SIAM Journal on Applied Mathematics, Год журнала: 2025, Номер 85(3), С. 1121 - 1142
Опубликована: Май 15, 2025
Язык: Английский
Journal of Cell Science, Год журнала: 2024, Номер 137(17)
Опубликована: Авг. 9, 2024
Cells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health disease. In single adhesive cells, mechanotransduction involves the transmission of force from extracellular matrix cell nucleus, where it affects nucleocytoplasmic transport (NCT) subsequent nuclear localization transcriptional regulators, such as YAP (also known YAP1). However, if how NCT is mechanosensitive multicellular systems unclear. Here, we characterize use a fluorescent sensor (Sencyt) demonstrate that responds but not density monolayers. Using monolayers both epithelial mesenchymal phenotype, show altered response osmotic shocks inhibition contractility. Furthermore, correlates with degree deformation measured solidity, shape parameter related envelope tension. contrast, sensitive density, showing cell-cell contacts via mere effect NCT. Our results generality regulation
Язык: Английский
Процитировано
4
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Ноя. 22, 2024
Extracellular matrix (ECM) stiffness influences cancer cell fate by altering gene expression. Previous studies suggest that stiffness-induced nuclear deformation may regulate expression through YAP localization. We investigated the role of lamina in this process. show exhibits mechanical threshold behavior: once unwrinkled, is inextensible. A computational model predicts unwrinkled under tension, which confirmed using a lamin tension sensor. Laminar unwrinkling caused flattening during spreading on stiff ECM. Knockdown A/C eliminates surface and decreases These findings cells conforms to drop reveal for controlling localization cells. Matrix induced regulates This study finds induces promotes yes associated protein (YAP), transcriptional co-activator.
Язык: Английский
Процитировано
4
Molecular Cell, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate extreme changes in osmolarity, assess which role pore complexes (NPCs) play achieving volume relieving mechanical stress. capitalize on unique properties D. discoideum quantify fluid flow across NPCs. has an elaborate NPC structure situ. Its dilation state affects permeability nucleocytosolic flow. Based mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous NPCs, is distinct canonically characterized modes nucleocytoplasmic transport because dependence pressure. Viral blockage decreased Our results may be relevant any biological that entail size adaptation, including metastasizing cancer cells, migrating or differentiating tissues.
Язык: Английский
Процитировано
4
Communications Biology, Год журнала: 2025, Номер 8(1)
Опубликована: Фев. 20, 2025
Abstract Cell morphology heterogeneity is pervasive in epithelial collectives, yet the underlying mechanisms driving such and its consequential biological ramifications remain elusive. Here, we observed a consistent correlation between cell nucleus during crowding, revealing persistent log-normal probability distribution characterizing both areas across diverse model systems. We showed that this morphological diversity arises from asymmetric partitioning division. Next, provide insights into impact of on chromatin modifications. demonstrated constraining leads to downregulation euchromatic mark H3K9ac upregulation heterochromatic H3K27me3. Furthermore, size regulates H3K27me3 levels through histone demethylase UTX. These findings highlight significance as driver state diversity, shaping functional variability within tissues.
Язык: Английский
Процитировано
0
SIAM Journal on Applied Mathematics, Год журнала: 2025, Номер 85(3), С. 1121 - 1142
Опубликована: Май 15, 2025
Язык: Английский
Процитировано
0