Host specificity, infrequent major host switching and the diversification of highly host‐specific symbionts: The case of vane‐dwelling feather mites

Global Ecology and Biogeography, Год журнала: 2017, Номер 27(2), С. 188 - 198

Опубликована: Ноя. 26, 2017

Abstract Aim Highly host‐specific symbionts are very rarely found except with their typical host species. Although switches to new hosts rare and difficult detect, a switch phylogenetically distant from the original one (a ‘major switch’) could allow diversification of onto lineage. The consequences such major on highly animals have been explored. Here, we examine specificity vane‐dwelling feather mites, group that shows strong specificity, together host‐switching dynamics for diversification. Location Global. Time period From 1882 2015. Major taxa studied Feather mites birds. Methods Using largest published dataset mite–bird associations, analysed raw, phylogenetic geographical mites. We by describing sharing bird species different distances. For three most species‐rich mite families, quantified Results Most (84%) inhabit closely related Assemblages birds do not show signature, but rather host‐driven structuring. probability occurs two decays sharply distance, only instance occupying distantly orders. However, results suggest despite few triggered origin 21% 38% genera families studied. Main conclusions symbionts, whose assemblages structure, even at continental scale. conclude events macroevolutionary diversity.

Язык: Английский

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Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean

PLoS ONE, Год журнала: 2018, Номер 13(3), С. e0195001 - e0195001

Опубликована: Март 29, 2018

Sponge-cyanobacteria associations have attracted research interest from an ecological, evolutionary and biotechnological perspective. Current knowledge is, in its majority, "hidden" metagenomics studying the entire microbial communities of sponges, while on these is totally missing for certain geographic areas. In this study, we (a) investigated occurrence cyanobacteria 18 sponge species, several which are studied first time their cyanobionts, a previously unexplored eastern Mediterranean ecoregion, Aegean Sea, (b) isolated sponge-associated cyanobacteria, characterized them based polyphasic (morphological-morphometric molecular phylogenetic analysis) approach, (c) conducted meta-analysis global diversity species hosting as well cyanobacterial symbionts. Our provided new records nine unknown association, were found to form novel clades within Synechococcus, Leptolyngbyaceae, Pseudanabaenaceae, Schizotrichaceae, whose taxonomic status requires further investigation; report Schizotrichaceae cyanobacterium associated with sponges. The extensive evaluation literature along data Sea raised number known 320 showed that reported sponges yet underestimated.

Язык: Английский

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Holobiont Diversity in a Reef-Building Coral over Its Entire Depth Range in the Mesophotic Zone

Frontiers in Marine Science, Год журнала: 2018, Номер 5

Опубликована: Фев. 7, 2018

Mesophotic reef-building coral communities (~30-120 m depth) remain largely unexplored, despite representing roughly three-quarters of the overall depth range at which tropical reef ecosystems occur. Although many species are restricted to shallow depths, several occur across large ranges, including lower mesophotic depths. Yet, it remains unclear how such can persist under extreme low-light conditions and different symbiotic partners associated with these corals contribute facilitate broad ranges. We assessed holobiont genetic diversity Caribbean Agaricia undata over in three localities Colombia: San Andres Island (between 37-85m), Cartagena 17-45m) "Parque Nacional Natural Corales de Profundidad"(between 77-87m). used a population genomics approach (NextRAD) for host, amplicon sequencing Symbiodinium (non-coding region plastid psbA minicircle) prokaryotic (V4 16S rRNA gene) symbiont community. For structuring was only observed geographic regions, but not between Bayesian clustering discriminant analysis principal components revealed (60 m) This pattern confirmed when evaluating pairwise differentiation (FST) populations, much higher values regions (0.0467-0.1034) compared depths (within location; -0.0075- [-0.0007]). Symbiotic partners, seven types zooxanthellae 325 OTUs, did exhibit partitioning All samples hosted clade C3 type C3psbA_e present all Alpha microbial significantly zones (upper vs lower), community composition colonies similar two (ANOSIM, R = -0.079, P > 0.05). The microbiome dominated by Uncultured Betaproteobacteria order EC94 (16%), Unknown-Bacteria (15%), family Cenarchaeaceae (12 %), Burkholderiaceae (10%) Hahellaceae (10%). constant coral-holobiont along studied suggests that identity symbionts is responsible coral's adaptation low light environments.

Язык: Английский

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Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Март 1, 2022

Abstract Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many these compounds. Yet, environmental influences on sponge-associated compound production remain elusive. Here, we investigated changes metabolomes in along a depth range 1232 m. Using 16S rRNA gene amplicon sequencing untargeted metabolomics, assessed prokaryotic chemical diversities three deep-sea sponge species: Geodia barretti , Stryphnus fortis Weberella bursa . Both communities metabolome varied significantly with depth, which hypothesized effect different water masses. Up 35.5% microbial ASVs (amplicon sequence variants) showed significant while phylum-level composition host microbiome remained unchanged. The relative quantities known increasing or decreasing strongly. Other metabolites varying were compatible solutes regulating osmolarity cells. Correlations between community G. suggested members Acidobacteria, Proteobacteria, Chloroflexi, an unclassified prokaryote as potential producers.

Язык: Английский

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Archaeal and bacterial diversity and community composition from 18 phylogenetically divergent sponge species in Vietnam

PeerJ, Год журнала: 2018, Номер 6, С. e4970 - e4970

Опубликована: Июнь 8, 2018

Sponge-associated prokaryotic diversity has been studied from a wide range of marine environments across the globe. However, for certain regions, e.g., Vietnam, Thailand, Cambodia, and Singapore, an overview sponge-associated communities is still pending. In this study we characterized 27 specimens, comprising 18 sponge species, sampled central coastal region Vietnam. Illumina MiSeq sequencing 16S ribosomal RNA (rRNA) gene fragments was used to investigate bacterial archaeal diversity. Overall, 14 phyla one phylum were identified among all samples. The Proteobacteria present in sponges most prevalent 15 out albeit with pronounced differences at class level. contrast, Chloroflexi abundant Halichondria sp., whereas Spirastrella sp. Dactylospongia dominated by Actinobacteria . Several such as Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Deferribacteres, Gemmatimonadetes , Nitrospirae found two-thirds species. Moreover, Thaumarchaeota (Archaea), which known comprise nitrifying archaea, highly majority investigated Altogether, demonstrates that associated Vietnamese comparable sponge-prokaryotic assemblages well-documented regions. Furthermore, phylogenetically divergent hosted species-specific communities, thus demonstrating influence host identity on composition communities. Therefore, high-throughput rRNA amplicon analysis provides foundation future studies symbiont function sponge-derived bioactive compounds region.

Язык: Английский

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Prokaryotic communities of Indo-Pacific giant barrel sponges are more strongly influenced by geography than host phylogeny

FEMS Microbiology Ecology, Год журнала: 2018, Номер 94(12)

Опубликована: Окт. 3, 2018

Sponges harbor complex communities of microorganisms that carry out essential roles for the functioning and survival their hosts. In some cases, genetically related sponges from different geographic regions share microbes, while in other cases microbial are more similar unrelated collected same location. To better understand how geography host phylogeny cause variation prokaryotic community sponges, we compared 44 giant barrel (Xestospongia spp.). These belonged to six reproductively isolated genetic groups eight areas throughout Indo-Pacific region. Using Illumina sequencing, obtained 440 000 sequences 16S rRNA gene V3V4 variable region were assigned 3795 operational taxonomic units (OTUs). The was characterized by 71 core OTUs (i.e. present each specimen) represented 57.5% total number sequences. relative abundance these varied significantly among samples, this predominantly origin sample. results show is primarily associated with as opposed phylogenetic relatedness.

Язык: Английский

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Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges—A Review

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Ноя. 10, 2021

The application of high-throughput microbial community profiling as well “omics” approaches unveiled high diversity and host-specificity bacteria associated with marine sponges, which are renowned for their wide range bioactive natural products. However, exploration exploitation compounds from sponge-associated have been limited because the majority remains recalcitrant to cultivation. In this review, we (i) discuss recent/novel cultivation techniques that used isolate bacteria, (ii) provide an overview isolated sponges until 2017 culture conditions identify not yet cultured (iii) outline promising strategies cultivating uncultivated in future. Despite intensive attempts, obtained through much lower than seen cultivation-independent methods, is particularly noticeable those taxa were previously marked “sponge-specific” “sponge-enriched.” This poses urgent need more efficient methods. Refining media based on information metagenomic datasets under simulated most wanted bacteria.

Язык: Английский

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A novel Chromatiales bacterium is a potential sulfide oxidizer in multiple orders of marine sponges

Environmental Microbiology, Год журнала: 2017, Номер 20(2), С. 800 - 814

Опубликована: Дек. 1, 2017

Sponges are benthic filter feeders that play pivotal roles in coupling benthic-pelagic processes the oceans involve transformation of dissolved and particulate organic carbon nitrogen into biomass. While contribution sponge holobionts to cycle has been recognized past years, their importance sulfur cycle, both oceanic physiological, only recently gained attention. general, Theonella swinhoei particular, harbour a multitude associated microorganisms could affect cycling within holobiont. We reconstructed genome Chromatiales (class Gammaproteobacteria) bacterium from metagenomic sequence dataset T. swinhoei-associated microbial community. This relatively abundant metabolic capability oxidize sulfide yet displays reduced potential suggestive its lifestyle as an obligatory symbiont. was detected multiple orders, according similarities key genes such 16S rRNA polyketide synthase genes. Due oxidation metabolism occurrence many members Porifera phylum, we suggest naming newly described taxon Candidatus Porisulfidus.

Язык: Английский

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Identification, classification, and functional characterization of novel sponge-associated acidimicrobiial species

Systematic and Applied Microbiology, Год журнала: 2023, Номер 46(4), С. 126426 - 126426

Опубликована: Апрель 28, 2023

Язык: Английский

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Sponge-associated microbes in the twilight zone of Curaçao

Symbiosis, Год журнала: 2024, Номер 93(1), С. 81 - 97

Опубликована: Май 1, 2024

Abstract Mesophotic reefs, located in the "Twilight Zone" of ocean, are coral reefs that exist relatively deep waters ranging from approximately 30 to 150 m below surface. These situated just beyond reach conventional SCUBA diving and typically explored using advanced techniques or with aid submersibles. In present study, we used a state-of-the-art submersible sample 26 sponge specimens belonging 11 species. High (HMA) low (LMA) microbial abundance status was assigned species based on TEM imagery. Prokaryotic communities associated these sponges were, furthermore, assessed high-throughput sequencing. Proteobacteria, Chloroflexi, Actinobacteriota, Acidobacteriota were most abundant phyla overall. HMA/LMA proved be highly significant predictor prokaryotic composition. HMA also tended more diverse terms richness evenness than LMA sponges. 14 predictor-classes identified an exploratory technique machine learning including classes within Chloroflexi (e.g., Dehalococcoidia JG30-KF-CM66) (Thermoanaerobaculia Subgroups 21). Previous studies have demonstrated prevalence dichotomy shallow and, recently, sea. Our results demonstrate its mesophotic realm.

Язык: Английский

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