Cholinergic input to mouse visual cortex signals a movement state and acutely enhances layer 5 responsiveness

eLife, Год журнала: 2023, Номер 12

Опубликована: Сен. 25, 2023

Acetylcholine is released in visual cortex by axonal projections from the basal forebrain. The signals conveyed these and their computational significance are still unclear. Using two-photon calcium imaging behaving mice, we show that forebrain cholinergic axons mouse provide a binary locomotion state signal. In axons, found no evidence of responses to stimuli or visuomotor prediction errors. While optogenetic activation isolation did not drive local neuronal activity, when paired with stimuli, it resulted layer-specific increases activity. Responses layer 5 neurons both top-down bottom-up inputs were increased amplitude decreased latency, whereas those 2/3 remained unchanged. opto- chemogenetic manipulations acetylcholine underlie locomotion-associated decorrelation activity between 5. Our results suggest augments responsiveness outside network, possibly enabling faster switching internal representations during locomotion.

Язык: Английский

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Flexible neural population dynamics govern the speed and stability of sensory encoding in mouse visual cortex

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 30, 2024

Time courses of neural responses underlie real-time sensory processing and perception. How these temporal dynamics change may be fundamental to how systems adapt different perceptual demands. By simultaneously recording from hundreds neurons in mouse primary visual cortex, we examined population stimuli at sub-second timescales, during behavioural states. We discovered that active states characterised by locomotion, single-neurons shift transient sustained response modes, facilitating rapid emergence stimulus tuning. Differences single-neuron were associated with changes correlations, including faster stabilisation stimulus-evoked the structure correlations locomotion. Using Factor Analysis, latent trajectories activity make more direct transitions between baseline stimulus-encoding This could partly explained dampening oscillatory present stationary Functionally, collectively enabled faster, stable efficient encoding new information These findings reveal a principle demands, where flexible govern speed stability encoding.

Язык: Английский

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Quantification of the effect of hemodynamic occlusion in two-photon imaging

Опубликована: Янв. 6, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Язык: Английский

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Quantification of the effect of hemodynamic occlusion in two-photon imaging

Опубликована: Янв. 6, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Язык: Английский

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Multimodal mismatch responses in mouse auditory cortex

Опубликована: Янв. 28, 2025

Our movements result in predictable sensory feedback that is often multimodal. Based on deviations between predictions and actual input, primary areas of cortex have been shown to compute sensorimotor prediction errors. How errors one modality influence the computation another still unclear. To investigate multimodal mouse auditory (ACx), we used a virtual environment experimentally couple running both self-generated visual feedback. Using two-photon microscopy, first characterized responses layer 2/3 (L2/3) neurons sounds, stimuli, onsets found all three stimuli. Probing evoked by audiomotor mismatches, they closely resemble visuomotor mismatch (V1). Finally, testing for cross modal coupling sound amplitude flow speed running, were amplified when paired with concurrent mismatches. results demonstrate non-hierarchical interactions shape error cortical L2/3.

Язык: Английский

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Multimodal mismatch responses in mouse auditory cortex

eLife, Год журнала: 2025, Номер 13

Опубликована: Фев. 10, 2025

Our movements result in predictable sensory feedback that is often multimodal. Based on deviations between predictions and actual input, primary areas of cortex have been shown to compute sensorimotor prediction errors. How errors one modality influence the computation another still unclear. To investigate multimodal mouse auditory cortex, we used a virtual environment experimentally couple running both self-generated visual feedback. Using two-photon microscopy, first characterized responses layer 2/3 (L2/3) neurons sounds, stimuli, onsets found all three stimuli. Probing evoked by audiomotor (AM) mismatches, they closely resemble visuomotor (VM) mismatch (V1). Finally, testing for cross modal AM coupling sound amplitude flow speed running, were amplified when paired with concurrent VM mismatches. results demonstrate non-hierarchical interactions shape error cortical L2/3.

Язык: Английский

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Quantification of the effect of hemodynamic occlusion in two-photon imaging

Опубликована: Март 31, 2025

The last few years have seen an explosion in the number of tools available to measure neuronal activity using fluorescence imaging (Chen et al., 2013; Feng 2019; Jing Sun 2018; Wan 2021). When performed vivo, these measurements are invariably contaminated by hemodynamic occlusion artifacts. In widefield calcium imaging, this problem is well recognized. For two-photon however, effects only been sparsely characterized. Here we perform a quantification changes observed with GFP expression both and imaging. We find that many instances magnitude signal attributable comparable sensors. Moreover, were spatially heterogeneous, over cortical regions across depth, exhibited complex relationship behavior. Thus, important caveat consider when analyzing interpreting not just but also data.

Язык: Английский

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Multimodal mismatch responses in mouse auditory cortex

Опубликована: Март 22, 2024

Our movements result in predictable sensory feedback that is often multimodal. Based on deviations between predictions and actual input, primary areas of cortex have been shown to compute sensorimotor prediction errors. How errors one modality influence the computation another still unclear. To investigate multimodal mouse auditory (ACx), we used a virtual environment experimentally couple running both self-generated visual feedback. Using two-photon microscopy, first characterized responses layer 2/3 (L2/3) neurons sounds, stimuli, onsets found all three stimuli. Probing evoked by audiomotor mismatches, they closely resemble visuomotor mismatch (V1). Finally, testing for cross modal coupling sound amplitude flow speed running, were amplified when paired with concurrent mismatches. results demonstrate non-hierarchical interactions shape error cortical L2/3.

Язык: Английский

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MULTIMODAL MISMATCH RESPONSES IN MOUSE AUDITORY CORTEX

Опубликована: Ноя. 18, 2024

Our movements result in predictable sensory feedback that is often multimodal. Based on deviations between predictions and actual input, primary areas of cortex have been shown to compute sensorimotor prediction errors. How errors one modality influence the computation another still unclear. To investigate multimodal mouse auditory (ACx), we used a virtual environment experimentally couple running both self-generated visual feedback. Using two-photon microscopy, first characterized responses layer 2/3 (L2/3) neurons sounds, stimuli, onsets found all three stimuli. Probing evoked by audiomotor mismatches, they closely resemble visuomotor mismatch (V1). Finally, testing for cross modal coupling sound amplitude flow speed running, were amplified when paired with concurrent mismatches. results demonstrate non-hierarchical interactions shape error cortical L2/3. *** Dear reader, please note this manuscript formatted standard submission format, statistical information provided Table S1.

Язык: Английский

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MULTIMODAL MISMATCH RESPONSES IN MOUSE AUDITORY CORTEX

Опубликована: Март 23, 2024

Our movements result in predictable sensory feedback that is often multimodal. Based on deviations between predictions and actual input, primary areas of cortex have been shown to compute sensorimotor prediction errors. How errors one modality influence the computation another still unclear. To investigate multimodal mouse auditory cortex, we used a virtual environment experimentally couple running both self-generated visual feedback. Using two-photon microscopy, first characterized responses layer 2/3 (L2/3) neurons sounds, stimuli, onsets found all three stimuli. Probing evoked by audiomotor (AM) mismatches, they closely resemble visuomotor (VM) mismatch (V1). Finally, testing for cross modal AM coupling sound amplitude flow speed running, were amplified when paired with concurrent VM mismatches. results demonstrate non-hierarchical interactions shape error cortical L2/3.

Язык: Английский

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