Sulforaphane triggers Sirtuin 3-mediated ferroptosis in colorectal cancer cells via activating the adenosine 5‘-monophosphate (AMP)-activated protein kinase/ mechanistic target of rapamycin signaling pathway DOI Creative Commons
Bo Hu, Ping Cao,

Jinghui Wang

et al.

Human & Experimental Toxicology, Journal Year: 2024, Volume and Issue: 43

Published: Jan. 1, 2024

Objective This study aimed to explore the expression and biological functions of SIRT3 in colorectal cancer cells (HCT-116), impacts sulforaphane on ferroptosis HCT-116 involvement SIRT3/AMPK/mTOR axis those effects. Methods SIRT3-overexpressing (OE) SIRT3-knockout (KO) cell lines were treated with different concentrations sulforaphane, RSL-3, IKE. Cell viability, intracellular ROS, MDA, iron levels, as well mRNA protein expressions target genes measured. Results was increased by inducers decreased inhibitors. overexpression reduced viability levels iron, whereas knockdown achieved opposite suppressed SLC7A11 promoted activation AMPK/mTOR pathway. Restoration blocked effects induction inhibition. antagonized inhibitors AMPK or mTOR. Moreover, triggered activating axis. Conclusions SLC7A11-mediated cells, reducing pathway, targets it inhibit cancer.

Language: Английский

The dark side of SIRT7 DOI
Francisco Alejandro Lagunas‐Rangel

Molecular and Cellular Biochemistry, Journal Year: 2023, Volume and Issue: 479(11), P. 2843 - 2861

Published: Dec. 8, 2023

Language: Английский

Citations

10
Sulforaphane triggers Sirtuin 3-mediated ferroptosis in colorectal cancer cells via activating the adenosine 5‘-monophosphate (AMP)-activated protein kinase/ mechanistic target of rapamycin signaling pathway DOI Creative Commons
Bo Hu, Ping Cao,

Jinghui Wang

et al.

Human & Experimental Toxicology, Journal Year: 2024, Volume and Issue: 43

Published: Jan. 1, 2024

Objective This study aimed to explore the expression and biological functions of SIRT3 in colorectal cancer cells (HCT-116), impacts sulforaphane on ferroptosis HCT-116 involvement SIRT3/AMPK/mTOR axis those effects. Methods SIRT3-overexpressing (OE) SIRT3-knockout (KO) cell lines were treated with different concentrations sulforaphane, RSL-3, IKE. Cell viability, intracellular ROS, MDA, iron levels, as well mRNA protein expressions target genes measured. Results was increased by inducers decreased inhibitors. overexpression reduced viability levels iron, whereas knockdown achieved opposite suppressed SLC7A11 promoted activation AMPK/mTOR pathway. Restoration blocked effects induction inhibition. antagonized inhibitors AMPK or mTOR. Moreover, triggered activating axis. Conclusions SLC7A11-mediated cells, reducing pathway, targets it inhibit cancer.

Language: Английский

Citations

1