Nutritional state-dependent modulation of insulin-producing cells in Drosophila
eLife,
Год журнала:
2025,
Номер
13
Опубликована: Янв. 29, 2025
Insulin
plays
a
key
role
in
metabolic
homeostasis.
Drosophila
insulin-producing
cells
(IPCs)
are
functional
analogues
of
mammalian
pancreatic
beta
and
release
insulin
directly
into
circulation.
To
investigate
the
vivo
dynamics
IPC
activity,
we
quantified
effects
nutritional
internal
state
changes
on
IPCs
using
electrophysiological
recordings.
We
found
that
strongly
modulates
activity.
activity
decreased
with
increasing
periods
starvation.
Refeeding
flies
glucose
or
fructose,
two
nutritive
sugars,
significantly
increased
whereas
non-nutritive
sugars
had
no
effect.
In
contrast
to
feeding,
perfusion
did
not
affect
This
was
reminiscent
incretin
effect,
where
ingestion
drives
higher
than
intravenous
application.
Contrary
IPCs,
Diuretic
hormone
44-expressing
neurons
pars
intercerebralis
(DH44
PI
Ns)
responded
perfusion.
Functional
connectivity
experiments
demonstrated
these
DH44
Ns
do
while
other
DH44Ns
inhibit
them.
Hence,
populations
autonomously
systemically
sugar-sensing
work
parallel
maintain
Accordingly,
activating
small,
satiety-like
effect
food-searching
behavior
reduced
starvation-induced
hyperactivity,
hyperactivity.
Taken
together,
demonstrate
an
integral
part
modulatory
network
orchestrates
homeostasis
adaptive
response
shifts
state.
Язык: Английский
Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila
Опубликована: Июль 2, 2024
Insulin
plays
a
key
role
in
metabolic
homeostasis.
Drosophila
insulin-producing
cells
(IPCs)
are
functional
analogues
of
mammalian
pancreatic
beta
and
release
insulin
directly
into
circulation.
To
investigate
the
vivo
dynamics
IPC
activity,
we
quantified
effects
nutritional
internal
state
changes
on
IPCs
using
electrophysiological
recordings.
We
found
that
strongly
modulates
activity.
activity
decreased
with
increasing
periods
starvation.
Refeeding
flies
glucose
or
fructose,
two
nutritive
sugars,
significantly
increased
whereas
non-nutritive
sugars
had
no
effect.
In
contrast
to
feeding,
perfusion
did
not
affect
This
was
reminiscent
incretin
effect,
where
ingestion
drives
higher
than
intravenous
application.
Contrary
IPCs,
Diuretic
hormone
44-expressing
neurons
pars
intercerebralis
(DH44PINs)
responded
perfusion.
Functional
connectivity
experiments
demonstrated
these
DH44PINs
do
while
other
DH44Ns
inhibit
them.
Hence,
populations
autonomously
systemically
sugar-sensing
work
parallel
maintain
Accordingly,
activating
small,
satiety-like
effect
food-searching
behavior
reduced
starvation-induced
hyperactivity,
hyperactivity.
Taken
together,
demonstrate
an
integral
part
modulatory
network
orchestrates
homeostasis
adaptive
response
shifts
state.
Язык: Английский
Nutritional state-dependent modulation of Insulin-Producing Cells inDrosophila
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 29, 2024
Insulin
plays
a
key
role
in
metabolic
homeostasis
across
vertebrate
and
invertebrate
species.
Drosophila
Insulin-Producing
Cells
(IPCs)
are
functional
analogues
to
mammalian
pancreatic
beta
cells
release
insulin
directly
into
circulation.
IPC
activity
is
modulated
by
nutrient
availability,
circadian
time,
the
behavioral
state.
To
investigate
vivo
dynamics
of
context
homeostasis,
we
quantified
effects
nutritional
internal
state
changes
on
IPCs
using
electrophysiological
recordings.
We
found
that
strongly
modulates
activity.
became
less
active
with
increasing
periods
starvation.
Refeeding
starved
flies
glucose
or
fructose,
two
nutritive
sugars,
significantly
increased
activity,
whereas
non-nutritive
sugar
protein
had
no
effect.
In
contrast
feeding,
perfusion
did
not
affect
This
was
reminiscent
incretin
effect,
which
ingestion
drives
higher
than
intravenous
application.
Contrary
IPCs,
Diuretic
hormone
44-expressing
neurons
pars
intercerebralis
(DH44
PI
Ns),
anatomically
similar
responded
perfusion.
Functional
connectivity
experiments
demonstrated
glucose-sensing
DH44
Ns
do
while
other
DH44Ns
inhibit
IPCs.
suggests
populations
autonomously
systemically
sugar-sensing
work
parallel
maintain
homeostasis.
Ultimately,
behavior.
For
example,
hungry
increase
their
locomotor
search
food.
support
this
idea,
activating
small,
satiety-like
effect
flies,
resulting
reduced
walking
Taken
together,
show
an
integral
part
sophisticated
modulatory
network
orchestrates
adaptive
behavior
response
shifts
Язык: Английский
A brief history of insect neuropeptide and peptide hormone research
Cell and Tissue Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Abstract
This
review
briefly
summarizes
50
years
of
research
on
insect
neuropeptide
and
peptide
hormone
(collectively
abbreviated
NPH)
signaling,
starting
with
the
sequencing
proctolin
in
1975.
The
first
25
years,
before
Drosophila
genome,
were
characterized
by
efforts
to
identify
novel
NPHs
biochemical
means,
mapping
their
distribution
neurons,
neurosecretory
cells,
endocrine
cells
intestine.
Functional
studies
predominantly
dealing
hormonal
aspects
peptides
many
employed
ex
vivo
assays.
With
annotation
more
specifically
receptors
other
insects,
a
new
era
followed.
started
matching
NPH
ligands
orphan
receptors,
localize
improved
detection
methods.
Important
advances
made
introduction
rich
repertoire
innovative
molecular
genetic
approaches
interfere
expression
or
function
receptors.
These
methods
enabled
cell-
circuit-specific
interference
signaling
for
assays
determine
roles
behavior
physiology,
imaging
neuronal
activity,
analysis
connectivity
peptidergic
circuits.
Recent
have
seen
dramatic
increase
reports
multiple
functions
development,
physiology
behavior.
Importantly,
we
can
now
appreciate
pleiotropic
NPHs,
as
well
functional
“networks”
where
state
dependent
ensures
behavioral
plasticity
systemic
homeostasis.
Язык: Английский
Substance P in nonmammalian biology: Evolutionarily conserved tachykinin signaling
Elsevier eBooks,
Год журнала:
2024,
Номер
unknown, С. 27 - 77
Опубликована: Ноя. 8, 2024
Язык: Английский
Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila
Опубликована: Дек. 19, 2024
Insulin
plays
a
key
role
in
metabolic
homeostasis
across
vertebrate
and
invertebrate
species.
Drosophila
Insulin-Producing
Cells
(IPCs)
are
functional
analogues
to
mammalian
pancreatic
beta
cells
release
insulin
directly
into
circulation.
IPC
activity
is
modulated
by
nutrient
availability,
circadian
time,
the
behavioral
state.
To
investigate
vivo
dynamics
of
context
homeostasis,
we
quantified
effects
nutritional
internal
state
changes
on
IPCs
using
electrophysiological
recordings.
We
found
that
strongly
modulates
activity.
became
less
active
with
increasing
periods
starvation.
Refeeding
starved
flies
glucose
or
fructose,
two
nutritive
sugars,
significantly
increased
activity,
whereas
non-nutritive
sugar
protein
had
no
effect.
In
contrast
feeding,
perfusion
did
not
affect
This
was
reminiscent
incretin
effect,
which
ingestion
drives
higher
than
intravenous
application.
Contrary
IPCs,
Diuretic
hormone
44-expressing
neurons
pars
intercerebralis
(DH44
PI
Ns),
anatomically
similar
responded
perfusion.
Functional
connectivity
experiments
demonstrated
glucose-sensing
DH44
Ns
do
while
other
DH44Ns
inhibit
IPCs.
suggests
populations
autonomously
systemically
sugar-sensing
work
parallel
maintain
homeostasis.
Ultimately,
behavior.
For
example,
hungry
increase
their
locomotor
search
food.
support
this
idea,
activating
small,
satiety-like
effect
flies,
resulting
reduced
walking
Taken
together,
show
an
integral
part
sophisticated
modulatory
network
orchestrates
adaptive
behavior
response
shifts
Язык: Английский