A split-GAL4 driver line resource for Drosophila neuron types

Опубликована: Янв. 6, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

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Morphological and functional convergence of visual projection neurons from diverse neurogenic origins in Drosophila

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 15, 2025

The Drosophila visual system is a powerful model to study the development of neural circuits. Lobula columnar neurons-LCNs are output neurons that encode features relevant natural behavior. There ~20 classes LCNs forming non-overlapping synaptic optic glomeruli in brain. To address their origin, we used single-cell mRNA sequencing define transcriptome LCN subtypes and identified lines expressed throughout development. We show originate from stem cells four distinct brain regions exhibiting different modes neurogenesis, including ventral dorsal tips outer proliferation center, superficial inner center central this convergence similar illustrates complexity generating neuronal diversity, likely reflects evolutionary origin each subtype detects specific feature might influence behaviors species. Here authors developmental origins Columnar Neurons brain, tracing them regions. highlights diversity.

Язык: Английский

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A split-GAL4 driver line resource for Drosophila neuron types

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

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The Synaptic Complexity of a High‐Integration Lobula Giant Neuron in Crabs

The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(2)

Опубликована: Фев. 1, 2025

ABSTRACT Arthropods are diverse, abundant, successful animals that exploit all available ecological niches. They sense the environment, move, interact with prey/predators/conspecifics, learn, and so forth using small brains five orders of magnitude less neurons than mammals. Hence, these need to be efficient in information processing. One distinct aspect is presence large, easily identifiable single act as functional units for processing integrating a high volume from different sources guide behavior. To understand synaptic organization behind high‐integration nodes research on suitable needed. The lobula giant (LGs) found third optic neuropil, lobula, semiterrestrial crabs Neohelice granulata respond moving stimuli, integrate both eyes, show short‐ long‐term plasticity. thought key elements visuomotor transformation guiding escape responses approaching objects. subgroup, MLG1 (monostratified LG type 1), composed 16 have very wide main branches regular arrangement deep layer which allows their identification even unstained preparations. Here, we describe types abundance contacts involving profiles transmission electron microscopy (TEM). We an unexpected diversity motifs apparent compartmentalization dendritic arbor two domains where MLG1s predominantly presynaptic or postsynaptic, respectively. propose variety contact reflects multiple circuits cells involved. Regarding detection objects, distinctive input shared by locusts suggest similar collision‐detecting pathways species.

Язык: Английский

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Functional architecture of neural circuits for leg proprioception in Drosophila

Current Biology, Год журнала: 2021, Номер 31(23), С. 5163 - 5175.e7

Опубликована: Окт. 12, 2021

To effectively control their bodies, animals rely on feedback from proprioceptive mechanosensory neurons. In the Drosophila leg, different proprioceptor subtypes monitor joint position, movement direction, and vibration. Here, we investigate how these diverse sensory signals are integrated by central circuits. We find that for leg position directional converge in second-order neurons, revealing pathways local of posture. Distinct populations neurons integrate tibia vibration across pairs legs, suggesting a role detecting external substrate each pathway, flow information is dynamically gated sculpted inhibition. Overall, our results reveal parallel processing internal signals, which propose mediate sensing, respectively. The existence functional connectivity map also provides resource interpreting connectomic reconstruction neural circuits proprioception.

Язык: Английский

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