Connectome-driven neural inventory of a complete visual system

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 18, 2024

Vision provides animals with detailed information about their surroundings, conveying diverse features such as color, form, and movement across the visual scene. Computing these parallel spatial requires a large network of neurons, that in distant flies humans, regions comprise half brain's volume. These brain often reveal remarkable structure-function relationships, neurons organized along maps shapes directly relate to roles processing. To unravel stunning diversity complex system, careful mapping neural architecture matched tools for targeted exploration circuitry is essential. Here, we report new connectome right optic lobe from male Drosophila central nervous system FIB-SEM volume comprehensive inventory fly's neurons. We developed computational framework quantify anatomy establishing basis interpreting how vision. By integrating this analysis connectivity information, neurotransmitter identity, expert curation, classified ~53,000 into 727 types, which are systematically described named first time. Finally, share an extensive collection split-GAL4 lines our neuron type catalog. Together, set data unlock possibilities systematic investigations vision Drosophila, foundation deeper understanding sensory

Язык: Английский

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Connectome-constrained networks predict neural activity across the fly visual system

Nature, Год журнала: 2024, Номер 634(8036), С. 1132 - 1140

Опубликована: Сен. 11, 2024

Язык: Английский

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Neuronal parts list and wiring diagram for a visual system

Nature, Год журнала: 2024, Номер 634(8032), С. 166 - 180

Опубликована: Окт. 2, 2024

Язык: Английский

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Connectome-driven neural inventory of a complete visual system

Nature, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Abstract Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form movement across the visual scene. Computing these parallel spatial requires a large network of neurons. Consequently, from flies to humans, regions in brain constitute half its volume. These often have marked structure–function relationships, neurons organized along maps shapes that directly relate roles processing. More than century anatomical studies catalogued detail cell types fly systems 1–3 , behavioural physiological experiments examined capabilities flies. To unravel diversity complex system, careful mapping neural architecture matched tools for targeted exploration this circuitry is essential. Here we present connectome right optic lobe male Drosophila melanogaster acquired using focused ion beam milling scanning electron microscopy. We established comprehensive inventory developed computational framework quantify anatomy. Together, data establish basis interpreting how vision. By integrating analysis connectivity information, neurotransmitter identity expert curation, classified approximately 53,000 into 732 types. are systematically described newly named. Finally, share an extensive collection split-GAL4 lines our neuron-type catalogue. Overall, set unlocks new possibilities systematic investigations vision foundation deeper understanding sensory

Язык: Английский

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Small-field visual projection neurons detect translational optic flow and support walking control

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 22, 2023

SUMMARY Animals rely on visual motion for navigating the world, and research in flies has clarified how neural circuits extract information from moving scenes. However, major pathways connecting these patterns of optic flow to behavior remain poorly understood. Using a high-throughput quantitative assay visually guided behaviors genetic neuronal silencing, we discovered region Drosophila ’s protocerebrum critical following. We used calcium imaging, optogenetics identify single cell type, LPC1, that innervates this region, detects translational flow, plays key role regulating forward walking. Moreover, population LPC1s can estimate travelling direction, such as when gaze direction diverges body heading. By linking specific types their computations behaviors, our findings establish foundation understanding nervous system uses vision guide navigation.

Язык: Английский

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Social state gates vision using three circuit mechanisms inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 17, 2024

Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well-studied. Yet, much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviors, flies need to focus nearby flies. Here, we study the flow of altered when female Drosophila enter aggressive state. From connectome, identified three state-dependent circuit motifs poised selectively amplify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioral neurophysiological analyses, show each these function during aggression. We reveal this same operate in males courtship pursuit, suggesting disparate behaviors may share mechanisms. Our work provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.

Язык: Английский

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Mapping model units to visual neurons reveals population code for social behaviour

Nature, Год журнала: 2024, Номер 629(8014), С. 1100 - 1108

Опубликована: Май 22, 2024

Abstract The rich variety of behaviours observed in animals arises through the interplay between sensory processing and motor control. To understand these sensorimotor transformations, it is useful to build models that predict not only neural responses input 1–5 but also how each neuron causally contributes behaviour 6,7 . Here we demonstrate a novel modelling approach identify one-to-one mapping internal units deep network real neurons by predicting behavioural changes arise from systematic perturbations more than dozen neuronal cell types. A key ingredient introduce ‘knockout training’, which involves perturbing during training match experiments. We apply this model transformations Drosophila melanogaster males complex, visually guided social 8–11 visual projection at interface optic lobe central brain form set discrete channels 12 , prior work indicates channel encodes specific feature drive particular 13,14 Our reaches different conclusion: combinations neurons, including those involved non-social behaviours, male interactions with female, forming population code for behaviour. Overall, our framework consolidates effects elicited various into single, unified model, providing map stimulus type behaviour, enabling future incorporation wiring diagrams 15 model.

Язык: Английский

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Predicting visual function by interpreting a neuronal wiring diagram

Nature, Год журнала: 2024, Номер 634(8032), С. 113 - 123

Опубликована: Окт. 2, 2024

Язык: Английский

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Sensory quiescence induces a cell-non-autonomous integrated stress response curbed by condensate formation of the ATF4 and XRP1 effectors

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Sensory disabilities have been identified as significant risk factors for dementia but underlying molecular mechanisms are unknown. In different Drosophila models with loss of sensory input, we observe non-autonomous induction the integrated stress response (ISR) deep in brain, indicated by eIF2αS50 phosphorylation-dependent elevated levels ISR effectors ATF4 and XRP1. Unlike during canonical ISR, however, XRP1 transcription enriched cytosolic granules that positive RNA granule markers Caprin, FMR1, p62, reversible upon restoration vision blind flies. Cytosolic restraint dampens expression their downstream targets including genes cell death pathways activated chronic cellular thus constitutes a protective (CSPR). containing both p62 also evident thalamus hippocampus mouse congenital or degenerative blindness. These data indicate conserved link between input curbed responses critical protein quality control brain. Chronic can be damaging hazards they counteract. Here authors show how blindness-induced brain-wide is dampened sequestration granules.

Язык: Английский

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Temporal and Notch identity determine layer targeting and synapse location of medulla neurons

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

How specification mechanisms that generate neural diversity translate into specific neuronal targeting, connectivity, and function in the adult brain is not understood. In medulla region of Drosophila optic lobe, progenitors different neurons a fixed order by sequentially expressing series temporal transcription factors as they age. Then, Notch signaling intermediate further diversifies progeny. By establishing birth neurons, we found their identity correlates with depth neuropil targeting brain, for both local interneurons projection neurons. We show this identity-dependent unfolds early development genetically determined. leveraging Electron Microscopy reconstruction fly determined synapse location lobe neuropils find it significantly associated status. Moreover, all putative same predicted share similar location, indicating ensembles layers encode visual functions. conclusion, status can predict function, linking developmental patterning connectivity functional features brain.

Язык: Английский

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