Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila DOI Open Access
Rituja S Bisen,

Fathima Mukthar Iqbal,

Federico Cascino-Milani

et al.

Published: Dec. 19, 2024

Insulin plays a key role in metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state. To investigate vivo dynamics of context homeostasis, we quantified effects nutritional internal state changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. became less active with increasing periods starvation. Refeeding starved flies glucose or fructose, two nutritive sugars, significantly increased activity, whereas non-nutritive sugar protein had no effect. In contrast feeding, perfusion did not affect This was reminiscent incretin effect, which ingestion drives higher than intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44 PI Ns), anatomically similar responded perfusion. Functional connectivity experiments demonstrated glucose-sensing DH44 Ns do while other DH44Ns inhibit IPCs. suggests populations autonomously systemically sugar-sensing work parallel maintain homeostasis. Ultimately, behavior. For example, hungry increase their locomotor search food. support this idea, activating small, satiety-like effect flies, resulting reduced walking Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts

Language: Английский

Nutritional state-dependent modulation of insulin-producing cells in Drosophila DOI Creative Commons
Rituja S Bisen,

Fathima Mukthar Iqbal,

Federico Cascino-Milani

et al.

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 29, 2025

Insulin plays a key role in metabolic homeostasis. Drosophila insulin-producing cells (IPCs) are functional analogues of mammalian pancreatic beta and release insulin directly into circulation. To investigate the vivo dynamics IPC activity, we quantified effects nutritional internal state changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. activity decreased with increasing periods starvation. Refeeding flies glucose or fructose, two nutritive sugars, significantly increased whereas non-nutritive sugars had no effect. In contrast to feeding, perfusion did not affect This was reminiscent incretin effect, where ingestion drives higher than intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44 PI Ns) responded perfusion. Functional connectivity experiments demonstrated these DH44 Ns do while other DH44Ns inhibit them. Hence, populations autonomously systemically sugar-sensing work parallel maintain Accordingly, activating small, satiety-like effect food-searching behavior reduced starvation-induced hyperactivity, hyperactivity. Taken together, demonstrate an integral part modulatory network orchestrates homeostasis adaptive response shifts state.

Language: Английский

Citations

1
Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila DOI Open Access
Rituja S Bisen,

Fathima Mukthar Iqbal,

Federico Cascino-Milani

et al.

Published: July 2, 2024

Insulin plays a key role in metabolic homeostasis. Drosophila insulin-producing cells (IPCs) are functional analogues of mammalian pancreatic beta and release insulin directly into circulation. To investigate the vivo dynamics IPC activity, we quantified effects nutritional internal state changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. activity decreased with increasing periods starvation. Refeeding flies glucose or fructose, two nutritive sugars, significantly increased whereas non-nutritive sugars had no effect. In contrast to feeding, perfusion did not affect This was reminiscent incretin effect, where ingestion drives higher than intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44PINs) responded perfusion. Functional connectivity experiments demonstrated these DH44PINs do while other DH44Ns inhibit them. Hence, populations autonomously systemically sugar-sensing work parallel maintain Accordingly, activating small, satiety-like effect food-searching behavior reduced starvation-induced hyperactivity, hyperactivity. Taken together, demonstrate an integral part modulatory network orchestrates homeostasis adaptive response shifts state.

Language: Английский

Citations

2
Nutritional state-dependent modulation of Insulin-Producing Cells inDrosophila DOI Creative Commons
Rituja S Bisen,

Fathima Mukthar Iqbal,

Federico Cascino-Milani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Insulin plays a key role in metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state. To investigate vivo dynamics of context homeostasis, we quantified effects nutritional internal state changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. became less active with increasing periods starvation. Refeeding starved flies glucose or fructose, two nutritive sugars, significantly increased activity, whereas non-nutritive sugar protein had no effect. In contrast feeding, perfusion did not affect This was reminiscent incretin effect, which ingestion drives higher than intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44 PI Ns), anatomically similar responded perfusion. Functional connectivity experiments demonstrated glucose-sensing DH44 Ns do while other DH44Ns inhibit IPCs. suggests populations autonomously systemically sugar-sensing work parallel maintain homeostasis. Ultimately, behavior. For example, hungry increase their locomotor search food. support this idea, activating small, satiety-like effect flies, resulting reduced walking Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts

Language: Английский

Citations

1
A brief history of insect neuropeptide and peptide hormone research DOI Creative Commons
Dick R. Nässel

Cell and Tissue Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Abstract This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing proctolin in 1975. The first 25 years, before Drosophila genome, were characterized by efforts to identify novel NPHs biochemical means, mapping their distribution neurons, neurosecretory cells, endocrine cells intestine. Functional studies predominantly dealing hormonal aspects peptides many employed ex vivo assays. With annotation more specifically receptors other insects, a new era followed. started matching NPH ligands orphan receptors, localize improved detection methods. Important advances made introduction rich repertoire innovative molecular genetic approaches interfere expression or function receptors. These methods enabled cell- circuit-specific interference signaling for assays determine roles behavior physiology, imaging neuronal activity, analysis connectivity peptidergic circuits. Recent have seen dramatic increase reports multiple functions development, physiology behavior. Importantly, we can now appreciate pleiotropic NPHs, as well functional “networks” where state dependent ensures behavioral plasticity systemic homeostasis.

Language: Английский

Citations

1
Substance P in nonmammalian biology: Evolutionarily conserved tachykinin signaling DOI
Dick R. Nässel

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 27 - 77

Published: Nov. 8, 2024

Language: Английский

Citations

0
Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila DOI Open Access
Rituja S Bisen,

Fathima Mukthar Iqbal,

Federico Cascino-Milani

et al.

Published: Dec. 19, 2024

Insulin plays a key role in metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state. To investigate vivo dynamics of context homeostasis, we quantified effects nutritional internal state changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. became less active with increasing periods starvation. Refeeding starved flies glucose or fructose, two nutritive sugars, significantly increased activity, whereas non-nutritive sugar protein had no effect. In contrast feeding, perfusion did not affect This was reminiscent incretin effect, which ingestion drives higher than intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44 PI Ns), anatomically similar responded perfusion. Functional connectivity experiments demonstrated glucose-sensing DH44 Ns do while other DH44Ns inhibit IPCs. suggests populations autonomously systemically sugar-sensing work parallel maintain homeostasis. Ultimately, behavior. For example, hungry increase their locomotor search food. support this idea, activating small, satiety-like effect flies, resulting reduced walking Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts

Language: Английский

Citations

0