Biosensors and Bioelectronics, Год журнала: 2024, Номер 266, С. 116718 - 116718
Опубликована: Авг. 30, 2024
Язык: Английский
Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(17)
Опубликована: Янв. 30, 2023
Abstract Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires long turnaround time, expensive equipment, trained personnel, limiting its widespread availability diagnostic capacity. On the other hand, clustered regularly interspaced short palindromic repeats (CRISPR) technology recently demonstrated capability for detection with high sensitivity specificity. CRISPR‐mediated biosensing holds great promise revolutionizing procedures developing point‐of‐care diagnostics. This review focuses on recent developments both fundamental CRISPR biochemistry CRISPR‐based techniques. Four ongoing research hotspots molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid SARS‐CoV‐2 detection‐are also covered.
Язык: Английский
Процитировано
103
ACS Nano, Год журнала: 2024, Номер 18(14), С. 9784 - 9797
Опубликована: Март 12, 2024
Extracellular vesicles (EVs) secreted by all cell types are involved in the cell-to-cell transfer of regulatory factors that influence and tissue phenotypes normal diseased tissues. EVs thus a rich source biomarker targets for assays analyze blood urinary disease diagnosis. Sensitive detection derived from specific populations is key major hurdle when analyzing complex biological samples, but innovative approaches surveyed this Perspective can streamline EV isolation enhance sensitivity procedures required clinical application EV-based diagnostics therapeutics, including nanotechnology microfluidics, to achieve characterizations. Finally, also outlines opportunities challenges remaining translation assays.
Язык: Английский
Процитировано
29
Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(9), С. 2236 - 2252
Опубликована: Янв. 1, 2024
Plant-derived exosomes (PDEs) show promising potential to be developed as a therapeutic agent against cancer, owing their multiple advantages such low toxicity, biocompatibility, availability, affordability, etc.
Язык: Английский
Процитировано
27
Nature Nanotechnology, Год журнала: 2024, Номер unknown
Опубликована: Окт. 28, 2024
Язык: Английский
Процитировано
19
The Journal of Liquid Biopsy, Год журнала: 2024, Номер 3, С. 100138 - 100138
Опубликована: Янв. 4, 2024
Extracellular vesicles (EVs) are the cell's secreted component. It is majorly classified into microvesicles, apoptotic bodies, and exosomes. Exosomes play a significant role in cancer development progression. Its molecular signature (DNA, RNA, Proteins, lipids) has more priority profiling current decade. In prevention, most challenging part early detection. EVs-based screening develops promising platform. Multi-Omics exosomes profiling-based liquid biopsy support detection efficient way. This approach provides detailed expression data (it may be inner cargos or surface express molecules). article highlited multi-omic exosome theranostics applications cancer, technical challenges, improvisation for future improvement.
Язык: Английский
Процитировано
16
Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 199, С. 114974 - 114974
Опубликована: Июнь 24, 2023
Язык: Английский
Процитировано
39
Journal of Cellular and Molecular Medicine, Год журнала: 2023, Номер 27(11), С. 1603 - 1607
Опубликована: Май 15, 2023
This short communication will enlighten the readers about exosome and epithelial-mesenchymal transition (EMT) related to several complicated events. It also highlighted therapeutic potential of exosomes against EMT. Exosome toxicology, heterogeneity, a single profiling approach are covered in this article. In future, could help us get closer cancer vaccine precision oncology.
Язык: Английский
Процитировано
25
ACS Applied Bio Materials, Год журнала: 2023, Номер 6(7), С. 2576 - 2590
Опубликована: Июнь 14, 2023
Cancer is a complex deadly disease that has caused global health crisis in recent epochs. Colorectal cancer (CRC) the third most common malignant gastrointestinal disease. It led to high mortality due early diagnostic failure. Extracellular vesicles (EVs) come with promising solutions for CRC. Exosomes (a subpopulation of EVs) play vital role as signaling molecules CRC tumor microenvironment. secreted from all active cells. Exosome-based molecular transport (DNA, RNA, proteins, lipids, etc.) transforms recipient cell's nature. In CRC, cell-derived exosomes (TEXs) regulate multiple events development and progression such immunogenic suppression, angiogenesis, epithelial–mesenchymal transitions (EMT), physical changes extracellular matrix (ECM), metastasis. Biofluid-circulated tumor-derived are potential tool liquid biopsy. colorectal detection creates great impact biomarker research. The exosome-associated theranostics approach state-of-the-art method. this review, we address associated progression, on screening (diagnostic prognostic biomarkers), also highlight several clinical trials, well future directions exosome-based Hopefully, it will encourage researchers develop theranostic fight
Язык: Английский
Процитировано
25
The Journal of Liquid Biopsy, Год журнала: 2023, Номер 2, С. 100129 - 100129
Опубликована: Ноя. 26, 2023
Cancer is an unsolved health crisis worldwide. Extracellular vesicles (EVs) address this problem in a new way. In cancer, early detection highly challenging, exosomes (a subpopulation of EVs, originating from endosomes) overcome limitation. tumor-derived (TEXs) play role as signaling molecules cancer development and progression. TEXs required more detailed investigation for specific biomarkers research. Exosome heterogeneity (some the facts regulate it such exosome size, origin, inner molecular diversity) made complication liquid biopsy. Single profiling barcoding support overcoming limitation developing precision oncology. interdisciplinary approach screening specifically.
Язык: Английский
Процитировано
25
Clinical and Translational Discovery, Год журнала: 2023, Номер 3(3)
Опубликована: Май 26, 2023
Abstract Extracellular vesicles (EVs) are an innovative orientation of next‐generation cancer medicine. In EVs, the exosome is most exciting domain associated with research current decade.Exosomes have both progression related and healing capabilities depending on source. It develops a new platform for personalized precision medicine visionary project to come reality.
Язык: Английский
Процитировано
24