Distinctive features of the central synaptic organization of Drosophila larval proprioceptors DOI Creative Commons
Marie R. Greaney, Chris C Wreden, Ellie S. Heckscher

и другие.

Frontiers in Neural Circuits, Год журнала: 2023, Номер 17

Опубликована: Июль 26, 2023

Proprioceptive feedback is critically needed for locomotor control, but how this information incorporated into central proprioceptive processing circuits remains poorly understood. Circuit organization emerges from the spatial distribution of synaptic connections between neurons. This difficult to discern in model systems where only a few cells can be probed simultaneously. Therefore, we turned relatively simple and accessible nervous system ask: are proprioceptors’ input output synapses organized space, what principles underlie organization? Using Drosophila larval connectome, generated map 34 proprioceptors several adjacent body segments (5–6 left-right pairs per segment). We characterized these synapses, compared that other somatosensory neurons’ synapses. found three distinguishing features proprioceptor synapses: (1) Generally, individual types display segmental somatotopy. (2) Proprioceptor both converge diverge space; they six domains, each containing unique set one or more proprioceptors. Proprioceptors form along proximal axonal entry pathway neuropil. (3) receive inhibitory Further, find do not apply Thus, have most comprehensive date centrally organized. documents previously undescribed proprioceptors, raises questions about underlying developmental mechanisms, has implications downstream circuits.

Язык: Английский

Ascending neurons convey behavioral state to integrative sensory and action selection brain regions DOI Creative Commons
Chin‐Lin Chen, Florian Aymanns, Ryo Minegishi

и другие.

Nature Neuroscience, Год журнала: 2023, Номер 26(4), С. 682 - 695

Опубликована: Март 23, 2023

Knowing one's own behavioral state has long been theorized as critical for contextualizing dynamic sensory cues and identifying appropriate future behaviors. Ascending neurons (ANs) in the motor system that project to brain are well positioned provide such signals. However, what ANs encode where they convey these signals remains largely unknown. Here, through large-scale functional imaging behaving animals morphological quantification, we report encoding targeting of hundreds genetically identifiable adult fly, Drosophila melanogaster. We reveal states, specifically conveying self-motion anterior ventrolateral protocerebrum, an integrative hub, discrete actions gnathal ganglia, a locus action selection. Additionally, AN projection patterns within predictive their encoding. Thus, ascending populations poised inform distinct hubs ongoing behaviors may important substrate computations required adaptive behavior.

Язык: Английский

Процитировано

45
Connectomic reconstruction of a female Drosophila ventral nerve cord DOI
Anthony W. Azevedo, Ellen Lesser, Jasper S. Phelps

и другие.

Nature, Год журнала: 2024, Номер 631(8020), С. 360 - 368

Опубликована: Июнь 26, 2024

Язык: Английский

Процитировано

30
Systematic annotation of a complete adult maleDrosophilanerve cord connectome reveals principles of functional organisation DOI Creative Commons
Elizabeth C. Marin, Billy J Morris, Tomke Stürner

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 6, 2023

Summary Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Язык: Английский

Процитировано

29
Organization of an ascending circuit that conveys flight motor state in Drosophila DOI Creative Commons
Han SJ Cheong,

Kaitlyn Nicole Boone,

Marryn M. Bennett

и другие.

Current Biology, Год журнала: 2024, Номер 34(5), С. 1059 - 1075.e5

Опубликована: Фев. 22, 2024

Язык: Английский

Процитировано

15
Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation DOI Open Access
Elizabeth C. Marin, Billy J Morris, Tomke Stürner

и другие.

Опубликована: Июль 22, 2024

Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Язык: Английский

Процитировано

14
The processing of proprioceptive signals in distributed networks: insights from insect motor control DOI
Corinna Gebehart, Ansgar Büschges

Journal of Experimental Biology, Год журнала: 2024, Номер 227(1)

Опубликована: Янв. 1, 2024

ABSTRACT The integration of sensory information is required to maintain body posture and generate robust yet flexible locomotion through unpredictable environments. To anticipate adaptations in limb enable compensation sudden perturbations, an animal's nervous system assembles external (exteroception) internal (proprioception) cues. Coherent neuronal representations the proprioceptive context appendages arise from concerted action multiple sense organs monitoring kinetics kinematics. This multimodal information, together with exteroceptive signals brain-derived descending motor commands, converges onto premotor networks – i.e. local circuitry controlling output movements within ventral nerve cord (VNC), insect equivalent vertebrate spinal cord. Review summarizes existing knowledge recent advances understanding how VNC use convergent contextually appropriate activity, focusing on example control. We compare role advantages distributed processing over dedicated pathways, challenges networks. discuss gain may be tuned behavioral repertoire these systems, argue that might compensate for their limited population size by, comparison networks, relying more heavily specificity connections. At a time which connectomics physiological recording techniques anatomical functional circuit dissection at unprecedented resolution, systems offer unique opportunities identify mechanisms underlying

Язык: Английский

Процитировано

9
Miniature linear and split-belt treadmills reveal mechanisms of adaptive motor control in walking Drosophila DOI
Brandon Pratt, Su-Yee J. Lee, Grant M Chou

и другие.

Current Biology, Год журнала: 2024, Номер 34(19), С. 4368 - 4381.e5

Опубликована: Авг. 30, 2024

Язык: Английский

Процитировано

6
Tools for connectomic reconstruction and analysis of a femaleDrosophilaventral nerve cord DOI Creative Commons
Anthony W. Azevedo, Ellen Lesser, Jasper S. Phelps

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 15, 2022

Summary Like the vertebrate spinal cord, insect ventral nerve cord (VNC) mediates limb sensation and motor control. Here, we apply automated tools for electron microscopy volume alignment, neuron segmentation, synapse prediction toward creating a connectome of an adult female Drosophila VNC. To interpret connectome, it is crucial to know its relationship with rest body. We therefore mapped muscle targets leg wing neurons in by comparing their morphology genetic driver lines, dye fills, X-ray nano-tomography fly wing. Knowing outputs allowed us identify neural circuits that coordinate wings legs during escape takeoff. provide reconstruction VNC atlas, along programmatic interactive access, as community resources support experimental theoretical studies how nervous system controls behavior.

Язык: Английский

Процитировано

26
Biomechanical origins of proprioceptor feature selectivity and topographic maps in the Drosophila leg DOI Creative Commons
Akira Mamiya, Anne Sustar, Igor Siwanowicz

и другие.

Neuron, Год журнала: 2023, Номер 111(20), С. 3230 - 3243.e14

Опубликована: Авг. 9, 2023

Язык: Английский

Процитировано

15
Presynaptic inhibition selectively suppresses leg proprioception in behavingDrosophila DOI Creative Commons
Chris J. Dallmann, Yichen Luo, Sweta Agrawal

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 23, 2023

Controlling arms and legs requires feedback from proprioceptive sensory neurons that detect joint position movement. Proprioceptive must be tuned for different behavioral contexts, but the underlying circuit mechanisms remain poorly understood. Using calcium imaging in behaving

Язык: Английский

Процитировано

11