A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Год журнала: 2021, Номер 10

Опубликована: Окт. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Язык: Английский

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A neural circuit for wind-guided olfactory navigation

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Авг. 8, 2022

Abstract To navigate towards a food source, animals frequently combine odor cues about source identity with wind direction location. Where and how these two are integrated to support navigation is unclear. Here we describe pathway the Drosophila fan-shaped body that encodes attractive promotes upwind navigation. We show neurons throughout this encode odor, but not direction. Using connectomics, identify local called h∆C receive input from previously described pathway. exhibit odor-gated, direction-tuned activity, sparse activation of in reproducible direction, activity required for persistent orientation during odor. Based on connectome data, develop computational model showing can promote goal such as an source. Our results suggest processed by separate pathways within goal-directed

Язык: Английский

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Connectomics and the neural basis of behaviour

Current Opinion in Insect Science, Год журнала: 2022, Номер 54, С. 100968 - 100968

Опубликована: Сен. 13, 2022

Methods to acquire and process synaptic-resolution electron-microscopy datasets have progressed very rapidly, allowing production annotation of larger, more complete connectomes. More accurate neuronal matching techniques are enriching cell type data with gene expression, neuron activity, behaviour developmental information, providing ways test hypotheses circuit function. In a variety behaviours such as learned innate olfaction, navigation sexual behaviour, connectomics has already revealed interconnected modules hierarchical structure, recurrence integration sensory streams. Comparing individual connectomes determine which features robust variable is one key research area; new work in comparative across development, experience, sex species will establish strong links between connectivity brain

Язык: Английский

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Neural Circuits Underlying Behavioral Flexibility: Insights From Drosophila

Frontiers in Behavioral Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Янв. 6, 2022

Behavioral flexibility is critical to survival. Animals must adapt their behavioral responses based on changes in the environmental context, internal state, or experience. Studies Drosophila melanogaster have provided insight into neural circuit mechanisms underlying flexibility. Here we discuss how behavior modulated by and learning. We describe general principles of organization modulation that underlie flexibility, are likely extend other species.

Язык: Английский

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Visual processing in the fly, from photoreceptors to behavior

Genetics, Год журнала: 2023, Номер 224(2)

Опубликована: Май 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Язык: Английский

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Neural circuit mechanisms for transforming learned olfactory valences into wind-oriented movement

eLife, Год журнала: 2023, Номер 12

Опубликована: Сен. 18, 2023

How memories are used by the brain to guide future action is poorly understood. In olfactory associative learning in Drosophila, multiple compartments of mushroom body act parallel assign a valence stimulus. Here, we show that appetitive stored different induce levels upwind locomotion. Using photoactivation screen new collection split-GAL4 drivers and EM connectomics, identified cluster neurons postsynaptic output (MBONs) can trigger robust steering. These UpWind Neurons (UpWiNs) integrate inhibitory excitatory synaptic inputs from MBONs aversive memory compartments, respectively. After formation memory, UpWiNs acquire enhanced response reward-predicting odors as presynaptic MBON undergoes depression. Blocking impaired reduced locomotion during retrieval. Photoactivation also increased chance returning location where activation was terminated, suggesting an additional role navigation. Thus, our results provide insight into how learned abstract valences gradually transformed concrete memory-driven actions through divergent convergent networks, neuronal architecture commonly found vertebrate invertebrate brains.

Язык: Английский

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Olfactory navigation in arthropods

Journal of Comparative Physiology A, Год журнала: 2023, Номер 209(4), С. 467 - 488

Опубликована: Янв. 20, 2023

Abstract Using odors to find food and mates is one of the most ancient highly conserved behaviors. Arthropods from flies moths crabs use broadly similar strategies navigate toward odor sources—such as integrating flow information with information, comparing concentration across sensors, over time. Because arthropods share many homologous brain structures—antennal lobes for processing olfactory mechanosensors flow, mushroom bodies (or hemi-ellipsoid bodies) associative learning, central complexes navigation, it likely that these closely related behaviors are mediated by neural circuits. However, differences in types they seek, physics dispersal, locomotion water, air, on substrates mean circuits must have adapted generate a wide diversity odor-seeking In this review, we discuss common specializations observed navigation behavior arthropods, review our current knowledge about subserving behavior. We propose comparative study arthropod nervous systems may provide insight into how set basic circuit structures has diversified different environments.

Язык: Английский

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Wind gates olfaction-driven search states in free flight

Current Biology, Год журнала: 2024, Номер 34(19), С. 4397 - 4411.e6

Опубликована: Июль 26, 2024

Язык: Английский

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Driver lines for studying associative learning in Drosophila

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 29, 2025

The mushroom body (MB) is the center for associative learning in insects. In Drosophila , intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending olfactory thermo-/hygro-sensory projection interneurons connected with MB-extrinsic various other types. We characterized activation phenotypes subset these lines identified neuron line most suitable reward substitution. Leveraging thousands confocal images associated collection, analyzed neuronal morphological stereotypy discovered one set output MBON08/MBON09, exhibits striking individuality asymmetry across animals. conjunction EM connectome maps, reported here offer powerful resource functional dissection circuits adult .

Язык: Английский

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Transsynaptic mapping of Drosophila mushroom body output neurons

eLife, Год журнала: 2021, Номер 10

Опубликована: Фев. 10, 2021

The mushroom body (MB) is a well-characterized associative memory structure within the

Язык: Английский

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