bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Март 10, 2023
The
prefrontal
cortex
(PFC)
regulates
drinking
behaviors
and
affective
changes
following
chronic
alcohol
use.
PFC
activity
is
dynamically
modulated
by
local
inhibitory
interneurons
(INs),
which
can
be
divided
into
non-overlapping
groups
with
distinct
functional
roles.
Within
deeper
layers
of
neocortex,
INs
that
express
either
parvalbumin
or
somatostatin
directly
inhibit
pyramidal
cells.
By
contrast,
the
plurality
all
remaining
vasoactive
intestinal
peptide
(VIP),
reside
within
superficial
layers,
preferentially
target
other
types
INs.
While
recent
studies
have
described
adaptations
to
parvalbumin-INs
somatostatin-INs
in
use
models,
whether
ethanol
affect
physiology
VIP-INs
has
not
been
reported.
To
address
this
gap,
we
used
genetically
engineered
female
male
mice
1-3
prelimbic
for
whole-cell
patch-clamp
electrophysiology.
We
found
(20
mM,
∼0.09
BEC)
application
brain
slices
enhances
VIP-IN
excitability.
next
examined
effects
providing
4
weeks
intermittent
access
(IA)
two-bottle
choice
home
cage.
In
these
studies,
from
IA
displayed
reduced
excitability
relative
cells
water-only
controls.
Finally,
assessed
continue
abstinence.
After
7-11
days
without
ethanol,
hypo-excitability
persisted,
whereas
were
different
their
Together,
findings
illustrate
acute
suggest
undergo
pronounced
homeostatic
long-term
drinking.
Cell Reports,
Год журнала:
2023,
Номер
42(9), С. 113133 - 113133
Опубликована: Сен. 1, 2023
Visual
stimuli
that
deviate
from
the
current
context
elicit
augmented
responses
in
primary
visual
cortex
(V1).
These
heightened
responses,
known
as
"deviance
detection,"
require
local
inhibition
V1
and
top-down
input
anterior
cingulate
area
(ACa).
Here,
we
investigated
mechanisms
by
which
ACa
interact
to
support
deviance
detection.
Local
field
potential
recordings
mice
during
an
oddball
paradigm
showed
ACa-V1
synchrony
peaks
theta/alpha
band
(≈10
Hz).
Two-photon
imaging
revealed
mainly
pyramidal
neurons
exhibited
detection,
while
contextually
redundant
increased
vasoactive
intestinal
peptide
(VIP)-positive
interneuron
(VIP)
activity
decreased
somatostatin-positive
(SST)
activity.
Optogenetic
drive
of
inputs
at
10
Hz
activated
V1-VIPs
but
inhibited
V1-SSTs,
mirroring
dynamics
present
paradigm.
Chemogenetic
disrupted
Aca-V1
detection
V1.
results
outline
temporal
interneuron-specific
modulation
processing.
Nature,
Год журнала:
2024,
Номер
633(8029), С. 398 - 406
Опубликована: Авг. 28, 2024
Abstract
The
brain
functions
as
a
prediction
machine,
utilizing
an
internal
model
of
the
world
to
anticipate
sensations
and
outcomes
our
actions.
Discrepancies
between
expected
actual
events,
referred
errors,
are
leveraged
update
guide
attention
towards
unexpected
events
1–10
.
Despite
importance
prediction-error
signals
for
various
neural
computations
across
brain,
surprisingly
little
is
known
about
circuit
mechanisms
responsible
their
implementation.
Here
we
describe
thalamocortical
disinhibitory
that
required
generating
sensory
in
mouse
primary
visual
cortex
(V1).
We
show
violating
animals’
predictions
by
stimulus
preferentially
boosts
responses
layer
2/3
V1
neurons
most
selective
stimulus.
Prediction
errors
specifically
amplify
input,
rather
than
representing
non-specific
surprise
or
difference
how
input
deviates
from
animal’s
predictions.
This
amplification
implemented
cooperative
mechanism
requiring
thalamic
pulvinar
cortical
vasoactive-intestinal-peptide-expressing
(VIP)
inhibitory
interneurons.
In
response
VIP
inhibit
specific
subpopulation
somatostatin-expressing
interneurons
gate
excitatory
V1,
resulting
pulvinar-driven
stimulus-selective
V1.
Therefore,
prioritizes
unpredicted
information
selectively
increasing
salience
features
through
synergistic
interaction
neocortical
circuits.
Cell Reports,
Год журнала:
2024,
Номер
43(4), С. 114115 - 114115
Опубликована: Апрель 1, 2024
In
the
CA1
hippocampus,
vasoactive
intestinal
polypeptide-expressing
interneurons
(VIP-INs)
play
a
prominent
role
in
disinhibitory
circuit
motifs.
However,
specific
behavioral
conditions
that
lead
to
disinhibition
remain
uncertain.
To
investigate
relevance
of
VIP-IN
activity,
we
employed
wireless
technologies
allowing
us
monitor
and
manipulate
their
function
freely
behaving
mice.
Our
findings
reveal
that,
during
spatial
exploration
new
environments,
VIP-INs
hippocampal
region
become
highly
active,
facilitating
rapid
encoding
novel
information.
Remarkably,
both
pyramidal
neurons
(PNs)
exhibit
increased
activity
when
encountering
changes
environment,
including
context-
object-related
alterations.
Concurrently,
somatostatin-
parvalbumin-expressing
inhibitory
populations
show
an
inverse
relationship
with
PN
revealing
occurs
on
timescale
seconds.
Thus,
VIP-IN-mediated
may
constitute
crucial
element
novelty
acquisition
recognition
memory.
Cell Reports,
Год журнала:
2023,
Номер
42(9), С. 113088 - 113088
Опубликована: Сен. 1, 2023
Cortical
circuit
function
is
regulated
by
extensively
interconnected,
diverse
populations
of
GABAergic
interneurons
that
may
play
key
roles
in
shaping
operation
according
to
behavioral
context.
A
specialized
population
co-express
vasoactive
intestinal
peptides
(VIP-INs)
are
activated
during
arousal
and
innervate
other
INs
pyramidal
neurons
(PNs).
Although
state-dependent
modulation
VIP-INs
has
been
studied,
their
role
regulating
sensory
processing
less
well
understood.
We
examined
the
impact
primary
visual
cortex
awake
behaving
mice.
Loss
VIP-IN
activity
alters
somatostatin-expressing
(SST-INs)
but
not
PNs.
In
contrast,
reduced
globally
disrupts
feature
selectivity
for
stimulus
size.
Moreover,
on
perceptual
behavior
varies
with
context
more
acute
small
than
large
cues.
thus
contribute
both
cortical
context-dependent
performance.
Current Biology,
Год журнала:
2023,
Номер
33(16), С. 3398 - 3408.e7
Опубликована: Июль 26, 2023
Vasoactive
intestinal
peptide
(VIP)
interneurons
in
sensory
cortex
modulate
responses
based
on
global
exploratory
behavior
and
arousal
state,
but
their
function
during
non-exploratory,
goal-directed
is
not
well
understood.
In
particular,
whether
VIP
cells
are
activated
by
cues,
reward-seeking
actions,
or
directly
reinforcement
unclear.
We
trained
mice
a
Go/NoGo
whisker
touch
detection
task
that
included
delay
period
other
features
designed
to
separate
sensory-evoked,
action-related,
reward-related
neural
activity.
Mice
had
lick
response
stimulus
receive
variable-sized
reward.
Using
two-photon
calcium
imaging,
we
measured
ΔF/F
of
L2/3
neurons
somatosensory
(S1)
behavior.
both
expert
novice
mice,
were
strongly
stimuli
actions
(licking),
reinforcement.
showed
somatotopic
tuning
was
spatially
organized
relative
anatomical
columns
S1,
unlike
lick-related
signals
which
widespread.
suppressed,
enhanced,
when
reward
delivered,
the
amount
suppression
increased
with
size.
This
seen
where
delivery
yoked
licking.
These
results
indicate
besides
state
variables,
local
Instead,
our
consistent
role
for
encoding
expectation
associated
motor
actions.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 12, 2023
Abstract
The
brain
functions
as
a
prediction
machine,
utilizing
an
internal
model
of
the
world
to
anticipate
sensations
and
outcomes
our
actions.
Discrepancies
between
expected
actual
events,
referred
errors,
are
leveraged
update
guide
attention
towards
unexpected
events
1–10
.
Despite
importance
error
signals
for
various
neural
computations
across
multiple
regions,
surprisingly
little
is
known
about
circuit
mechanisms
responsible
their
implementation.
Here
we
describe
thalamocortical
disinhibitory
required
generating
sensory
errors
in
mouse
primary
visual
cortex
(V1).
Using
calcium
imaging
with
optogenetic
manipulations
mice
traverse
familiar
virtual
environment,
show
that
violation
animals’
predictions
by
stimulus
preferentially
boosts
responses
layer
2/3
V1
neurons
most
selective
stimulus.
Prediction
specifically
amplify
input,
rather
than
representing
non-specific
surprise
or
difference
signal
how
input
deviates
from
predictions.
Selective
amplification
implemented
cooperative
mechanism
requiring
thalamic
pulvinar,
cortical
vasoactive-intestinal-peptide-expressing
(VIP)
inhibitory
interneurons.
In
response
VIP
inhibit
specific
subpopulation
somatostatin-expressing
(SOM)
interneurons
gate
excitatory
pulvinar
V1,
resulting
pulvinar-driven
response-amplification
stimulus-selective
V1.
Therefore,
prioritizes
unpredicted
information
selectively
increasing
salience
features
through
synergistic
interaction
neocortical
circuits.
Neurobiology of Disease,
Год журнала:
2025,
Номер
211, С. 106931 - 106931
Опубликована: Апрель 30, 2025
Nutritional
psychiatry
has
gained
increasing
attention,
particularly
in
exploring
dietary
interventions
for
depression
treatment.
As
a
potential
non-drug
intervention,
intermittent
fasting
(IF)
gradually
attracted
the
interest
of
researchers,
but
its
specific
neurobiological
mechanisms
remain
unclear.
The
medial
prefrontal
cortex
(mPFC)
dopamine
D1
receptors
(Drd1)
are
significant
stress
response
and
serve
as
molecular
target
rapid-acting
antidepressants.
Our
previous
study
indicated
that
9-h
produces
an
antidepressant-like
effect
by
modulating
(DA)
receptors.
However,
whether
IF
effects
through
actions
on
DA
receptor-mediated
remains
Here,
we
investigated
improving
depression-like
behavior
induced
Chronic
Unpredictable
Mild
Stress
(CUMS)
mice
explored
these
regulated
Drd1.
We
found
alleviated
CUMS-induced
behavior,
increased
c-Fos
expression
mPFC
hippocampus
CUMS
mice,
activated
Drd1-cAMP-PKA-DARPP-32-CREB-BDNF
signaling
pathway.
were
reversed
Drd1
antagonist
SCH23390.
Additionally,
optogenetic
activation
Drd1-expressing
neurons
improved
while
inhibition
suppressed
IF-induced
effects.
These
findings
imply
plays
crucial
role
offer
valuable
insights
into
application
clinical