Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes DOI Creative Commons
Linda S Rubio, Suman Mohajan, David S. Gross

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Ноя. 22, 2023

In insects and mammals, 3D genome topology has been linked to transcriptional states yet whether this link holds for other eukaryotes is unclear. Using both ligation proximity fluorescence microscopy assays, we show that in Saccharomyces cerevisiae , Heat Shock Response ( HSR ) genes dispersed across multiple chromosomes under the control of Factor (Hsf1) rapidly reposition cells exposed acute ethanol stress engage concerted, Hsf1-dependent intergenic interactions. Accompanying reconfiguration equally rapid formation Hsf1-containing condensates. However, contrast transience Hsf1-driven interactions peak within 10–20 min dissipate 1 hr presence 8.5% (v/v) ethanol, condensates are stably maintained hours. Moreover, same conditions, Pol II occupancy genes, chromatin remodeling, RNA expression detectable only later response much (>1 hr). This contrasts with coordinate thermal (39°C) where occupancy, transcription, histone eviction, interactions, Hsf1 all transient (peak 2.5–10 Therefore, forms condensates, restructures transcriptionally activates proteotoxic but does so strikingly different kinetics. subjected stress, repositions target before activating them.

Язык: Английский

Nuclear basket proteins Mlp1 and Nup2 drive heat shock-induced 3D genome restructuring DOI Creative Commons
Suman Mohajan, Linda S Rubio, David S. Gross

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

ABSTRACT The nuclear pore complex (NPC), a multisubunit located within the envelope, regulates RNA export and import of proteins. Here we address role NPC in driving thermal stress-induced 3D genome repositioning Heat Shock Responsive ( HSR ) genes yeast. We found that two basket proteins, Mlp1 Nup2, although dispensable for integrity, are required into coalesced chromatin clusters, consistent with their strong, heat shock-dependent recruitment to gene regulatory coding regions. clustering occurs predominantly nucleoplasm is independent essential scaffold-associated proteins Nup1 Nup145. Notably, double depletion Nup2 has little effect on formation Factor 1 (Hsf1)-containing transcriptional condensates, Hsf1 Pol II genes, or mRNA abundance. Our results define restructuring extrinsic downstream activation.

Язык: Английский

Процитировано

0
Nuclear Basket Proteins Mlp1 and Nup2 Drive Heat Shock–Induced 3D Genome Restructuring DOI Creative Commons
Suman Mohajan, Linda S Rubio, David S. Gross

и другие.

Опубликована: Янв. 31, 2025

The nuclear pore complex (NPC), a multisubunit located within the envelope, regulates RNA export and import of proteins. Here we address role NPC in driving thermal stress-induced 3D genome repositioning _Heat Shock Responsive_ (_HSR_) genes yeast. We found that two basket proteins, Mlp1 Nup2, although dispensable for integrity, are required _HSR_ into coalesced chromatin clusters, consistent with their strong, heat shock-dependent recruitment to gene regulatory coding regions. clustering occurs predominantly nucleoplasm is independent essential scaffold-associated proteins Nup1 Nup145. Notably, double depletion Nup2 has little effect on formation Heat Factor 1 (Hsf1)-containing transcriptional condensates, Hsf1 Pol II genes, or mRNA abundance. Our results define restructuring extrinsic downstream activation.

Язык: Английский

Процитировано

0
Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes DOI Creative Commons
Linda S Rubio, Suman Mohajan, David S. Gross

и другие.

eLife, Год журнала: 2024, Номер 12

Опубликована: Окт. 15, 2024

In insects and mammals, 3D genome topology has been linked to transcriptional states yet whether this link holds for other eukaryotes is unclear. Using both ligation proximity fluorescence microscopy assays, we show that in Saccharomyces cerevisiae , Heat Shock Response ( HSR ) genes dispersed across multiple chromosomes under the control of Factor (Hsf1) rapidly reposition cells exposed acute ethanol stress engage concerted, Hsf1-dependent intergenic interactions. Accompanying reconfiguration equally rapid formation Hsf1-containing condensates. However, contrast transience Hsf1-driven interactions peak within 10–20 min dissipate 1 hr presence 8.5% (v/v) ethanol, condensates are stably maintained hours. Moreover, same conditions, Pol II occupancy genes, chromatin remodeling, RNA expression detectable only later response much (>1 hr). This contrasts with coordinate thermal (39°C) where occupancy, transcription, histone eviction, interactions, Hsf1 all transient (peak 2.5–10 Therefore, forms condensates, restructures transcriptionally activates proteotoxic but does so strikingly different kinetics. subjected stress, repositions target before activating them.

Язык: Английский

Процитировано

3
Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes DOI Creative Commons
Linda S Rubio, Suman Mohajan, David S. Gross

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Ноя. 22, 2023

In insects and mammals, 3D genome topology has been linked to transcriptional states yet whether this link holds for other eukaryotes is unclear. Using both ligation proximity fluorescence microscopy assays, we show that in Saccharomyces cerevisiae , Heat Shock Response ( HSR ) genes dispersed across multiple chromosomes under the control of Factor (Hsf1) rapidly reposition cells exposed acute ethanol stress engage concerted, Hsf1-dependent intergenic interactions. Accompanying reconfiguration equally rapid formation Hsf1-containing condensates. However, contrast transience Hsf1-driven interactions peak within 10–20 min dissipate 1 hr presence 8.5% (v/v) ethanol, condensates are stably maintained hours. Moreover, same conditions, Pol II occupancy genes, chromatin remodeling, RNA expression detectable only later response much (>1 hr). This contrasts with coordinate thermal (39°C) where occupancy, transcription, histone eviction, interactions, Hsf1 all transient (peak 2.5–10 Therefore, forms condensates, restructures transcriptionally activates proteotoxic but does so strikingly different kinetics. subjected stress, repositions target before activating them.

Язык: Английский

Процитировано

3