Large-scale neural recordings call for new insights to link brain and behavior

Nature Neuroscience, Год журнала: 2022, Номер 25(1), С. 11 - 19

Опубликована: Янв. 1, 2022

Язык: Английский

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Facemap: a framework for modeling neural activity based on orofacial tracking

Nature Neuroscience, Год журнала: 2023, Номер 27(1), С. 187 - 195

Опубликована: Ноя. 20, 2023

Recent studies in mice have shown that orofacial behaviors drive a large fraction of neural activity across the brain. To understand nature and function these signals, we need better computational models to characterize relate them activity. Here developed Facemap, framework consisting keypoint tracker deep network encoder for predicting Our algorithm tracking mouse was more accurate than existing pose estimation tools, while processing speed several times faster, making it powerful tool real-time experimental interventions. The Facemap easy adapt data from new labs, requiring as few 10 annotated frames near-optimal performance. We used keypoints inputs which predicts ~50,000 simultaneously-recorded neurons and, visual cortex, doubled amount explained variance compared previous methods. Using this model, found neuronal clusters were well predicted behavior spatially spread out cortex. also behavioral features model had stereotypical, sequential dynamics not reversible time. In summary, provides stepping stone toward understanding brain-wide signals their relation behavior.

Язык: Английский

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A Brain-Wide Map of Neural Activity during Complex Behaviour

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 4, 2023

Abstract A key challenge in neuroscience is understanding how neurons hundreds of interconnected brain regions integrate sensory inputs with prior expectations to initiate movements. It has proven difficult meet this when different laboratories apply analyses recordings during behaviours. Here, we report a comprehensive set from 115 mice 11 labs performing decision-making task sensory, motor, and cognitive components, obtained 547 Neuropixels probe insertions covering 267 areas the left forebrain midbrain right hindbrain cerebellum. We provide an initial appraisal brain-wide map, assessing neural activity encodes variables. Representations visual stimuli appeared transiently classical after stimulus onset then spread ramp-like collection mid- that also encoded choices. Neural responses correlated motor action almost everywhere brain. Responses reward delivery consumption versus omission were widespread. objective weaker, found sparse sets restricted regions. This publicly available dataset represents unprecedented resource for computations distributed across within drive behaviour.

Язык: Английский

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Low-threshold, high-resolution, chronically stable intracortical microstimulation by ultraflexible electrodes

Cell Reports, Год журнала: 2023, Номер 42(6), С. 112554 - 112554

Опубликована: Май 24, 2023

Intracortical microstimulation (ICMS) enables applications ranging from neuroprosthetics to causal circuit manipulations. However, the resolution, efficacy, and chronic stability of neuromodulation are often compromised by adverse tissue responses indwelling electrodes. Here we engineer ultraflexible stim-nanoelectronic threads (StimNETs) demonstrate low activation threshold, high chronically stable ICMS in awake, behaving mouse models. In vivo two-photon imaging reveals that StimNETs remain seamlessly integrated with nervous throughout stimulation periods elicit stable, focal neuronal at currents 2 μA. Importantly, evoke longitudinally behavioral for over 8 months a markedly charge injection 0.25 nC/phase. Quantified histological analyses show induces no degeneration or glial scarring. These results suggest tissue-integrated electrodes provide path robust, long-lasting, spatially selective currents, which lessens risk damage exacerbation off-target side effects.

Язык: Английский

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Trial-history biases in evidence accumulation can give rise to apparent lapses in decision-making

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 22, 2024

Abstract Trial history biases and lapses are two of the most common suboptimalities observed during perceptual decision-making. These routinely assumed to arise from distinct processes. However, previous work has suggested that they covary in their prevalence proposed neural substrates overlap. Here we demonstrate decision-making, apparent can both a cognitive process is optimal under mistaken beliefs world changing i.e. nonstationary. This corresponds an accumulation-to-bound model with history-dependent updates initial state accumulator. We test our model’s predictions about relative lapses, show robustly borne out decision-making datasets male rats, including data novel reaction time task. Our improves ability precisely predict dynamics within across trials, by positing through which agents generate quasi-stochastic choices.

Язык: Английский

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Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations, and especially powerful when combined with optogenetics manipulation of specific cell types high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic photonic circuits. These devices pack 960 electrical sites two sets 14 light emitters onto a 1 cm shank, allowing spatially addressable optogenetic stimulation blue red light. In mouse cortex, probes delivered high-quality recordings together optogenetics, differentially activating or silencing neurons at distinct cortical depths. striatum other deep structures, efficient optotagging, facilitating identification in parallel. represent an unprecedented tool recording, identifying, manipulating neuronal populations.

Язык: Английский

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Neocortex saves energy by reducing coding precision during food scarcity

Neuron, Год журнала: 2021, Номер 110(2), С. 280 - 296.e10

Опубликована: Ноя. 5, 2021

Information processing is energetically expensive. In the mammalian brain, it unclear how information coding and energy use are regulated during food scarcity. Using whole-cell recordings two-photon imaging in layer 2/3 mouse visual cortex, we found that restriction reduced AMPA receptor conductance, reducing synaptic ATP by 29%. Neuronal excitability was nonetheless preserved a compensatory increase input resistance depolarized resting potential. Consequently, neurons spiked at similar rates as controls but spent less on underlying excitatory currents. This energy-saving strategy had cost because amplified variability of visually-evoked subthreshold responses, leading to 32% broadening orientation tuning impaired fine discrimination. reduction precision associated with levels fat mass-regulated hormone leptin restored exogenous supplementation. Our findings reveal metabolic state dynamically regulates neocortex.

Язык: Английский

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Functional neuroimaging in psychiatry and the case for failing better

Neuron, Год журнала: 2022, Номер 110(16), С. 2524 - 2544

Опубликована: Авг. 1, 2022

Язык: Английский

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Open-source, Python-based, hardware and software for controlling behavioural neuroscience experiments

eLife, Год журнала: 2022, Номер 11

Опубликована: Янв. 19, 2022

Laboratory behavioural tasks are an essential research tool. As questions asked of behaviour and brain activity become more sophisticated, the ability to specify run richly structured becomes important. An increasing focus on reproducibility also necessitates accurate communication task logic other researchers. To these ends, we developed pyControl, a system open-source hardware software for controlling experiments comprising simple yet flexible Python-based syntax specifying as extended state machines, modules building setups, graphical user interface designed efficiently running high-throughput many setups in parallel, all with extensive online documentation. These tools make it quicker, easier, cheaper implement rich at scale. important, pyControl facilitates through highly readable definition self-documenting features. Here, outline system's design rationale, present validation characterising performance, demonstrate example applications freely moving head-fixed mouse behaviour.

Язык: Английский

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Neural correlates of blood flow measured by ultrasound

Neuron, Год журнала: 2022, Номер 110(10), С. 1631 - 1640.e4

Опубликована: Март 11, 2022

Functional ultrasound imaging (fUSI) is an appealing method for measuring blood flow and thus infer brain activity, but it relies on the physiology of neurovascular coupling requires extensive signal processing. To establish to what degree fUSI trial-by-trial signals reflect neural we performed simultaneous recordings with Neuropixels probes in awake mice. strongly correlated slow (<0.3 Hz) fluctuations local firing rate were closely predicted by smoothed neurons, particularly putative inhibitory neurons. The optimal smoothing filter had a width ∼3 s, matched hemodynamic response function mice, was invariant across mice stimulus conditions, similar cortex hippocampus. also spatially: rates as highly hemispheres signals. Thus, measured bears simple accurate relationship neuronal firing.

Язык: Английский

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