bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Фев. 25, 2023
Spermatogenesis in the Drosophila male germline proceeds through a unique transcriptional program controlled both by germline-specific transcription factors and testis-specific versions of core machinery. This includes activation genes on heterochromatic
Язык: Английский
eLife, Год журнала: 2023, Номер 12
Опубликована: Фев. 16, 2023
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types, mapping intermediate steps differentiation, potential identify new factors impacting fertility or controlling supporting somatic cells. We justify assignment key types using combinations known markers, situ hybridization, extant protein traps. Comparison datasets proved particularly revealing dynamic developmental transitions differentiation. To complement web-based portals hosted by FCA, compatible with commonly used software such as Seurat Monocle. The foundation provided here will enable communities studying interrogate candidate genes test function vivo.
Язык: Английский
Процитировано
46
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across
Язык: Английский
Процитировано
1
eLife, Год журнала: 2023, Номер 12
Опубликована: Дек. 21, 2023
In Drosophila melanogaster and other insects, the seminal fluid proteins (SFPs) male sex pheromones that enter female with sperm during mating are essential for fertility induce profound post-mating effects on physiology. The SFPs in D. taxa include several members of large gene family known as odorant binding (Obps). Work has shown some Obp genes highly expressed antennae can mediate behavioral responses to odorants, potentially by carrying these molecules receptors. These observations have led hypothesis Obps might act molecular carriers or compounds important fertility, though functional evidence any species is lacking. Here, we used genetics test role seven response (PMR). We found Obp56g required induction PMR, whereas six dispensable. males lacking fail form a plug mated female's reproductive tract, leading ejaculate loss reduced storage, likely due its expression ejaculatory bulb. also examined evolutionary history genes, studies documented rapid evolution turnover SFP across taxa. extensive lability copy number positive selection acting two Obp22a Obp51a. Comparative RNAseq data from tract multiple revealed shows high subset taxa, conserved head phylogeny. Together, suggest may been co-opted function over time.
Язык: Английский
Процитировано
13
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Май 7, 2024
Unlike coding genes, the number of lncRNA genes in organism genomes is relatively proportional to complexity. From plants humans, tissues with highest numbers and levels gene expression are male reproductive organs. To learn why, we initiated a genome-wide analysis Drosophila spatial patterns these tissues. The observed greatly exceed those previously reported, due largely preponderance non-polyadenylated transcripts. In stark contrast lncRNAs expressed post-meiotic spermatids. Correlations between levels, localization performed genetic analyses indicate high function requirement. More focused that play major roles evolution by controlling transposable element activities, Y chromosome sperm construction. A new type lncRNA-based particle found seminal fluid may also contribute outcomes.
Язык: Английский
Процитировано
5
Genome Biology and Evolution, Год журнала: 2024, Номер 16(3)
Опубликована: Март 1, 2024
Abstract Spermatogenesis is critical to sexual reproduction yet evolves rapidly in many organisms. High-throughput single-cell transcriptomics promises unparalleled insight into this important process but understanding can be impeded nonmodel systems by a lack of known genes that reliably demarcate biologically meaningful cell populations. Tribolium castaneum, the red flour beetle, lacks markers for spermatogenesis found insect species like Drosophila melanogaster. Using sequencing data collected from adult beetle testes, we implement strategy elucidating populations using transient expression stage identification markers, weighted principal component clustering, and SNP-based haploid/diploid phasing. We identify correspond observable points sperm differentiation find specific each stage. Our results indicate molecular pathways underlying Coleoptera are substantially diverged those Diptera. also show most on X chromosome experience meiotic sex inactivation. Temporal MSL complex homologs coupled with spatial analysis potential chromatin entry sites further suggests dosage compensation machinery may mediate escape inactivation postmeiotic reactivation chromosome.
Язык: Английский
Процитировано
3
Nucleus, Год журнала: 2024, Номер 15(1)
Опубликована: Апрель 9, 2024
Species' continuity depends on gametogenesis to produce the only cell types that can transmit genetic information across generations. Spermiogenesis, which encompasses post-meiotic, haploid stages of male gametogenesis, is a process leads formation sperm cells well-known for their motility. Spermiogenesis faces three major challenges. First, after two rounds meiotic divisions, genome lacks repair templates (no sister chromatids, no homologous chromosomes), making it incredibly vulnerable any genomic insults over an extended time (typically days-weeks). Second, becomes transcriptionally silent, difficult respond new perturbations as spermiogenesis progresses. Third, histone-to-protamine transition, essential package genome, counterintuitively involves DNA break formation. How handles these challenges remains poorly understood. In this review, we discuss each challenge and intersection with biology protamines. Finally, implication protamines in evolution.
Язык: Английский
Процитировано
3
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Апрель 29, 2024
Spermatogenesis is a key developmental process underlying the origination of newly evolved genes. However, rapid cell type-specific transcriptomic divergence
Язык: Английский
Процитировано
3
eLife, Год журнала: 2025, Номер 13
Опубликована: Янв. 3, 2025
Niches are often found in specific positions tissues relative to the stem cells they support. Consistency of niche position suggests that placement is important for function. However, complexity most niches has precluded a thorough understanding how their proper established. To address this, we investigated formation genetically tractable niche, Drosophila Posterior Signaling Center (PSC), assembly which had not been previously explored. This controls hematopoietic progenitors lymph gland (LG). PSC were shown be specified laterally embryo, but ultimately reside dorsally, at LG posterior. Here, using live-imaging, show migrate as tight collective and associate with multiple during trajectory We find Slit emanating from two extrinsic sources, visceral mesoderm cardioblasts, required remain collective, its attachment cardioblasts migration. Without Slit-Robo signaling, disperse, form aberrant contacts, fail reach stereotypical near progenitors. Our work characterizes novel example identifies an signaling relay precise positioning.
Язык: Английский
Процитировано
0
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 4, 2025
Abstract Preserving a large number of essential yet highly unstable ribosomal DNA (rDNA) repeats is critical for the germline to perpetuate genome through generations. Spontaneous rDNA loss must be countered by copy (CN) expansion. Germline CN expansion best understood in Drosophila melanogaster , which relies on unequal sister chromatid exchange (USCE) initiated breaks at rDNA. The rDNA-specific retrotransposon R2 responsible USCE-inducing typically expressed only when low minimize danger breaks; however, underlying mechanism regulation remains unclear. Here we identify insulin receptor (InR) as major repressor expression, limiting unnecessary activity. Through single-cell RNA sequencing, find that male stem cells (GSCs), cell type undergoes expansion, have reduced InR expression low. Reduced activity turn leads and We further dietary manipulation alters This work reveals pathway integrates surveying with environmental sensing, revealing potential diet exerts heritable changes genomic content.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 11, 2025
SUMMARY In many organisms including Drosophila and mammals, male germ cells, particularly spermatocytes, have one of the most complex transcriptomes, where a large fraction genome noncoding DNA are transcribed. However, significance this widespread transcription remains enigmatic. Through study satellite in spermatocytes as representational model for transcription, we propose that is required to remodel chromatin preparation sperm packaging. We identified HP2, known heterochromatin protein, being AAGAG DNA. HP2 depletion led retention marks post-meiotic spermatids subsequent failure histone-to-protamine transition. Strikingly, with less can develop normally even when perturbed by depletion. Together, spermatocytes’ serves mechanism facilitate remodeling prepare
Язык: Английский
Процитировано
0