Odor-regulated oviposition behavior in an ecological specialist

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 26, 2023

Abstract Colonization of a novel ecological niche can require, or be driven by, evolution an animal’s behaviors promoting their reproductive success. We investigated the and sensory basis oviposition in Drosophila sechellia , close relative melanogaster that exhibits extreme specialism for Morinda citrifolia noni fruit. D. produces fewer eggs than other drosophilids lays these almost exclusively on substrates. show visual, textural social cues do not explain this species-specific preference. By contrast, we find loss olfactory input but essentially abolishes egg-laying, suggesting olfaction gates gustatory-driven Noni odors are detected by redundant pathways, discover role hexanoic acid cognate Ionotropic receptor 75b (Ir75b) odor-evoked oviposition. Through exchange provide evidence causal contribution odor-tuning changes Ir75b to ’s behavior.

Язык: Английский

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Sugar sensation and mechanosensation in the egg-laying preference shift of Drosophila suzukii

eLife, Год журнала: 2022, Номер 11

Опубликована: Ноя. 18, 2022

The agricultural pest Drosophila suzukii differs from most other species in that it lays eggs ripe, rather than overripe, fruit. Previously, we showed changes bitter taste sensation accompanied this adaptation (Dweck et al., 2021). Here, show D. has also undergone a variety of sweet sensation. weaker preference melanogaster for laying on substrates containing all three primary fruit sugars: sucrose, fructose, and glucose. Major subsets sensilla have lost electrophysiological responses to sugars. Expression several key sugar receptor genes is reduced the organs suzukii. By contrast, certain mechanosensory channel genes, including no mechanoreceptor potential C, are expressed at higher levels suzukii, which stiff substrates. Finally, find responds differently combinations cues. Thus, two differ sensation, mechanosensation, their integration, likely contribute differences egg-laying preferences nature.

Язык: Английский

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Increased sugar valuation contributes to the evolutionary shift in egg-laying behavior of the fruit pest Drosophila suzukii

PLoS Biology, Год журнала: 2023, Номер 21(12), С. e3002432 - e3002432

Опубликована: Дек. 11, 2023

Behavior evolution can promote the emergence of agricultural pests by changing their ecological niche. For example, insect pest Drosophila suzukii has shifted its oviposition (egg-laying) niche from fermented fruits to ripe, non-fermented fruits, causing significant damage a wide range fruit crops worldwide. We investigate chemosensory changes underlying this evolutionary shift and ask whether sugars, which are depleted during fermentation, important gustatory cues that direct D. sweet, ripe fruits. show expanded responses lower sugar concentrations than model melanogaster, prefers lay eggs on fruit. The increased response correlates with an increase in value relative strawberry substrate decisions. In addition, we genetic manipulation sugar-gustatory receptor neurons (GRNs) perception is required for prefer over substrate, but not melanogaster substrate. Thus, major determinant suzukii's choice complex substrates. Calcium imaging experiments brain's primary center (suboesophageal zone) GRNs more sensitive counterparts, suggesting valuation encoded downstream circuits central nervous system (CNS). Taken together, our data suggest brain computations involved driving preference

Язык: Английский

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Connectomic analysis of taste circuits in Drosophila

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 12, 2025

Abstract Our sense of taste is critical for regulating food consumption. The fruit fly Drosophila represents a highly tractable model to investigate mechanisms processing, but circuits beyond sensory neurons are largely unidentified. Here, we use whole-brain connectome the organization circuits. We trace pathways from four populations that detect different modalities and project subesophageal zone (SEZ), primary region brain. find second-order primarily located within SEZ segregated by modality, whereas third-order have more projections outside overlap between modalities. Taste out innervate regions implicated in feeding, olfactory learning. analyze interconnections pathways, characterize modality-dependent differences neuron properties, identify other types inputs onto computational simulations relate neuronal connectivity predicted activity. These studies provide insight into architecture

Язык: Английский

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Functional characterization and evolution of olfactory responses in coeloconic sensilla of the global fruit pest Drosophila suzukii

BMC Biology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 21, 2025

Язык: Английский

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Jasmonic acid and salicylic acid induced defensive response in wine grapes against Drosophila suzukii (Diptera: Drosophilidae)

Heliyon, Год журнала: 2023, Номер 9(6), С. e16505 - e16505

Опубликована: Май 21, 2023

To better understand the elicitor induced defense in wine grapes against Drosophila suzukii (Matsumura), two varieties, Pinot Noir and Chardonnay, were sprayed with jasmonic acid (JA) salicylic (SA). Total phenols, total flavonoids, tannins soluble sugars quantified. Oviposition preference by D. response to JA SA applications also studied. Behavioral of various was recorded. The effect flavonoids (gallic acid, catechin, quercetin at 100 500 ppm) on mortality studied CAFE assay. Our results showed that application significantly affected phenol, flavonoid tannin contents grapes. Reduced injury observed treated plants; this reduction more pronounced Chardonnay than Noir. number eggs laid females lower SA-treated under no-choice conditions choice conditions. In prandial behavior, sucrose solution (5%), glucose fructose (5%) + yeast solutions higher attraction compared control treatments. Among catechin (100 rest study can be used for developing management strategies related crops.

Язык: Английский

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An advanced metabolomic approach on grape skins untangles cultivar preferences by Drosophila suzukii for oviposition

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Авг. 21, 2024

Insects’ host preferences are regulated by multiple factors whose interactions only partly understood. Here we make use of an in-depth, untargeted metabolomic approach combining molecular networking (MN) and supervised Analysis variance Multiblock Orthogonal Partial Least Squares (AMOPLS) to untangle egg-laying Drosophila suzukii , invasive, highly polyphagous destructive fruit pest originating from Southeast Asia. Based on behavioural experiments in the laboratory as well field observation, selected eight genetically related Vitis vinifera cultivars (e.g., Ancellotta, Galotta, Gamaret, Gamay, Gamay précoce, Garanoir, Mara Reichensteiner) exhibiting significant differences their susceptibility toward D. . The two most least attractive red were chosen for further analyses grape skins. combination MN statistical AMOPLS findings with semi-quantitative detection information enabled us identify flavonoids interesting markers attractiveness four studied towards Overall, dihydroflavonols accumulated unattractive cultivars, while richer flavonols. Crucially, both flavonols abundant metabolites analysis extracted molecules skin. We discuss how these flavonoid classes might influence behaviour females they could serve potential infestations grapes that can be potentially extended other fruits. believe our novel, integrated analytical also applied study biological relationships characterised evolving parameters.

Язык: Английский

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Mosquito taste responses to human and floral cues guide biting and feeding

Nature, Год журнала: 2024, Номер 635(8039), С. 639 - 646

Опубликована: Окт. 16, 2024

Язык: Английский

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Drosophila HCN mediates gustatory homeostasis by preserving sensillar transepithelial potential in sweet environments

eLife, Год журнала: 2024, Номер 13

Опубликована: Июль 29, 2024

Establishing transepithelial ion disparities is crucial for sensory functions in animals. In insect organs called sensilla, a potential, known as the sensillum potential (SP), arises through active transport across accessory cells, sensitizing receptor neurons such mechanoreceptors and chemoreceptors. Because multiple are often co-housed share SP, niche-prevalent overstimulation of single can compromise neighboring receptors by depleting SP. However, how depletion prevented to maintain homeostasis remains unknown. Here, we find that Ih- encoded hyperpolarization-activated cyclic nucleotide-gated (HCN) channel bolsters activity bitter-sensing gustatory (bGRNs), albeit acting sweet-sensing GRNs (sGRNs). For this task, HCN maintains SP despite prolonged sGRN stimulation induced diet mimicking their sweet feeding niche, overripe fruit. We present evidence Ih -dependent demarcation excitability implemented throttle consumption, which may have facilitated adaptation sweetness-dominated environment. Thus, expressed sGRNs serves key component simple yet versatile peripheral coding regulates bitterness optimal food intake two contrasting ways: sweet-resilient preservation bitter aversion previously reported sweet-dependent suppression taste.

Язык: Английский

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Insulin Signaling Pathway Mediates FoxO–Pepck Axis Regulation of Glucose Homeostasis in Drosophila suzukii

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10441 - 10441

Опубликована: Сен. 27, 2024

The agricultural pest Drosophila suzukii exhibits a strong preference for feeding on fresh fruits, demonstrating high adaptability to sugary environments. Meanwhile, sugar levels stimulate insulin secretion, thereby regulating the steady state of metabolism. Understanding mechanisms related metabolism in D. is crucial due its adaptation these specific environmental conditions. signaling pathway an evolutionarily conserved phosphorylation cascade with significant roles development and We observed that activation inhibited FoxO activity downregulated expression Pepck, activating glycolysis reducing glucose levels. By contrast, inhibiting increased upregulated which activated gluconeogenesis led Our findings demonstrated role mediating through FoxO–Pepck axis, supports ecological high-sugar niches, providing insights into metabolic control suggesting potential strategies management. Elucidating molecular processes important understanding regulation specialization suzukii.

Язык: Английский

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