Progress in Neurobiology, Год журнала: 2024, Номер 235, С. 102600 - 102600
Опубликована: Март 26, 2024
Язык: Английский
Development, Год журнала: 2022, Номер 149(20)
Опубликована: Окт. 15, 2022
ABSTRACT Deconstructing and then reconstructing developmental processes ex vivo is crucial to understanding how organs assemble physiology can be disrupted in disease. Human 3D stem cell-derived systems, such as organoids, have facilitated this pursuit; however, they often do not capture inter-tissue or inter-lineage cellular interactions that give rise emergent tissue properties during development. Assembloids are self-organizing systems result from the integration of multiple organoids combination with missing cell types primary explants. Here, we outline concept assembloids present their applications for studying nervous system other tissues. We describe tools used probe manipulate delineate current challenges potential new approach interrogate development
Язык: Английский
Процитировано
61
Cancer Medicine, Год журнала: 2023, Номер 12(13), С. 14375 - 14386
Опубликована: Апрель 20, 2023
Establishing a valid in vitro model to represent tumor heterogeneity and biology is critical but challenging. Tumor organoids are self-assembled three-dimensional cell clusters which of great significance for recapitulating the histopathological, genetic, phenotypic characteristics primary tissues. The organoid has emerged as an attractive platform research high-throughput drug screening cancer medicine. Organoids offer unique advantages over lines patient-derived xenograft models, there no standardized methods guide culture organoids, leading confusion studies that may affect accurate judgments biology. This review summarizes shortcomings current methods, presents latest findings on standardization, proposes outlook modeling.
Язык: Английский
Процитировано
35
Trends in Molecular Medicine, Год журнала: 2023, Номер 29(8), С. 659 - 672
Опубликована: Июнь 22, 2023
Progression of Alzheimer's disease (AD) entails deterioration or aberrant function multiple brain cell types, eventually leading to neurodegeneration and cognitive decline. Defining how complex cell-cell interactions become dysregulated in AD requires novel human cell-based vitro platforms that could recapitulate the intricate cytoarchitecture diversity brain. Brain organoids (BOs) are 3D self-organizing tissues partially resemble architecture can AD-relevant pathology. In this review, we highlight versatile applications different types BOs model pathogenesis, including amyloid-β tau aggregation, neuroinflammation, myelin breakdown, vascular dysfunction, other phenotypes, as well accelerate therapeutic development for AD.
Язык: Английский
Процитировано
34
Nature reviews. Immunology, Год журнала: 2023, Номер 24(1), С. 18 - 32
Опубликована: Июль 4, 2023
Язык: Английский
Процитировано
32
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(15), С. 12528 - 12528
Опубликована: Авг. 7, 2023
Brain organoids are three-dimensional (3D) structures derived from human pluripotent stem cells (hPSCs) that reflect early brain organization. These contain different cell types, including neurons and glia, similar to those found in the brain. Human provide unique opportunities model features of development not well-reflected animal models. Compared with traditional cultures models, offer a more accurate representation function, rendering them suitable models for neurodevelopmental diseases. In particular, patients’ have enabled researchers study diseases at stages gain better understanding disease mechanisms. Multi-brain regional assembloids allow investigation interactions between distinct regions while achieving higher level consistency molecular functional characterization. Although possess promising features, their usefulness is limited by several unresolved constraints, cellular stress, hypoxia, necrosis, lack high-fidelity maturation, circuit formation. this review, we discuss studies overcome natural limitations organoids, emphasizing importance combinations all neural such as glia (astrocyte, oligodendrocytes, microglia) vascular cells. Additionally, considering similarity developing brain, regionally patterned organoid-derived (NSCs) could serve scalable source replacement therapy. We highlight potential application therapy within field.
Язык: Английский
Процитировано
30
Bioengineering, Год журнала: 2023, Номер 10(2), С. 124 - 124
Опубликована: Янв. 17, 2023
Tissue organoids hold enormous potential as tools for a variety of applications, including disease modeling and drug screening. To effectively mimic the native tissue environment, it is critical to integrate microvasculature with parenchyma stroma. In addition providing means physiologically perfuse organoids, also contributes cellular dynamics model via cells perivascular niche, thereby further modulating function. this review, we discuss current developing strategies vascularizing consider tissue-specific vascularization approaches, importance perfusion, provide perspectives on state field.
Язык: Английский
Процитировано
25
Biological Psychiatry, Год журнала: 2023, Номер 93(7), С. 616 - 621
Опубликована: Янв. 13, 2023
Язык: Английский
Процитировано
24
Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 18
Опубликована: Март 20, 2024
Stem cell-derived organoid technology is a powerful tool that revolutionizes the field of biomedical research and extends scope our understanding human biology diseases. Brain organoids especially open an opportunity for brain modeling many neurological diseases, which have lagged due to inaccessibility samples lack similarity with other animal models. can be generated through various protocols mimic whole or region-specific. To provide overview technology, we summarize currently available list several factors consider before choosing protocols. We also outline limitations current challenges need solved in future investigation development pathobiology.
Язык: Английский
Процитировано
15
Microchimica Acta, Год журнала: 2024, Номер 191(1)
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
13
Lab on a Chip, Год журнала: 2024, Номер 24(4), С. 680 - 696
Опубликована: Янв. 1, 2024
The first global analysis of the cerebral organoid (CO) response to 3D microvasculature indicates elaborate crosstalk between COs and ECs points out potential vascularization factors for brain on a chip.
Язык: Английский
Процитировано
13