Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis DOI Creative Commons
Yiming Chao,

Yang Xiang,

Jiashun Xiao

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Сен. 3, 2022

Abstract Single-cell level characterization of embryonic development is a major benchmark human developmental biology. Spatiotemporal analysis stem-cell-derived embryos offers conceptual and technical advances in the field. Here, we defined single-cell spatiotemporal gene expression landscape with organoids. We established organoid (HEMO) from expanded potential stem cells achieved both extraembryonic tissues same organoid. Time-series RNA sequencing paired resolution spatial revealed signatures such as placenta, yolk sac hematopoiesis neural crest, blood vessels, cardiac mesoderm. Hematopoietic eventually predominated HEMO erythropoiesis, mekagaryopiesis, myelopoiesis. Cell-cell communication network demonstrated that trophoblast-like supplied WNT signaling crest to facilitate maturation migration. transcriptomics erythro-megakaryopoietic niche. Vitronectin-integrin signaling, contributor megakaryocyte maturation, was predominant niche fetal samples. Overall, our study Highlights Modeling Used 10X Chromium Visium define regulator EMT VTN-ITGA2B main Mk within Graphical abstract

Язык: Английский

3D gastruloids: a novel frontier in stem cell-based in vitro modeling of mammalian gastrulation DOI Creative Commons
Susanne C. van den Brink, Alexander van Oudenaarden

Trends in Cell Biology, Год журнала: 2021, Номер 31(9), С. 747 - 759

Опубликована: Июль 22, 2021

3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects gastrula-stage embryos, have emerged as a powerful tool to study the early stages mammalian post-implantation development in vitro. Owing their tractable nature and relative ease by which they can be generated large numbers, gastruloids provide an unparalleled opportunity normal pathological embryogenesis from bottom-up perspective high-throughput manner. Here, we review how gastruloid models exploited deepen our understanding development. In addition, discuss current limitations, potential clinical applications, ethical implications this emerging model system.

Язык: Английский

Процитировано

90
Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking DOI
Harold M. McNamara, Sabrina C. Solley, Britt Adamson

и другие.

Nature Cell Biology, Год журнала: 2024, Номер 26(11), С. 1832 - 1844

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

11
Sculpting with stem cells: how models of embryo development take shape DOI Creative Commons
Jesse V. Veenvliet, Pierre‐François Lenne, David A. Turner

и другие.

Development, Год журнала: 2021, Номер 148(24)

Опубликована: Дек. 15, 2021

ABSTRACT During embryogenesis, organisms acquire their shape given boundary conditions that impose geometrical, mechanical and biochemical constraints. A detailed integrative understanding how these morphogenetic information modules pattern the mammalian embryo is still lacking, mostly owing to inaccessibility of in vivo for direct observation manipulation. These impediments are circumvented by developmental engineering embryo-like structures (stembryos) from pluripotent stem cells easy access, track, manipulate scale. Here, we explain unlocking distinct levels architecture through controlled modulations cellular environment enables identification minimal sets inputs necessary embryo. We detail this can be complemented with precise measurements manipulations tissue biochemistry, mechanics geometry across spatial temporal scales provide insights into mechanochemical feedback loops governing morphogenesis. Finally, discuss how, even absence active manipulations, stembryos display intrinsic phenotypic variability leveraged define constraints ensure reproducible morphogenesis vivo.

Язык: Английский

Процитировано

46
The evolution of gastrulation morphologies DOI Creative Commons
Guillermo Serrano Nájera, Cornelis J. Weijer

Development, Год журнала: 2023, Номер 150(7)

Опубликована: Апрель 1, 2023

During gastrulation, early embryos specify and reorganise the topology of their germ layers. Surprisingly, this fundamental process does not appear to be rigidly constrained by evolutionary pressures; instead, morphology gastrulation is highly variable throughout animal kingdom. Recent experimental results demonstrate that it possible generate different alternative modes in single organisms, such as cnidarian, arthropod vertebrate embryos. Here, we review mechanisms underlie plasticity both when experimentally manipulated during evolution. Using insights obtained from these experiments discuss effects increase yolk volume on provide new into two crucial innovations amniote gastrulation: transition a ring-shaped mesoderm domain anamniotes crescent-shaped amniotes, evolution reptilian blastoporal plate/canal avian primitive streak.

Язык: Английский

Процитировано

19
Early autonomous patterning of the anteroposterior axis in gastruloids DOI Creative Commons
Kerim Anlaş, Nicola Gritti, Fumio Nakaki

и другие.

Development, Год журнала: 2024, Номер 151(22)

Опубликована: Ноя. 15, 2024

Minimal in vitro systems composed of embryonic stem cells (ESCs) have been shown to recapitulate the establishment anteroposterior (AP) axis. In contrast native embryo, ESC aggregates - such as gastruloids can break symmetry, which is demarcated by polarization mesodermal marker T, autonomously without any localized external cues. However, associated earliest patterning events, spatial restriction cell fates and concomitant transcriptional changes, remain poorly understood. Here, we dissect dynamics AP axis mouse gastruloids, particularly before Wnt stimulation. Through single-cell RNA sequencing, identify key state transitions molecular signatures T+ T- populations underpinning polarization. We also show that this process robust modifications aggregate size. Finally, transcriptomic comparison with embryo indicates develop similar mesendodermal types, despite initial differences their primed pluripotent populations, adopt a more mesenchymal lieu an epiblast-like transcriptome. Hence, our findings suggest possibility alternate states vivo converge onto fates.

Язык: Английский

Процитировано

6
Carnegie in 4D? Stem-cell-based models of human embryo development DOI
Berna Sözen,

Deniz Conkar,

Jesse V. Veenvliet

и другие.

Seminars in Cell and Developmental Biology, Год журнала: 2022, Номер 131, С. 44 - 57

Опубликована: Июнь 11, 2022

Язык: Английский

Процитировано

20
Coordination between endoderm progression and mouse gastruloid elongation controls endodermal morphotype choice DOI

Naama Farag,

Chen Sacharen,

Lara Avni

и другие.

Developmental Cell, Год журнала: 2024, Номер 59(17), С. 2364 - 2374.e4

Опубликована: Июнь 5, 2024

Язык: Английский

Процитировано

5
Versatile system cores as a conceptual basis for generality in cell and developmental biology DOI Creative Commons
Elisa Cristina De Proença Rodrigues Gallo, Stefano De Renzis, James Sharpe

и другие.

Cell Systems, Год журнала: 2024, Номер 15(9), С. 790 - 807

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

5
MUM, a maternal unknown message, inhibits early establishment of the medio-lateral axis in the embryo of the kelpSaccharina latissima DOI Creative Commons
Samuel Boscq, Bernard Billoud, Ioannis Théodorou

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 8, 2024

Abstract Brown algae are multicellular photosynthetic organisms that have evolved independently of plants and other algae. Apart from studies on the polarisation Fucus zygote in 1990s, little is known about mechanisms controlling embryogenesis these organisms. Here, we studied determinism kelp Saccharina latissima, focusing formation its body axes. This alga initially develops an oblong embryo formed a cell monolayer, which subsequently thickens; growth axes then established temporally distinct stages, starting with dominant apico-basal axis. Our experiments focused role stalk, empty retains attached to maternal tissue, development mature embryos. In response removal embryos developed as monolayered disc rather than elongated blade, demonstrating attachment stalk inhibits medio-lateral axis at onset embryogenesis. addition, compared cells detached were smaller displayed altered shape. The topology tissue was also disrupted, had higher number neighbours. Observation division patterns just after showed represses longitudinal divisions, thereby reinforcing establishment main unique quantitative study brown algal revealed that, kelps, signal (MUM for unknown message) necessary organisation growing embryonic tissues. discovered although persists several weeks until reaches least 500 cells, MUM emitted first 4 days fertilisation only, before 8-cell stage. Finally, transplantation indicated does not diffuse seawater, but requires contact between stalk. potential chemical or mechanical nature discussed.

Язык: Английский

Процитировано

4
The logic of monsters: development and morphological diversity in stem-cell-based embryo models DOI Creative Commons
Dominica Cao, Sumit Garai, James DiFrisco

и другие.

Interface Focus, Год журнала: 2024, Номер 14(5)

Опубликована: Окт. 25, 2024

Organoids and stem-cell-based embryo models (SEMs) are imperfect organ or representations that explore a much larger space of possible forms, morphospace, compared to their

Язык: Английский

Процитировано

3