Cold Spring Harbor Perspectives in Medicine, Год журнала: 2023, Номер 14(5), С. a041384 - a041384
Опубликована: Июль 24, 2023
Francisco J. Sánchez Rivera1,2 and Lukas E. Dow3,4,5 1David H. Koch Institute for Integrative Cancer Research, Massachusetts of Technology, Cambridge, 02142, USA 2Department Biology, 3Sandra Edward Meyer Center, Weill Cornell Medicine, New York, York 10065, 4Department Biochemistry, 5Department Correspondence: fsr{at}mit.edu; lud2005{at}med.cornell.edu
Язык: Английский
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Ноя. 29, 2024
Abstract Despite its broad application in vitro studies, the of targeted protein degradation (TPD) to animal models faces considerable challenges. Here, we develop inducible and cell-type specific TPD systems mice using two degron systems: Oryza sativa TIR1 F74G (OsTIR1)-auxin-inducible 2 (AID2) human cereblon (hCRBN)-SALL4 (S4D). Efficient Satb1 Venus by these recapitulates phenotypes observed Satb1-deficient mice. These are successfully applied both fetal neonatal stages. The OsTIR1-AID2 system proves be effective for membrane proteins such as PD-1, emulating effects anti-PD-1 antibody. Degradation Bcl11b reveals a role which was not characterized Cre-loxP system. Collectively, vivo technologies developed this study enable inducible, temporal, cell type-specific depletion target with high efficacy have wide range applications diverse fields biological medical research.
Язык: Английский
Процитировано
1
Trends in Immunology, Год журнала: 2023, Номер 44(7), С. 530 - 541
Опубликована: Май 29, 2023
Язык: Английский
Процитировано
3
Essays in Biochemistry, Год журнала: 2022, Номер 66(2), С. 75 - 85
Опубликована: Авг. 1, 2022
Abstract The Green Revolution of the 1960s accomplished dramatic increases in crop yields through genetic improvement, chemical fertilisers, irrigation, and mechanisation. However, current trajectory population growth, against a backdrop climate change geopolitical unrest, predicts that agricultural production will be insufficient to ensure global food security next three decades. Improvements crops go beyond incremental gains are urgently needed. Plant biology has also undergone revolution recent years, development application powerful technologies including genome sequencing, pantheon ‘omics techniques, precise editing, step changes structural microscopy. Proteostasis – collective processes control protein complement cell, comprising synthesis, modification, localisation, degradation is field benefitted from these advances. This special issue presents selection latest research this vibrant field, with particular focus on degradation. In article, we highlight diverse widespread contributions plant proteostasis agronomic traits, suggest opportunities strategies manipulate different elements proteostatic mechanisms for discuss challenges involved bringing ideas into practice.
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(7), С. 6526 - 6526
Опубликована: Март 30, 2023
Optogenetic systems driven by yellow-orange light are required for the simultaneous regulation of several cellular processes. We have engineered red fluorescent protein FusionRed into a 26 kDa monomeric optogenetic module, called degFusionRed. Unlike other protein-based domains, which exhibit light-induced self-inactivation generating reactive oxygen species, degFusionRed undergoes proteasomal degradation upon illumination with 567 nm light. Similarly to parent protein, has minimal absorbance at 450 and above 650 nm, making it spectrally compatible blue near-infrared-light-controlled tools. The autocatalytically formed chromophore provides an additional advantage over most tools that require binding exogenous chromophores, amount varies in different cells. efficiently performed light-controlled transcription factor targeted photodegradation interest, demonstrating its versatility as module choice spectral multiplexed interrogation various
Язык: Английский
Процитировано
2
Cold Spring Harbor Perspectives in Medicine, Год журнала: 2023, Номер 14(5), С. a041384 - a041384
Опубликована: Июль 24, 2023
Francisco J. Sánchez Rivera1,2 and Lukas E. Dow3,4,5 1David H. Koch Institute for Integrative Cancer Research, Massachusetts of Technology, Cambridge, 02142, USA 2Department Biology, 3Sandra Edward Meyer Center, Weill Cornell Medicine, New York, York 10065, 4Department Biochemistry, 5Department Correspondence: fsr{at}mit.edu; lud2005{at}med.cornell.edu
Язык: Английский
Процитировано
2