Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(2)

Опубликована: Фев. 1, 2024

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.

Язык: Английский

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Small extracellular vesicles isolation and separation: Current techniques, pending questions and clinical applications

Theranostics, Год журнала: 2022, Номер 12(15), С. 6548 - 6575

Опубликована: Янв. 1, 2022

Extracellular vesicles, especially small extracellular vesicles (sEVs) are now accepted as important messengers in cell-to-cell communication and a promising drug delivery platform.They involved nearly all physiological pathological processes disease diagnosis therapy.However, their heterogeneity of physicochemical properties functions is not fully understood, which hinders further clinical applications.To obtain highly bioactive sEVs with both high yield purity, will certainly facilitate future study application.This review informs up-to-date research on frequently-used cutting-edge technologies isolation makes deep comparison analysis different methods, including advantages, limitations applications.Pending questions about the inherent property these well strategies discussed.Additionally, an overview applications treatment, some on-going trials, also reviewed.

Язык: Английский

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Recent developments in isolating methods for exosomes

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 10

Опубликована: Янв. 13, 2023

Exosomes are the smallest extracellular vesicles that can be released by practically all cell types, and range in size from 30 nm to 150 nm. As major marker of liquid biopsies, exosomes have great potential for disease diagnosis, therapy, prognosis. However, their inherent heterogeneity, complexity biological fluids, presence nanoscale contaminants make isolation a challenge. Traditional methods cumbersome challenging with complex time-consuming operations. In recent years, emergence microfluidic chips, nanolithography, electro-deposition, other technologies has promoted combination innovation methods. The application these brought very considerable benefits such as ultra-fast, portable integration, low loss. There significant functional improvements yield, purity, clinical applications. this review, series summarized, emphasis on emerging methods, in-depth comparison analysis each method provided, including principles, merits, demerits.

Язык: Английский

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Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles

ACS Nano, Год журнала: 2022, Номер 16(8), С. 11619 - 11645

Опубликована: Июль 29, 2022

Extracellular vesicles (EVs) are complex lipid membrane vehicles with variable expressions of molecular cargo, composed diverse subpopulations that participate in the intercellular signaling biological responses disease. EV-based liquid biopsies demonstrate invaluable clinical potential for overhauling current practices disease management. Yet, EV heterogeneity is a major needle-in-a-haystack challenge to translate their use into practice. In this review, existing digital assays will be discussed analyze EVs at single vesicle resolution, and future opportunities optimize throughput, multiplexing, sensitivity highlighted. Furthermore, review outline challenges impact translation technologies diagnostics treatment monitoring.

Язык: Английский

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A review on comparative studies addressing exosome isolation methods from body fluids

Analytical and Bioanalytical Chemistry, Год журнала: 2022, Номер 415(7), С. 1239 - 1263

Опубликована: Июль 15, 2022

Язык: Английский

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Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 4, 2023

Exosomes and extracellular vesicles (EV) are increasingly being explored as circulating biomarkers, but their heterogenous composition will likely mandate the development of multiplexed EV technologies. Iteratively analyses near single EVs have been challenging to implement beyond a few colors during spectral sensing. Here we developed analysis technique (MASEV) interrogate thousands individual 5 cycles multi-channel fluorescence staining for 15 biomarkers. Contrary common belief, show that: several markers proposed be ubiquitous less prevalent than believed; multiple biomarkers concur in only small fractions; affinity purification can lead loss rare subtypes; deep profiling allows detailed EV, potentially improving diagnostic content. These findings establish potential MASEV uncovering fundamental biology heterogeneity increasing specificity.

Язык: Английский

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Extracellular vesicle–matrix interactions

Nature Reviews Materials, Год журнала: 2023, Номер 8(6), С. 390 - 402

Опубликована: Март 17, 2023

Язык: Английский

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Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Advanced Science, Год журнала: 2024, Номер 11(30)

Опубликована: Июнь 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Язык: Английский

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Construction of Exosome SORL1 Detection Platform Based on 3D Porous Microfluidic Chip and its Application in Early Diagnosis of Colorectal Cancer

Small, Год журнала: 2023, Номер 19(20)

Опубликована: Фев. 17, 2023

Abstract Exosomes are promising new biomarkers for colorectal cancer (CRC) diagnosis, due to their rich biological fingerprints and high level of stability. However, the accurate detection exosomes with specific surface receptors is limited clinical application. Herein, an exosome enrichment platform on a 3D porous sponge microfluidic chip constructed capture efficiency this ≈90%. Also, deep mass spectrometry analysis followed by multi‐level expression screenings revealed CRC‐specific membrane protein (SORL1). A method SORL1 quantum dot labeling further designed ensemble classification system established extracting features from 64‐patched fluorescence images. Importantly, area under curve (AUC) using 0.99, which significantly higher ( p < 0.001) than that conventional biomarker (carcinoembryonic antigen (CEA), AUC 0.71). The above showed similar diagnostic performance, dealing early‐stage CRC, young CEA‐negative CRC patients.

Язык: Английский

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Comprehensive characterization of human brain‐derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications

Journal of Extracellular Vesicles, Год журнала: 2023, Номер 12(8)

Опубликована: Авг. 1, 2023

Abstract Extracellular vesicles (EVs) have emerged as critical mediators of intercellular communication and promising biomarkers therapeutics in the central nervous system (CNS). Human brain‐derived EVs (BDEVs) provide a comprehensive snapshot physiological changes brain's environment, however, isolation BDEVs comparison different methods for this purpose not been fully investigated. In study, we compared yield, morphology, subtypes protein cargo composition isolated from temporal cortex aged human brains using three established separation methods: size‐exclusion chromatography (SEC), phosphatidylserine affinity capture (MagE) sucrose gradient ultracentrifugation (SG‐UC). Our results showed that SG‐UC method provided highest yield collected larger to SEC MagE assessed by transmission electron microscopy nanoparticle tracking analysis (NTA). Quantitative tandem mass‐tag (TMT) mass spectrometry EV samples identified total 1158 proteins, with showing best enrichment common proteins less contamination non‐EV proteins. addition, were enriched associated ATP activity CNS maintenance, abundant neuronal oligodendrocytic molecules. contrast, more molecules related lipoproteins, cell‐substrate junction microglia, whereas highly extracellular matrix, Alzheimer's disease astrocytes. Finally, validated proteomic performing single‐particle super‐resolution flow cytometry. Overall, our findings demonstrate differences size, single assay methods, suggesting choice will significant impact on downstream discovery.

Язык: Английский

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