Social state gates vision using three circuit mechanisms inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 17, 2024

Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent circuit motifs poised selectively amplify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioral neurophysiological analyses, show each these function during aggression. We reveal this same operate in males courtship pursuit, suggesting disparate behaviors may share mechanisms. Our work provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.

Язык: Английский

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A split-GAL4 driver line resource forDrosophilaneuron types

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

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A connectome of theDrosophilacentral complex reveals network motifs suitable for flexible navigation and context-dependent action selection

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2020, Номер unknown

Опубликована: Дек. 9, 2020

ABSTRACT Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron-microscopy-based connectome of Drosophila CX, including all its neurons circuits at synaptic resolution. identified new CX neuron types, novel sensory motor pathways, network motifs that likely extract fly’s head-direction, maintain it with attractor dynamics, combine other sensorimotor information perform vector-based navigational computations. also numerous pathways may facilitate selection CX-driven behavioral patterns by context internal state. The provides comprehensive blueprint necessary for detailed understanding underlying flexible navigation, state-dependent action selection.

Язык: Английский

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Neural mechanisms to exploit positional geometry for collision avoidance

Current Biology, Год журнала: 2022, Номер 32(11), С. 2357 - 2374.e6

Опубликована: Май 3, 2022

Язык: Английский

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Different spectral sensitivities of ON- and OFF-motion pathways enhance the detection of approaching color objects in Drosophila

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 24, 2023

Color and motion are used by many species to identify salient objects. They processed largely independently, but color contributes processing in humans, for example, enabling moving colored objects be detected when their luminance matches the background. Here, we demonstrate an unexpected, additional contribution of vision Drosophila. We show that behavioral ON-motion responses more sensitive UV than OFF-motion, cellular pathways connecting UV-sensitive R7 photoreceptors ON OFF-motion-sensitive T4 T5 cells, using neurogenetics calcium imaging. Remarkably, this circuitry enhances detection approaching discs, not green discs with same chromatic contrast, how could generalize systems ON- OFF-motion pathways. Our results provide a computational circuit basis favor saliently

Язык: Английский

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Shallow neural networks trained to detect collisions recover features of visual loom-selective neurons

eLife, Год журнала: 2022, Номер 11

Опубликована: Янв. 13, 2022

Animals have evolved sophisticated visual circuits to solve a vital inference problem: detecting whether or not signal corresponds an object on collision course. Such events are detected by specific sensitive looming, objects increasing in size. Various computational models been developed for these circuits, but how the collision-detection problem itself shapes structures of remains unknown. Here, inspired distinctive LPLC2 neurons system Drosophila, we build anatomically-constrained shallow neural network and train them identify signals that correspond impending collisions. Surprisingly, optimization arrives at two distinct, opposing solutions, only one which matches actual dendritic weighting neurons. Both solutions can with high accuracy when population size is large enough. The LPLC2-like reproduces experimentally observed neuron responses many stimuli, canonical tuning loom neurons, even though never trained data. Thus, properties predicted optimizing detect More generally, results illustrate tasks important animal's perceptual goals reveal explain sensory

Язык: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 21, 2022

Abstract Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. In order exploit this knowledge base, researchers target individual study function. Therefore, vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM). However, creating line for driving gene expression within single neuron found EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Here, we present NeuronBridge, web application easily rapidly putative morphological matches between datasets We describe functionality construction NeuronBridge service, including its user-friendly GUI, model, serverless cloud architecture, massively parallel image search engine. openly accessible at http://neuronbridge.janelia.org/ .

Язык: Английский

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Connectomic reconstruction predicts the functional organization of visual inputs to the navigation center of theDrosophilabrain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 30, 2023

Many animals, including humans, navigate their surroundings by visual input, yet we understand little about how information is transformed and integrated the navigation system. In

Язык: Английский

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Azimuthal invariance to looming stimuli in the Drosophila giant fiber escape circuit

Journal of Experimental Biology, Год журнала: 2023, Номер 226(8)

Опубликована: Апрель 15, 2023

ABSTRACT Spatially invariant feature detection is a property of many visual systems that rely on information provided by two eyes. However, how across both eyes integrated for not fully understood. Here, we investigated spatial invariance looming responses in descending neurons (DNs) Drosophila melanogaster. We found multiple responsive DNs integrate eyes, even though their dendrites are restricted to single hemisphere. One DN, the giant fiber (GF), responds invariantly stimuli tested azimuthal locations. confirmed propagates GF from contralateral eye, through an unidentified pathway, and demonstrated absence this pathway alters presented ipsilateral eye. Our data highlight role bilateral integration generating consistent, looming-evoked escape robust different stimulus locations parameters.

Язык: Английский

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eLife assessment: A split-GAL4 driver line resource for Drosophila CNS cell types

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Язык: Английский

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