respond to favorable conditions and often outcompete other phytoplankton. We investigated the contribution horizontal gene transfer (HGT) its ecological success. A large-scale phylogeny-based prokaryotic HGT detection procedure across nine sequenced diatoms showed that 3–5% their proteome has origin large influx occurred at ancestor diatoms. More than 90% genes expressed, species-specific in Phaeodactylum tricornutum undergo strong purifying selection. Genes derived from implicated several processes including environmental sensing expand metabolic toolbox. Cobalamin (vitamin B12) is an essential cofactor roughly half only produced by bacteria. Five consecutive involved final synthesis cobalamin biosynthetic pathway, could function as scavenging repair genes, were detected HGT. The full suite these was cold-adapted diatom Fragilariopsis cylindrus. This might give originating Southern Ocean, region typically depleted cobalamin, competitive advantage. Overall, we show prevalent mechanism actively used adaptive capabilities.

Язык: Английский

Процитировано

47
A review of sophorolipid production from alternative feedstocks for the development of a localized selection strategy DOI
Phavit Wongsirichot, Benjamin Ingham, James Winterburn

и другие.

Journal of Cleaner Production, Год журнала: 2021, Номер 319, С. 128727 - 128727

Опубликована: Авг. 21, 2021

Язык: Английский

Процитировано

37
Ecological generalism drives hyperdiversity of secondary metabolite gene clusters in xylarialean endophytes DOI
Mario Emilio Ernesto Franco, Jennifer H. Wisecaver, A. Elizabeth Arnold

и другие.

New Phytologist, Год журнала: 2021, Номер 233(3), С. 1317 - 1330

Опубликована: Ноя. 19, 2021

Although secondary metabolites are typically associated with competitive or pathogenic interactions, the high bioactivity of endophytic fungi in Xylariales, coupled their abundance and broad host ranges spanning all lineages land plants lichens, suggests that enhanced metabolism might facilitate symbioses phylogenetically diverse hosts. Here, we examined metabolite gene clusters (SMGCs) across 96 Xylariales genomes two clades (Xylariaceae s.l. Hypoxylaceae), including 88 newly sequenced endophytes closely related saprotrophs pathogens. We paired genomic data extensive metadata on endophyte hosts substrates, enabling us to examine factors breadth symbiotic interactions ecological roles. All contain hyperabundant SMGCs; however, Xylariaceae have increased numbers duplications, horizontal transfers (HGTs) SMGCs. Enhanced metabolic diversity is a greater capacity for lignocellulose decomposition. Our results suggest that, as substrate generalists, experience selection diversify SMGCs compared more ecologically specialised Hypoxylaceae species. Overall, our provide new evidence may symbiosis hosts, highlighting importance microbial drive fungal diversity.

Язык: Английский

Процитировано

35
Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation DOI Open Access
Carlos A. Rosa, Marc‐André Lachance, Savitree Limtong

и другие.

Yeast, Год журнала: 2023, Номер 40(11), С. 511 - 539

Опубликована: Ноя. 1, 2023

Abstract Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central South America, Mexico, many Pacific Islands. These encompass less than 20% of Earth's terrestrial area, may contain about 50% the planet's biodiversity, endangered regions vulnerable to deforestation. have a great diversity substrates that can be colonized by yeasts. unicellular fungi contribute recycling organic matter, serve as food source for other organisms, or ecological interactions benefit harm plants, animals, fungi. In this review, we summarize most important studies yeast biodiversity carried out these biomes, well new data, discuss ecology genera frequently isolated from tropical forests potential microorganisms bioinnovation. We show forest represent tremendous species. Although studies, using culture‐dependent methods, already been Africa Australasia remain an underexplored novel hope review will encourage researchers study yeasts unexplored habitats.

Язык: Английский

Процитировано

14
Environment-dependent fitness gains can be driven by horizontal gene transfer of transporter-encoding genes DOI Creative Commons
David S. Milner, Victoria Attah, Emily Cook

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2019, Номер 116(12), С. 5613 - 5622

Опубликована: Март 6, 2019

Significance Horizontal gene transfer (HGT) is the of genetic information between genomes by a route other than from parent to offspring. Of particular interest here are transfers transporter-encoding genes, which can allow an organism utilize new metabolite, often via acquisition single foreign gene. Here we have identified range HGT events characterized substrate preferences for each encoded protein, and demonstrated that gain one these HGTs provide yeast with distinct competitive advantage in given environment. This has wide implications understanding how genes drastically influence environments fungi colonize.

Язык: Английский

Процитировано

41
Advances in the Study of Candida stellata DOI Creative Commons
Margarita Jiménez García, Braulio Esteve‐Zarzoso, Juan Mariano Cabellos

и другие.

Fermentation, Год журнала: 2018, Номер 4(3), С. 74 - 74

Опубликована: Сен. 4, 2018

Candida stellata is an imperfect yeast of the genus that belongs to order Saccharomycetales, while phylum Ascomycota. C. was isolated originally from a must overripe in Germany but widespread natural and artificial habitats. with taxonomic history characterized by numerous changes; it either heterogeneous species or easily confused other colonize same substrates. The strain DBVPG 3827, frequently used investigate oenological properties stellata, recently renamed as Starmerella bombicola, which can be zemplinina related like lactis-condensi. Strains have been processing foods feeds for thousands years. This species, commonly grape must, has found competitive persistent fermentation both white red wine various regions world tolerates concentration at least 9% (v/v) ethanol. Although these yeasts produce spoilage, several studies conducted characterize their ability desirable metabolites flavor, such acetate esters, presence enzymatic activities enhance aroma, β-glucosidase. microorganism could also possess many interesting technological applied food processing. Exo endoglucosidases polygalactosidase are important degradation β-glucans produced Botrytis cinerea. In traditional balsamic vinegar production, shapes aromatic profile vinegar, producing ethanol fructose high concentrations glycerol, succinic acid, ethyl acetate, acetoin. Chemical characterization exocellular polysaccharides non-Saccharomyces revealed them essentially mannoproteins mannose contents, ranging 73–74% bombicola. Numerous clearly proven macromolecules make multiple positive contributions quality. Recent on strains wines made co-fermentation Saccharomyces cerevisiae aroma attributes individual were apparent when inoculation protocol permitted growth activity yeasts. exploitation diversity biochemical sensory interest obtaining new products.

Язык: Английский

Процитировано

39
Growth and metabolite production of a grape sour rot yeast-bacterium consortium on different carbon sources DOI
Loris Pinto, Manuel Malfeito‐Ferreira, Laura Quintieri

и другие.

International Journal of Food Microbiology, Год журнала: 2019, Номер 296, С. 65 - 74

Опубликована: Март 1, 2019

Язык: Английский

Процитировано

39
The Wickerhamiella/Starmerella clade—A treasure trove for the study of the evolution of yeast metabolism DOI
Paula Gonçalves, Carla Gonçalves, Patrícia H. Brito

и другие.

Yeast, Год журнала: 2020, Номер 37(4), С. 313 - 320

Опубликована: Фев. 15, 2020

The Wickerhamiella and Starmerella genera form a clade (W/S clade) that branches close to Yarrowia lipolytica in the Saccharomycotina species tree. It comprises approximately 90 recognized 50 putative new not formally described yet. large majority of members W/S are ecologically associated with flowers floricolous insects. Many exhibit unusual metabolic traits, like fructophily production sophorolipids, which glycolipids can be used as environmentally friendly biosurfactants. Genomic data have only firmly established but also revealed tumultuous evolution metabolism marked by losses gains important pathways, among alcoholic fermentation. Possibly most surprising finding brought light comparative genomics concerned number genes acquired some from bacteria through horizontal gene transfer, many were shown functional their setting. This was facilitated genetic tractability one clade, bombicola, is for industrial sophorolipids. We suggest high-density coverage genome sequencing this combined possibility conduct molecular genetics experiments at least species, has potential set stage yet more exciting discoveries concerning yeast metabolism.

Язык: Английский

Процитировано

38
Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life DOI Creative Commons
Thomas LaBar, Yu-Ying Hsieh, Marco Fumasoni

и другие.

Current Biology, Год журнала: 2020, Номер 30(10), С. R565 - R574

Опубликована: Май 1, 2020

Язык: Английский

Процитировано

37
Multiple Reinventions of Mating-type Switching during Budding Yeast Evolution DOI Creative Commons

Tadeusz Krassowski,

Jacek Kominek, Xing‐Xing Shen

и другие.

Current Biology, Год журнала: 2019, Номер 29(15), С. 2555 - 2562.e8

Опубликована: Июль 25, 2019

Cell type in budding yeasts is determined by the genotype at mating-type (MAT) locus, but yeast species differ widely their mating compatibility systems and life cycles. Among sexual yeasts, heterothallic are those which haploid strains fall into two distinct stable types (MATa MATα), whereas homothallic that can switch or appear not to have [1Kurtzman C.P. Fell J.W. Boekhout T. The Yeasts, a Taxonomic Study. Elsevier, 2011Google Scholar, 2Wilson A.M. Wilken P.M. van der Nest M.A. Steenkamp E.T. Wingfield M.J. B.D. Homothallism: an umbrella term for describing diverse behaviours.IMA Fungus. 2015; 6: 207-214Crossref PubMed Scopus (58) Google Scholar]. evolutionary history of these uncertain, particularly regarding number direction transitions between homothallism heterothallism, whether process switching had single origin [3Lin X. Heitman J. Mechanisms fungi heterothallism homothallism.in: Kronstad Taylor Casselton L.A. Sex Fungi. ASM Press, 2007: 35-57Google 4Muller H. Hennequin C. Dujon B. Fairhead Ascomycetes: Candida MAT locus: comparing genomes hemiascomycetous yeasts.in: 247-263Google 5Hanson S.J. Wolfe K.H. An perspective on switching.Genetics. 2017; 206: 9-32Crossref (51) Here, we inferred 332 from genome sequences. By reference robust phylogenomic tree [6Shen X.X. Opulente D.A. Kominek Zhou Steenwyk J.L. Buh K.V. Haase M.A.B. Wisecaver J.H. Wang M. Doering D.T. et al.Tempo mode evolution subphylum.Cell. 2018; 175: 1533-1545Abstract Full Text PDF (225) Scholar], detected homothallism, among different forms homothallism. We find has arisen independently least 11 times during greatly outnumber opposite (31 versus 3). Although 3-locus MAT-HML-HMR mechanism as seen Saccharomyces cerevisiae simpler "flip/flop" mechanisms evolved separately 10 other groups yeasts. These results point adaptive value unicellular fungi.

Язык: Английский

Процитировано

36