Sulforaphane triggers Sirtuin 3-mediated ferroptosis in colorectal cancer cells via activating the adenosine 5‘-monophosphate (AMP)-activated protein kinase/ mechanistic target of rapamycin signaling pathway DOI Creative Commons
Bo Hu, Ping Cao,

Jinghui Wang

и другие.

Human & Experimental Toxicology, Год журнала: 2024, Номер 43

Опубликована: Янв. 1, 2024

Objective This study aimed to explore the expression and biological functions of SIRT3 in colorectal cancer cells (HCT-116), impacts sulforaphane on ferroptosis HCT-116 involvement SIRT3/AMPK/mTOR axis those effects. Methods SIRT3-overexpressing (OE) SIRT3-knockout (KO) cell lines were treated with different concentrations sulforaphane, RSL-3, IKE. Cell viability, intracellular ROS, MDA, iron levels, as well mRNA protein expressions target genes measured. Results was increased by inducers decreased inhibitors. overexpression reduced viability levels iron, whereas knockdown achieved opposite suppressed SLC7A11 promoted activation AMPK/mTOR pathway. Restoration blocked effects induction inhibition. antagonized inhibitors AMPK or mTOR. Moreover, triggered activating axis. Conclusions SLC7A11-mediated cells, reducing pathway, targets it inhibit cancer.

Язык: Английский

The dark side of SIRT7 DOI
Francisco Alejandro Lagunas‐Rangel

Molecular and Cellular Biochemistry, Год журнала: 2023, Номер 479(11), С. 2843 - 2861

Опубликована: Дек. 8, 2023

Язык: Английский

Процитировано

10
Sulforaphane triggers Sirtuin 3-mediated ferroptosis in colorectal cancer cells via activating the adenosine 5‘-monophosphate (AMP)-activated protein kinase/ mechanistic target of rapamycin signaling pathway DOI Creative Commons
Bo Hu, Ping Cao,

Jinghui Wang

и другие.

Human & Experimental Toxicology, Год журнала: 2024, Номер 43

Опубликована: Янв. 1, 2024

Objective This study aimed to explore the expression and biological functions of SIRT3 in colorectal cancer cells (HCT-116), impacts sulforaphane on ferroptosis HCT-116 involvement SIRT3/AMPK/mTOR axis those effects. Methods SIRT3-overexpressing (OE) SIRT3-knockout (KO) cell lines were treated with different concentrations sulforaphane, RSL-3, IKE. Cell viability, intracellular ROS, MDA, iron levels, as well mRNA protein expressions target genes measured. Results was increased by inducers decreased inhibitors. overexpression reduced viability levels iron, whereas knockdown achieved opposite suppressed SLC7A11 promoted activation AMPK/mTOR pathway. Restoration blocked effects induction inhibition. antagonized inhibitors AMPK or mTOR. Moreover, triggered activating axis. Conclusions SLC7A11-mediated cells, reducing pathway, targets it inhibit cancer.

Язык: Английский

Процитировано

1